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Method for measuring naringin content in preparation for reunion of bone

A technique of bone-bonding and tendon extension and measurement methods, which can be used in anti-inflammatory agents, bone diseases, measuring devices, etc., can solve the problems of lack of content determination methods, and achieve the effects of strong specificity, short measurement time, and improved quality

Active Publication Date: 2009-07-22
山西华元医药生物技术有限公司
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0002] Jiegu Xujin Tablets are recorded on page 170 of volume 11 of the Ministry of Health's drug standard "Prescriptions of Traditional Chinese Medicine", standard number WS 3 -B-2226-96, the prescription is Lizard 500g, Drynaria 333g, Panshanlong 167g, the dosage form is sugar-coated tablet, function of promoting blood circulation and removing blood stasis, reducing swelling and relieving pain, mainly treating soft tissue injury, fracture, etc., but lacks content determination method

Method used

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  • Method for measuring naringin content in preparation for reunion of bone
  • Method for measuring naringin content in preparation for reunion of bone
  • Method for measuring naringin content in preparation for reunion of bone

Examples

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Effect test

Embodiment 1

[0041] Jiegu Xujin Tablets (according to the Ministry of Health's drug standard WS 3 - Determination of naringin content in sugar-coated tablets prepared by the prescription of B-2226-96).

[0042] 1) Chromatographic conditions and system suitability test:

[0043] Chromatographic column: octadecylsilane bonded silica gel column (5μm, 4.6mm×200mm);

[0044] Mobile phase: methanol: 2.25% acetic acid solution = 35:65;

[0045] Flow rate: 0.9mL·min -1 ;

[0046] Detection wavelength: 283nm;

[0047] Column temperature: 35°C.

[0048] The number of theoretical plates is calculated according to the naringin peak and should not be less than 2000.

[0049] 2) Preparation of reference substance solution: Precisely weigh 10 mg of naringin reference substance dried to constant weight at 110°C, put it in a 100 mL volumetric flask, add methanol to the mark, shake well, accurately measure 3 mL, and place it in a 10 mL volumetric flask , add methanol 2mL, and then dilute to the mark ...

Embodiment 2

[0055] Jiegu Xujin Capsules (according to the Ministry of Health's drug standard WS 3 - Determination of the content of naringin in capsules made from the prescription of B-2226-96, specification: 0.4g per capsule).

[0056] 1) Chromatographic conditions and system suitability test:

[0057] Chromatographic column: octadecylsilane bonded silica gel column (10μm, 4.6mm×250mm);

[0058] Mobile phase: methanol: 2.25% acetic acid solution = 38: 62;

[0059] Flow rate: 1.0mL·min -1 ;

[0060] Detection wavelength: 283nm;

[0061] Column temperature: 40°C.

[0062]The number of theoretical plates is calculated according to the naringin peak and should not be less than 2000.

[0063] 2) Preparation of reference substance solution: accurately weigh 3 mg of naringin reference substance, put it in a 10 mL measuring bottle, add methanol to the mark, shake well, accurately measure 1 mL, put it in a 10 mL measuring bottle, add 4 mL of methanol, and then use 2.25 mL of methanol. The ...

Embodiment 3

[0069] Jiegu Xujin Capsules (according to the Ministry of Health's drug standard WS 3 - Determination of the content of naringin in capsules made from the prescription of B-2226-96, specification: 0.4g per capsule).

[0070] 1) Chromatographic conditions and system suitability test:

[0071] Chromatographic column: octadecylsilane bonded silica gel column (10μm, 4.6mm×250mm);

[0072] Mobile phase: methanol: 2.25% acetic acid solution = 40: 60;

[0073] Flow rate: 1.2mL·min -1 ;

[0074] Detection wavelength: 283nm;

[0075] Column temperature: 45°C.

[0076] The number of theoretical plates is calculated according to the naringin peak and should not be less than 2000.

[0077] 2) Preparation of reference substance solution: Precisely weigh 15 mg of naringin reference substance dried at 110°C to constant weight, put it in a 50 mL measuring bottle, add methanol to the mark, shake well, accurately measure 1 mL, and place it in a 10 mL measuring bottle , add methanol 4mL, ...

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Abstract

The invention relates to a method for measuring the content of naringoside in a muscle and bone reunion preparation. The method selects the naringoside as an index composition of rhizoma drynariae in the muscle and bone reunion preparation, uses methanol and an acetic acid solution to prepare a reference substance solution of the naringoside and a test sample solution of the muscle and bone reunion preparation, adopts octadecyl silane bonded silica gel column to carry out high-efficiency liquid phase chromatography mensuration under the 283 nm detection wavelength by taking the ratio of the methanol to 1.5 to 2.5 percent the acetic acid solution as 35:65 to 40:60 as a mobile phase, and uses an external standard method to calculate the content of the naringoside in a muscle and bone reunion preparation sample. The established mensuration method has strong specificity, good linear relation, high accuracy, good repeatability and short measuring time, can scientifically evaluate the transfer rate of the naringoside according to the method, and effectively improve the quality of a product.

Description

technical field [0001] The invention belongs to the technical field of content determination of Chinese patent medicines, and relates to a method for content determination of Chinese patent medicines containing Rhizoma Drynariae, in particular to determination of the content of Rhizoma Drynariae in preparations for connecting bones and extending tendons. The invention specifically takes the content determination method of naringin in Rhizoma Drynariae as an index component. Background technique [0002] Jiegu Xujin Tablets are included in the Ministry of Health's drug standard "Chinese Medicine Formulations", Volume 11, Page 170, Standard No. WS 3 -B-2226-96, the prescription is Lizard 500g, Drynaria 333g, Pangshanlong 167g, the dosage form is sugar-coated tablet, which functions to promote blood circulation and remove blood stasis, reduce swelling and relieve pain, and treat soft tissue injury, fracture, etc., but lack of content determination method. [0003] Rhizoma Dryn...

Claims

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Application Information

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IPC IPC(8): A61K36/8945A61P19/08A61P29/00G01N30/36A61K35/56A61K35/583
Inventor 朱平李娜王志敏
Owner 山西华元医药生物技术有限公司