Microfluidic chip-based microbial detection instrument and its spr detection method

Abstract

The invention discloses a microfluidic chip-based microorganism detection instrument and an SPR detection method thereof, wherein the microfluidic chip-based microorganism detection instrument comprises an incident light source, an optical fiber collimator, a multi-channel collimator, a multi-channel spectrometer, a lens And a microfluidic chip with surface plasmon resonance response characteristics. The microfluidic chip has a fluid microchannel, and a metal film array layer is arranged in the microfluidic chip. A variety of microbial antibody layers are fixed on the metal film array layer, and the lens is close to the surface. Located on one side of the metal film array layer on the microfluidic chip, the light emitted by the incident light source passes through the fiber optic collimator and lens in turn and then irradiates the metal film array layer, and then is reflected by the lens to the multi-channel collimator and then enters the microfluidic chip. Multi-channel spectrometer. The invention highly integrates the microfluidic chip and the lens, can detect the microbial species and the concentrations of different components contained in a large number of samples at one time, the samples do not need to be marked, the detection speed is fast, and the detection accuracy is high.

Description

technical field [0001] The invention relates to a microorganism detection instrument based on a microfluidic chip and an SPR detection method thereof, belonging to the fields of industrial testing and environmental monitoring. Background technique [0002] At present, if our daily drinking water and food are contaminated by microorganisms, these contaminated water and food enter the intestinal tract through the mouth, multiply and emit toxins in the intestinal tract, destroy the intestinal mucosal tissue, and cause intestinal dysfunction and damage. Seriously affect health. Once the human body is infected, the microbial eggs will be excreted from the patient's feces and will infect others again, leading to the outbreak of larger-scale infectious diseases. Traditional microbial detection methods mainly include plate counting method, immunoassay method and PCR method, etc. The plate counting method mixes the diluted microorganisms with the medium to grow and multiply into mul...

AI Technical Summary

Problems solved by technology

The common disadvantages of these methods are that it takes a long time, involves tedious biochemical reaction process, requires a variety of chemical reagents and professionals to participate, and cannot meet the needs of rapid and accurate microbial detection.

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