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Detection method of corydalis impatiens medicinal material

A detection method, the technology of Corydalis medicinal materials, applied in the field of detection of medicinal materials, can solve the problems of no content determination index, backward quality standards of Viola Corydalis, and the inability to accurately and effectively control the quality of medicinal materials, so as to avoid interference, increase accuracy and scientifically sexual effect

Active Publication Date: 2014-03-05
SHANDONG JINHE DRUG RES DEV
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0005] The technical problem to be solved by the present invention is that the current quality standard of Viola Corydalis is relatively backward, and there are only properties and thin-layer identification items, without any content measurement index, so the quality of the medicinal material cannot be accurately and effectively controlled, and a Tibetan medicinal material Saibei The quality detection method of Corydalis

Method used

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  • Detection method of corydalis impatiens medicinal material

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Experimental program
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Effect test

Embodiment 1

[0119] The Tibetan medicinal material Corydalis Saibei was provided by Qinghai Jinhe Tibetan Medicine Pharmaceutical Co., Ltd.

[0120] Determination of total alkaloid content

[0121] (1) Configuration of reference substance solution Precisely weigh 5 mg of pro-opiate that has been dried to constant weight, add 1 ml of sulfuric acid solution (1→100) to dissolve, dilute to 100 ml with water, shake well, and obtain.

[0122] (2) Preparation of standard solution Precisely draw 0, 1, 2, 3, 4, 5ml of the reference solution, put them in the separating funnel respectively, add 0.1% (mass volume ratio, that is, the content of bromocresol green in 100ml solution) 0.1g) bromocresol green test solution 2ml, phosphate buffer 2ml, make up the solution to 10ml with water. Add 10ml of dichloromethane, shake for 2min, let it stand still, separate the organic layer and measure the absorbance at a wavelength of 410nm, and draw a standard curve.

[0123] (3) Preparation of sample solution Tak...

Embodiment 2

[0134] The Tibetan medicinal material Corydalis Saibei was provided by Qinghai Jinhe Tibetan Medicine Pharmaceutical Co., Ltd.

[0135] Determination of total alkaloid content

[0136] (1) Configuration of reference substance solution Precisely weigh 8mg of pro-opiate which has been dried to constant weight, add 1ml of sulfuric acid solution (1→100) to dissolve, dilute to 100ml with water, shake well, and get ready.

[0137] (2) Preparation of standard solution Precisely draw 0, 1, 2, 3, 4, 5ml of the reference solution, put them in the separating funnel respectively, add 0.1% (mass volume ratio, that is, the content of bromocresol green in 100ml solution) 0.1g) bromocresol green test solution 2ml, phosphate buffer 2ml, make up the solution to 10ml with water. Add 10ml of dichloromethane, shake for 2min, let it stand still, separate the organic layer and measure the absorbance at a wavelength of 410nm, and draw a standard curve.

[0138] (3) Preparation of sample solution Ta...

Embodiment 3

[0148] The Tibetan medicinal material Corydalis Saibei was provided by Qinghai Jinhe Tibetan Medicine Pharmaceutical Co., Ltd.

[0149] Determination of total alkaloid content

[0150] (1) Configuration of reference substance solution Precisely weigh 6 mg of pro-opiate which has been dried to constant weight, add 1 ml of sulfuric acid solution (1→100) to dissolve, dilute to 100 ml with water, shake well, and obtain.

[0151] (2) Preparation of standard solution Precisely draw 0, 1, 2, 3, 4, 5ml of the reference solution, put them in the separating funnel respectively, add 0.1% (mass volume ratio, that is, the content of bromocresol green in 100ml solution) 0.1g) bromocresol green test solution 2ml, phosphate buffer 2ml, make up the solution to 10ml with water. Add 10ml of dichloromethane, shake for 2min, let it stand still, separate the organic layer and measure the absorbance at a wavelength of 410nm, and draw a standard curve.

[0152] (3) Preparation of sample solution Ta...

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Abstract

The invention discloses a detection method of a corydalis impatiens medicinal material. The detection method comprises the following steps: measuring the content of total alkaloids of the corydalis impatiens medicinal material through an ultraviolet spectrophotometric method, preparing a protopine reference solution, preparing a standard solution, making a standard curve and preparing a sample solution, wherein the preparation method of the sample solution comprises the steps of: backflow extracting corydalis impatiens medicinal material fine powder by using ethanol, dissolving filtered residue by using a sulfuric acid solution to obtain the sample solution, measuring the absorbance of the sample solution at 410nm wavelength, and calculating the content of the total alkaloids in the medicinal material according to the standard curve. According to the detection method disclosed by the invention, the protopine is used as reference; in a preparation process of the test sample, interference factors are removed by means of heating backflow and sulfuric acid solution dissolution, a characteristic reaction is carried out on alkaloid, bromocresol green and a phosphate buffer solution, the product of the characteristic reaction is extracted by dichloromethane to be measured; and since the dichloromethane only extracts the product generated in the characteristic reaction, the interference of non-alkaloid components is avoided and the accuracy and scientificity of the result are increased.

Description

technical field [0001] The invention relates to a method for detecting medicinal materials, in particular to a method for detecting Tibetan medicinal materials Corydalis cypress. Background technique [0002] There are about 320 species of Corydalis in the world, mainly produced in Asia. There are about 200 species in my country, and they are distributed all over the country. Corydalis saibei is the dried whole plant of Corydalis saibeiensis (also known as Corydalis saibeiensis), which is distributed in Qinghai, Tibet, Gansu, Sichuan and other places. It is a commonly used drug in Tibetan medicine, and the data shows that 25% It is used in the treatment of various hemorrhage, hepatitis, cholecystitis, influenza and other diseases. It is also used to treat bruises or other trauma, fever, gastroenteritis and so on. [0003] Judging from the current research results on the chemical components of this genus, this genus mainly contains prototropines, protoberberines, phenolisoqu...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N21/33G01N30/02G01N1/28G01N30/06
Inventor 李怀平任松鹏单玉刚魏永义
Owner SHANDONG JINHE DRUG RES DEV
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