Method for extracting dinophysistoxins-1 and yessotoxin-2 from seawater and purifying dinophysistoxins-1 and yessotoxin-2

A technology of fin algae toxin and scallop toxin, applied in chemical instruments and methods, separation methods, organic chemistry, etc., can solve the problems of difficult cultivation of algae, difficulty in preparation process, and limited number of one-time cultivation, etc., and achieve the effect of simple method

Active Publication Date: 2014-03-12
YELLOW SEA FISHERIES RES INST CHINESE ACAD OF FISHERIES SCI
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Problems solved by technology

The preparation process is difficult because the algae that produce fintoxin-1 and scalloptoxin-2 are difficult to cultivate under laboratory conditions, and the number of cultures at one time is also limited.

Method used

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  • Method for extracting dinophysistoxins-1 and yessotoxin-2 from seawater and purifying dinophysistoxins-1 and yessotoxin-2
  • Method for extracting dinophysistoxins-1 and yessotoxin-2 from seawater and purifying dinophysistoxins-1 and yessotoxin-2
  • Method for extracting dinophysistoxins-1 and yessotoxin-2 from seawater and purifying dinophysistoxins-1 and yessotoxin-2

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Embodiment Construction

[0021] A method for extracting and preparing fintoxin-1 and scallop toxin-2 from seawater, the specific steps are as follows:

[0022] 1) Adsorption: Use polyester mesh with a pore size of 48 μm to sew an adsorption bag into a square bag with a side length of 10cm×10cm, and weigh 100g of the macropore with non-polar polystyrene-divinylbenzene as the matrix Adsorbent resin (HP20, Mitsubishi Chemical Co., Ltd.) was placed in the adsorption bag, then sealed, and a nylon hook was fixed at one corner of the adsorption bag. Hang 20 adsorption bags on the floats in the Lingshan Bay area of ​​the Yellow Sea in June, 0.5-1m below the surface of the seawater, soak them for 7 days and take them out;

[0023] 2) Transfer 10 g of the macroporous resin in the adsorption bag to a glass chromatography column with a 4F piston with a sand core (inner diameter 22 mm, length 200 mm-300 mm, Shanghai Chubo Laboratory Equipment Co., Ltd.) without a standard port, and use 50 Rinse the sand core filt...

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Abstract

The invention relates to a method for extracting dinophysistoxins-1 and yessotoxin-2 from seawater and purifying the dinophysistoxins-1 and the yessotoxin-2. The method specifically comprises the follow steps: (1), an adsorption bag is made by using a piece of polyester wire mesh cloth with the bore diameter of 48 mum through sewing, the interior of the adsorption bag is filled with adsorption resin, and then sealing and absorbing are performed; (2), a glass sand core chromatographic column is used, and firstly deionized water is used for removing salt in the adsorption resin; (3), a glass sand core chromatographic column filled with an aluminium oxide filler with the granularity of 75 to 150 mun is used for purification treatment; (4), a glass sand core chromatographic column filled with reverse phase silica gel C18 is used for purification treatment so as to obtain a mixture containing the dinophysistoxins-1 and the yessotoxin-2. The method provided by the invention has the benefits that two matters of the dinophysistoxins-1 and the yessotoxin-2 can be prepared simultaneously, can be used as mixed standard substances, are free from the limitation of quantities of collected alga and shellfishesm, and can be extracted and prepared in batch from seawater in natural sea areas, and the method is simple and effective.

Description

technical field [0001] The invention relates to a method for extracting fin algae toxin-1 and scallop toxin-2 from seawater. Background technique [0002] Diarrhetic Shellfish Poisoning (DSP) is a fat-soluble polycyclic ether biologically active substance produced by some species of red tide algae Pynoflagellate and Prorocentrum. DSP causes diarrhea and vomiting in those who eat it, and the symptoms usually disappear within a few days, and the patient can recover on his own. DSP often appears in the coastal areas of our country, and it is also one of the main types of toxins monitored by countries all over the world. Whether it is reliable monitoring and analysis of toxins, or the development of new toxin monitoring and analysis techniques, especially the preparation of toxin rapid detection kits, a large number of toxin standards must be used. [0003] At present, there is no preparation technology for Dinophysistoxin-1 (DTX-1) and Pectenotoxin-2 (Pectenotoxin-2, PTX-2) i...

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): B01D15/00B01D15/08C07D519/00C07D493/22
Inventor 李兆新郭萌萌吴海燕王英姿
Owner YELLOW SEA FISHERIES RES INST CHINESE ACAD OF FISHERIES SCI
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