Fermentation medium for improving yield of gamma-linolenic acid produced from mucorcircinelloides and use

A fermentation medium and a technology of Mucor curcumae, applied in the field of microorganisms, can solve the problem that γ-linolenic acid is rare and the like

Inactive Publication Date: 2014-03-19
JIANGNAN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] At present, the research on increasing the production of γ-linolenic acid by adding amino acid as nitrogen source is rare at home and abroad

Method used

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  • Fermentation medium for improving yield of gamma-linolenic acid produced from mucorcircinelloides and use
  • Fermentation medium for improving yield of gamma-linolenic acid produced from mucorcircinelloides and use
  • Fermentation medium for improving yield of gamma-linolenic acid produced from mucorcircinelloides and use

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0102] A. Activation of bacteria

[0103] Under sterile conditions, take out the glycerol tube of Mucor circinifolia CBS108.16 spores from the -80°C ultra-low temperature refrigerator, draw 100 μL into a baffled Erlenmeyer flask containing 150 mL of the following activation medium, and use it in a laboratory provided by Shanghai Zhicheng In the shaker sold by Analytical Instrument Manufacturing Co., Ltd. under the trade name of ZHWY-2102 rotary constant temperature shaker, it was cultured at a constant temperature at a temperature of 30°C and a rotation speed of 150rpm for 24 hours to obtain an activated bacterial seed solution.

[0104] B. Seed cultivation

[0105] Under sterile conditions, according to the volume of the activated bacterial seed solution obtained in step A is 10% of the volume of the seed culture medium, the activated bacterial seed liquid is inserted into the tape baffle Erlenmeyer flask with 150mL seed medium , in the shaker at a temperature of 30° C. and ...

Embodiment 2

[0114] A. Activation of bacteria

[0115] Under sterile conditions, take out the glycerol tube of Mucor circinifolia CBS108.16 spores from the ultra-low temperature refrigerator at -80°C, pipette 100 μL into a baffled Erlenmeyer flask containing 100 mL of the following activation medium, and prepare it in Shanghai Zhicheng In the shaker sold by Analytical Instrument Manufacturing Co., Ltd. under the trade name of ZHWY-2102 rotary constant temperature shaker, it was cultured at a constant temperature at a temperature of 30 ° C and a rotation speed of 200 rpm for 16 hours to obtain an activated bacterial seed solution.

[0116] B. Seed cultivation

[0117] Under sterile conditions, according to the volume of the activated bacterial seed solution obtained in step A is 10% of the volume of the seed culture medium, the activated bacterial seed liquid is inserted into the tape baffle Erlenmeyer flask with 150mL seed medium , and cultivated at a constant temperature for 16 hours und...

Embodiment 3

[0126] A. Activation of bacteria

[0127] Under sterile conditions, take out the glycerol tube of Mucor circinifolia CBS108.16 spores from the ultra-low temperature refrigerator at -80°C, draw 100 μL into a baffled Erlenmeyer flask containing 200 mL of the following activation medium, and use it in a laboratory provided by Shanghai Zhicheng In the shaker sold by Analytical Instrument Manufacturing Co., Ltd. under the trade name of ZHWY-2102 rotary constant temperature shaker, it was cultured at a constant temperature at a temperature of 30°C and a rotation speed of 150rpm for 16 hours to obtain an activated bacterial seed solution.

[0128] B. Seed cultivation

[0129] Under sterile conditions, according to the volume of the activated bacterial seed liquid obtained in step A is 5% of the volume of the seed culture medium, the activated bacterial seed liquid is inserted into the tape baffle Erlenmeyer flask with 100mL seed medium , in the shaker at a temperature of 30° C. and ...

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Abstract

The invention relates to a fermentation medium for improving the yield of gamma-linolenic acid produced from mucorcircinelloides CBS108.16. The fermentation medium contains amino acid nitrogen sources of tyrosine, asparagine or aspartic acid. The invention also relates to a method for improving the yield of gamma-linolenic acid produced from the mucorcircinelloides CBS108.16 by using the fermentation medium. The method comprises the steps of strain activation, seed culture, fermentation culture, drying and the like. According to the fermentation medium and the method, the biomass of a mucorcircinelloides CBS108.16 thallus reaches 16.5 to 19.8g/L, and is increased by 25 to 52 percent compared with that of a control group; the fatty acid content of the mucorcircinelloides CBS108.16 thallus reaches 24 to 35 percent, and is increased by 11 to 48 percent compared with that of the control group; the content of gamma-linolenic acid in fermentation broth reaches 810 to 1300mg/L, and is increased by 41 to 124 percent compared with that of the control group. An operating process is simple and convenient, requirements on processing equipment are low, and the yield is remarkably improved, so that the method has broad application prospect.

Description

【Technical field】 [0001] The invention belongs to the technical field of microorganisms. More specifically, the invention relates to a fermentation medium for increasing the yield of γ-linolenic acid produced by Mucor circinosa CBS108.16, and also relates to using the fermentation medium to increase CBS108.16 Method for producing γ-linolenic acid. 【Background technique】 [0002] As an important unsaturated fatty acid, γ-linolenic acid (cis-6,9,12-octadecatrienoic acid) is a structural material of biofilms in various tissues of the human body and a precursor for the synthesis of prostaglandins. γ-linolenic acid plays an important role in nutrition and health care. It can resist cardiovascular disease, lower blood fat, lower blood sugar, fight cancer, whiten skin and resist skin aging. Its potential medicinal value has attracted widespread attention. [0003] Natural γ-linolenic acid mainly exists in borage, evening primrose and black currant, and the content of γ-linolenic a...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12P7/64C12R1/785
Inventor 宋元达唐鑫陈卫陈海琴张怀渊
Owner JIANGNAN UNIV
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