Cross breeding method for river odontobutis obscurus and marbled sand gobies
A technology of Channa japonica and Channa praecox, which is applied in the field of distant hybridization of fish, crossbreeding of fishes from different families of Channa chinensis and Channa praecox in rivers, and can solve the problem of low yield per mu and inability to adapt Breeding and other issues to achieve the effect of fast growth
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[0016] 1. Select parents:
[0017] Select a robust individual, no disease, no disability, no parasites on the body surface, good nutritional status, the size is 45g-120g, in the mature stage of gonad development, the river sand pond snakehead above 1 year old is used as the female parent; the healthy individual is selected , no disease, no disability, no parasites on the body surface, good nutritional status, and the specification is more than 350g. In the mature stage of gonad development, Channa japonica is used as the male parent.
[0018] 2. Artificial induction of labor, egg squeezing, and sperm collection:
[0019] 15 days before induction of labor, the broodstock were injected with exogenous hormone LRH-A (luteinizing hormone releasing hormone) at a dose of 0.2 μg / kg for pre-ripening and artificial induction; HCG (chorionic gonadotropin) was used to promote the growth of the broodstock gonads. Reach the V stage, and make it ovulate and ejaculate; artificially induce la...
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