Nutrient composition as well as preparation method and application thereof
A technology for nutritional compositions and mixtures, applied in the field of compound nutritional compositions and their preparation, can solve problems such as unfavorable internal energy, nutritional balance and function improvement, single nutrition, etc., to prevent colon cancer, enhance digestive function, and protect digestive tract. Effect
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Embodiment 1
[0057] Fucoidan with a molecular weight above 100,000 Daltons is prepared according to the following method:
[0058] 1) Mechanically grind fresh seaweed into a slurry mixture;
[0059] 2) Add 875 kg of water to 125 kg of the slurry mixture obtained in step 1), and stir at 65°C for 3 hours;
[0060] 3) Ultrafiltration of the mixed solution obtained in step 2) through a hollow fiber column with a molecular weight of 100,000 Daltons to intercept 100 kg of molecular weight above 100,000 Daltons;
[0061] 4) Add 150 kg of edible absolute alcohol to the 100 kg of intercepted liquid obtained in step 3) until the final alcohol concentration is 60% (V / V);
[0062] 5) The mixture obtained in step 4) was centrifuged at 3000-4000 rpm for 30 minutes, and then the resulting precipitate was freeze-dried at -50°C.
[0063] A 1% by weight solution was prepared, and 200 U / g of fucoidan pepsin was added to it, and after enzymolysis at 50°C for 4 hours, the molecular weight of fucoidan was red...
Embodiment 2
[0067]
[0068] The method of obtaining raw material macromolecular fucoidan:
[0069] 1) Mechanically grind fresh seaweed into a slurry mixture;
[0070] 2) Add 875 kg of water to 125 kg of the slurry mixture obtained in step 1), and stir at 65°C for 3 hours;
[0071] 3) Ultrafiltration of the mixed solution obtained in step 2) through a hollow fiber column with a molecular weight of 100,000 Daltons to intercept 100 kg of molecular weight above 100,000 Daltons;
[0072] 4) Add 150 kg of edible absolute alcohol to the 100 kg of intercepted liquid obtained in step 3) until the final alcohol concentration is 60% (V / V);
[0073] 5) The mixture obtained in step 4) was centrifuged at 3000-4000 rpm for 30 minutes, and then the resulting precipitate was freeze-dried at -50°C.
[0074] The raw material water-soluble seaweed fiber acquisition method:
[0075] 1) Mechanically mince fresh seaweed into a slurry mixture to achieve physical wall breaking;
[0076] 2) Add 2 kg of cell...
Embodiment 3
[0087]
[0088]
[0089] The method of obtaining raw material macromolecular fucoidan:
[0090] 1) Mechanically grind fresh seaweed into a slurry mixture;
[0091] 2) Add 900 kg of water to 100 kg of the slurry mixture obtained in step 1), and stir at 65°C for 3 hours;
[0092] 3) Ultrafiltration of the mixed solution obtained in step 2) through a hollow fiber column with a molecular weight of 100,000 Daltons to intercept 120 kg of molecular weight above 100,000 Daltons;
[0093] 4) Add 180 kg of edible absolute alcohol to the 120 kg of intercepted liquid obtained in step 3) until the final alcohol concentration is 60% (V / V);
[0094] 5) The mixture obtained in step 4) was centrifuged at 3000-4000 rpm for 30 minutes, and then the resulting precipitate was freeze-dried at -50°C.
[0095] The raw material water-soluble seaweed fiber acquisition method:
[0096] 1) Mechanically mince fresh seaweed into a slurry mixture to achieve physical wall breaking;
[0097] 2) Add ...
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