Method for extracting crude salamander oil from giant salamander tail fat by using papain

A technology of papain and fat extraction, which is applied in the direction of fat production, fat oil/fat production, etc., can solve the problem of low extraction rate of giant salamander oil, improve product added value and resource utilization, mild reaction conditions, and wide range of process parameters Effect

Active Publication Date: 2014-03-26
张家界金鲵生物产品开发有限公司
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AI Technical Summary

Problems solved by technology

The extraction rate of giant salamander oil is low, only 63.9% on average

Method used

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  • Method for extracting crude salamander oil from giant salamander tail fat by using papain

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0014] (1) First, separate the fat of the giant salamander tail from the skin to obtain the fat of the giant salamander tail, weigh 100g of the fat of the giant salamander tail, and then stir it into a minced shape;

[0015] (2) Add 100g of water to the minced giant salamander tail fat and mix, adjust the pH to 6.7 with HCl and NaoH, 0.3g of papain, shake well and heat up to 55°C, stir and oscillate for 1 hour.

[0016] (3) The enzymatic hydrolysis solution obtained in (2) was centrifuged at 4000r / min for 10 minutes, and then divided into three layers after centrifugation, the upper layer was clear light yellow salamander oil, the middle layer was hydrolyzed components, and the lower layer was hydrolyzed rich in polypeptides and amino acids. Liquid protein hydrolyzate, absorb the upper layer to obtain crude salamander oil 98.53g.

[0017] The extraction rate of crude salamander oil was 98.53%. The analysis results of its physical and chemical properties are shown in crude sala...

Embodiment 2

[0019] (1) First, separate the fat from the tail of the giant salamander to obtain the fat from the tail of the giant salamander, weigh 100g of the fat from the tail of the giant salamander, and stir it into a minced shape

[0020] (2) Add 100g of water to the minced giant salamander tail fat and mix it with HCl and NaoH to adjust the pH to 6.5. The weight of the subcutaneous fat of the giant salamander tail is used as the standard, add 0.1g of papain, shake well and heat up to 55°C, stir Shock reaction 1.5h.

[0021] (3) The enzymatic hydrolysis solution obtained in (2) was centrifuged at 5000r / min for 10min, and then divided into three layers after centrifugation, the upper layer was clarified light yellow salamander oil, the middle layer was hydrolyzed components, and the lower layer was hydrolyzed rich in polypeptides and amino acids Liquid protein hydrolyzate, absorb the upper layer to obtain crude salamander oil 98.4g.

[0022] The extraction rate of crude salamander oi...

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Abstract

The invention provides a method for extracting crude salamander oil from giant salamander tail fat by using papain. The method comprises the following steps: separating tail subcutaneous fat from the skin of a giant salamander to obtain the giant salamander tail fat, then stirring the giant salamander tail fat into paste, blending the giant salamander tail fat and water at the ratio of 1:0.5-1:2.0, regulating the pH value of a material liquid to 6.0-7.0, adding the papain of which the weight is 0.1-0.5% of that of the giant salamander tail fat, shaking well, heating to 40-60 DEG C, stirring and shocking for reaction for 0.5-2.0h, centrifuging the obtained enzymatic hydrolysate for 5-15min by a centrifugal machine at 4000-5000r / min, acquiring three layers of the mixture after centrifuging, and absorbing the upper layer to obtain the crude salamander oil. According to the method provided by the invention, the constraint of protein on the fat is removed, meanwhile, the protein formed by combining emulsion and the fat in the process of enzymolysis is also degraded, and the reaction condition is mild, so that effective ingredients in the salamander oil are enabled to be stable. The reaction operation is simple, the extraction ratio of the crude salamander oil and the additional valve of the product are increased, the cost is low, and the method is environment-friendly and can be widely applied to industrial production.

Description

technical field [0001] The invention relates to the technical field of animal oil extraction and processing, in particular to a method for extracting crude salamander oil from giant salamander tail fat by using papain. Background technique [0002] The Chinese giant salamander belongs to Amphibians, Cauracea, and Cryptobranchidae. It is a second-class national key protected wild animal. The Chinese giant salamander is also a traditional precious medicinal animal. ". With the maturity and promotion of large-scale domestication technology of giant salamander, the giant salamander industry has gradually become the most dynamic emerging industry for adjusting the agricultural industrial structure and increasing farmers' income in the origin area. The domestic giant salamander industry is developing rapidly, and the scale of giant salamander breeding is increasing day by day. At the end of 2012, the national giant salamander inventory was about 5 million. At present, giant sala...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C11B1/00C11B1/02
Inventor 罗庆华王苗苗吴沛霖王海磊
Owner 张家界金鲵生物产品开发有限公司
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