A method for biotransformation of glucose to generate pinoresinol and pinoresinol diglucoside by using Phomopsis
A technology of pinoresinol diglucoside and Phomopsis, which is applied in the field of microbiology and can solve the problems of organic toxicity, incorporation of intermediate impurities in products, consumption of large plants and the like
Inactive Publication Date: 2016-01-13
师俊玲
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The existing methods to obtain these two components mainly include two types, one is to extract from plants, this method needs to consume a large number of plants, and requires complex extraction and purification processes to obtain products with higher yield and purity The other method is semi-chemical synthesis, which needs to go through a relatively complicated chemical synthesis process route, using a variety of organic chemical raw materials, the whole preparation process has certain organic toxicity, and the resulting product is prone to be mixed with intermediate impurities, which affects the pharmaceutical properties of the product
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[0014] 1.1 Materials:
[0015] Bacterial strain Phomopsis XP-1; PDG standard product, purchased from China National Institute for the Control of Pharmaceutical and Biological Products, pinoresinol standard product, purchased from Sigma; acetonitrile (chromatographically pure), methanol (chromatographically pure), purchased from sigma; agar (B.R ), glucose (A.R), 95% ethanol (A.R), etc. were purchased from Shaanxi Xilong Chemical Industry.
[0016] 1.2 Method
[0017] 1.2.1 Preparation of freeze-dried bacteria: Inoculate the slant of the preserved Phomopsis XP-1 on the PDA plate medium, cultivate and activate at 28°C for 5 days; use a sterile puncher with a diameter of 1cm Punch holes to collect seeds, insert 4-6 pieces into 250mL shake flasks filled with 100mL PDB medium, and culture them on a shaker at 28°C and 180r / min for 5 days as seed solution. Then insert 10% inoculum into the new PDB medium, fill the volume of 100mL / 250mL, and cultivate it on a shaker at 28°C and 180r...
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The invention discloses a method for generating pinoresinol and pinoresinol diglucoside through bioconversion of glucose by utilizing phomopsis. The method comprises the following steps: 1) preparing phomopsis into a frozen-dry thallus; 2) adding the frozen-dry thallus into sterilized water containing glucose, and shaking the flask containing the sterilized water for bioconversion reaction; 3) after conversion cultivation, carrying out centrifugation to remove the thallus, conducting vacuum concentration on the converted liquid, adding alcohol for alcohol precipitation, staying over, filtering out polyose and other impurities, and evaporating to obtain the dry pinoresinol diglucoside. According to the method, glucose serves as the only substrate, and pinoresinol and pinoresinol diglucoside can be generated under simple reaction conditions.
Description
technical field [0001] The invention relates to a method for converting glucose by using biotechnology, which belongs to the field of microbiology. Background technique [0002] Pinoresinol and pinoresinol diglucoside are lignan compounds with various physiological activities such as anti-hypertensive, anti-cancer, and anti-oxidation, and they are only found in plants so far. The existing methods to obtain these two components mainly include two types, one is to extract from plants, this method needs to consume a large number of plants, and requires complex extraction and purification processes to obtain products with higher yield and purity The other method is semi-chemical synthesis, which needs to go through a relatively complicated chemical synthesis process route, using a variety of organic chemical raw materials, the whole preparation process has certain organic toxicity, and the resulting product is prone to be mixed with intermediate impurities, which affects the pha...
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IPC IPC(8): C12P17/18C12P19/44C12R1/645
Inventor 师俊玲高振红
Owner 师俊玲