Thin-layer chromatography detection method for pharmaceutical composition

Abstract

The invention provides a thin-layer chromatography detection method for a pharmaceutical composition. The pharmaceutical composition contains hypericum perforatum or / and acanthopanax root and is subjected to extraction, purification, enrichment, dissolving and other treatment to obtain a sample solution, the sample solution is dropped on a thin-layer silica gel plate and then is subjected to developing with a mixed solution of ethyl acetate, formic acid and water in a volume ratio of (7-18):(1-3):1 or a mixed solution of methylbenzene, ethyl acetate and methyl alcohol in a volume ratio of (4-8):(1-8):1 as a developing solvent, and then, the sample solution is viewed under an ultraviolet lamp. The method can be used for effectively separating active ingredients of the hypericum perforatum or / and the acanthopanax root in the pharmaceutical composition, the target spot location is clear, the detection result is accurate and effective without interference, the operation is simple and the detection cost is low.

Description

technical field [0001] The invention relates to the technical field of medicine, in particular to a thin-layer chromatography detection method of a pharmaceutical composition. Background technique [0002] Thin-layer chromatography is to apply a suitable adsorbent or carrier on a glass plate, and after spotting the treated sample, develop it with a developer and develop color, and compare it with the spots of the reference substance, so as to perform qualitative detection or content analysis. The main principle of the determination method is to use the different adsorption and dissolution capabilities of each component in the mixture and the adsorbent and developing agent to achieve the purpose of separation between different components. Thin-layer chromatography is one of the main means of traditional Chinese medicine detection, quality research and control of traditional Chinese medicine preparations, and is of great significance to the quality identification of traditiona...

AI Technical Summary

Problems solved by technology

However, the applicant learned through experiments that there are many problems in using this pharmacopoeia method to detect the medicinal materials of Acanthopanax senticosus, Hypericum perforatum and the pharmaceutical composition containing Acanthopanax and Hypericum perforatum: 1) The pigment interference is serious, because the pigment is in color development Brown-red under the conditions of solvent and ultraviolet light, which caused the target spots to be inconspicuous or difficult to distinguish; 2) The obtained chromatographic bands had obvious tailing, and the separation of spots was poor; 3) It was not suitable for the detection of substances containing Acanthopanax and Hypericum perforatum The pharmaceutical composition cannot effectively separate the active ingredients that need to be detected in the sample of the pharmaceutical composition, and the separation degree of the target spots of the obtained samples is poor, so it is difficult to effectively locate and detect the active ingredients

Images