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Extraction purification method and detection method of tiliroside in loropetalum chinense

A technology of purification method and detection method, which is applied in the field of extraction, purification and detection of silver linden glucoside in loropetalum, achieving the effects of strong separation ability, excellent product quality and less sample loss

Inactive Publication Date: 2014-04-02
JIANGXI HERBFINE HI TECH
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  • Abstract
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Problems solved by technology

At the same time, its detection method has been established, but this detection method has not been reported in the literature.

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  • Extraction purification method and detection method of tiliroside in loropetalum chinense
  • Extraction purification method and detection method of tiliroside in loropetalum chinense
  • Extraction purification method and detection method of tiliroside in loropetalum chinense

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[0024] Example 2:

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[0026] Embodiment 3:

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[0028] Embodiment 4:

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Abstract

The invention relates to an extraction purification method and a detection method of tiliroside in loropetalum chinense. The extraction purification method comprises the following steps: taking loropetalum chinense medicinal powder, performing reflux with 60% ethanol, separating and purifying the extract by macroporous resin, eluting with 70% ethanol, dissolving the eluate with methanol, performing reduced pressure silica gel column chromatography, performing gradient elution with ethyl acetate / methanol, dissolving flowing component A with methanol, performing filtration, precipitation, and recrystallization repeatedly, then dissolving with ethyl acetate, performing polyamide column chromatography, eluting with ethyl acetate / methanol, repeating ODS column chromatography, performing gradient elution with methanol / water, then performing MCI-gel column chromatography, performing gradient elution with methanol / water so as to obtain tiliroside. The product is identified to be tiliroside C30H26O13 by nuclear magnetic resonance spectroscopy and mass spectrography, and the content is 96.14% as determined by the detection method of the invention.

Description

technical field [0001] The invention relates to a method for extracting and purifying silver linden in loropetalum chinensis and a detection method, in particular to a method for detecting silver linden in eucalyptus alba. Background technique [0002] Tiliroside, also known as kaempferol-3-0-β-D-(6''-0-trans-p-hydroxycinnamoyl) glucopyranoside, is a flavonoid compound with yellow needles Crystal, mp.246~248℃, molecular formula C 30 h 26 o 13 , molecular weight 594.52. Studies have shown that silver satin is widely distributed in plants, and it can be isolated from plants such as Pythias persicae, Safflower, Peach Blossom, Brazilian sweet potato leaves, hollyhock flowers, and aerial parts of Psyllium pulsatillae. The content reaches 0.018%, and the content in the leaves of P. chinensis reaches 0.13%; the content in the stems of P. safflower reaches 0.0018%, and the content in the leaves of P. safflower reaches 0.12%. It has been reported that silver satin has strong ant...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07H17/07C07H1/08G01N30/02
Inventor 饶毅冯育林魏慧珍张武岗杨世林金浩鑫吕尚
Owner JIANGXI HERBFINE HI TECH
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