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Grimmia pilifera ubiquitin carboxy-terminal hydrolases gene GpUCH and coding protein thereof

A technology of P. trichomes and ubiquitin carboxyl group, which can be applied in the field of molecular biology and can solve the problems of less plant research and the like

Inactive Publication Date: 2014-04-02
QIQIHAR UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] Most of the research on UCHs is concentrated in humans and mammals, and there are few studies on plants, and there is no relevant report on the study of this gene in bryophytes.

Method used

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  • Grimmia pilifera ubiquitin carboxy-terminal hydrolases gene GpUCH and coding protein thereof
  • Grimmia pilifera ubiquitin carboxy-terminal hydrolases gene GpUCH and coding protein thereof
  • Grimmia pilifera ubiquitin carboxy-terminal hydrolases gene GpUCH and coding protein thereof

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Experimental program
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Effect test

specific Embodiment approach 1

[0030] Specific embodiment 1: The gene sequence of the Echinophylla pachyphyllum ubiquitin carboxy-terminal hydrolase gene GpUCH according to this embodiment is shown in Seq ID No: 1 in the sequence table.

specific Embodiment approach 2

[0031] Specific embodiment 2: The difference between this embodiment and specific embodiment 1 is: the encoded protein of E. torchina ubiquitin carboxy-terminal hydrolase gene GpUCH, and the amino acid sequence of the encoded protein is shown in Seq ID No: 2 in the sequence table.

[0032] Other steps and parameters are the same as those in Embodiment 1.

[0033] Verify effect of the present invention by following test:

[0034] 1: Cloning of the GPUCH gene

[0035] 1. Using Echinacea apiculata as material, total RNA was extracted by the improved SDS method:

[0036] (1) Take 0.3g of Adenophora pachyphyllum and quickly grind it into powder in liquid nitrogen, transfer it to a 1.5mL centrifuge tube, add 750μL SDS, 350μL Tris saturated phenol, 350μL chloroform, shake for 10min, and centrifuge at 12000rpm at 4°C for 10min;

[0037] SDS formula: 2% SDS, 0.0125mol / L sodium tetraborate, 50mmol / L Tris-Cl, 200mmol / L sodium chloride, 20mmol / L EDTA;

[0038](2) Take the supernatant, ...

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Abstract

The invention discloses a grimmia pilifera ubiquitin carboxy-terminal hydrolases gene GpUCH and coding protein thereof, relates to a plant drought resistance-related cDNA sequence and a coding amino acid sequence thereof, and further relates to research on drought resistance mechanism of the gene and analysis and application of drought resistance ability of transgenic plants. Gene sequence of the grimmia pilifera ubiquitin carboxy-terminal hydrolases gene GpUCH is shown in the sequence table Seq ID No:1. The coding protein of the grimmia pilifera ubiquitin carboxy-terminal hydrolases gene GpUCH is also provided, and the amino acid sequence of the coded protein is shown in the sequence table Seq ID No:2. The invention belongs to the field of molecular biology.

Description

technical field [0001] The invention relates to a cDNA sequence related to plant drought resistance and its coded amino acid sequence, and also relates to the research on the drought resistance mechanism of the gene and the analysis and application of the drought resistance ability of transgenic plants, belonging to the field of molecular biology. Background technique [0002] In nature, the growth and development of plants are closely related to changes in the environment. There are a variety of dynamic factors in the environment, such as drought, high and low temperature, etc. These factors will have adverse effects on plants in specific environments and form abiotic stress. Drought is an important stress factor restricting plant growth. my country's arid and semi-arid areas account for about 1 / 2 of the country's land area, mainly distributed in the north and northwest regions. Droughts, especially major droughts, cause water shortages, food crises, and ecological deterio...

Claims

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Application Information

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IPC IPC(8): C12N9/50C12N15/57
CPCC12N9/485C12Y304/19012
Inventor 沙伟刘博张梅娟宋璐安洪雪
Owner QIQIHAR UNIVERSITY
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