Method for preparing chestnut protein
A protein and chestnut technology, applied in the field of chestnut protein preparation, can solve problems such as protein denaturation, and achieve the effects of maintaining activity, promoting dissolution, and mild extraction conditions
Inactive Publication Date: 2016-03-30
BEIJING FORESTRY UNIVERSITY
View PDF1 Cites 0 Cited by
- Summary
- Abstract
- Description
- Claims
- Application Information
AI Technical Summary
Problems solved by technology
The other is the subcritical water extraction method. The disadvantage of this method is that the protein extraction process easily causes protein denaturation.
Method used
the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View moreImage
Smart Image Click on the blue labels to locate them in the text.
Smart ImageViewing Examples
Examples
Experimental program
Comparison scheme
Effect test
Embodiment 1
[0037] According to the general method described above, refer to figure 1 , follow the steps below to prepare chestnut protein:
[0038] Fresh chestnuts free from mildew and insects are selected as raw materials. After peeling, slicing, and freeze-drying, they are pulverized by a pulverizer and passed through a 60-mesh sieve to make chestnut powder. Then, dissolve chestnut powder and deionized water at a ratio of 1:15 (g / mL) to obtain an aqueous solution of chestnut powder and place it in an ultrasonic breaker (JAV-IV ultrasonic cell breaker, Jining Aobo Ultrasonic Electric Co., Ltd.) , under the ultrasonic power of 400w, treat for 10min. Then add cellulase (Sigma-Aldrich, product number 22178) to the chestnut powder aqueous solution that has been ultrasonically crushed at a ratio of 100 U / g, then adjust the pH to 4.5, and transfer to a 50°C constant temperature water bath (W201B constant temperature water bath pot, Shanghai Shenshun Biotechnology Co., Ltd.) and stirred for ...
the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More PUM
Login to View More Abstract
The invention discloses a method for preparing a Chinese chestnut protein. The method comprises the following steps of carrying out supersonic wave fragmentation treatment on a Chinese chestnut powder aqueous solution, carrying out first enzymolysis treatment on the Chinese chestnut powder aqueous solution subjected to supersonic wave fragmentation treatment by cellulase, carrying out second enzymolysis treatment on the first enzymoiysis product by first alpha-amylase, carrying out alkaline extraction treatment on the second enzymolysis product, carrying out first centrifugation treatment on the alkaline extraction product, collecting a supernatant so that an alkaline extraction product solution of protein is obtained conveniently, carrying out third enzymoiysis treatment on the alkaline extraction product solution of the protein by second alpha-amylase, carrying out acid precipitation treatment on the third enzymoiysis product by an isoelectric precipitation method, orderly carrying out standing and second centrifugation treatment on the acid precipitation product so that precipitates can be collected conveniently, and washing the precipitates by water until the precipitates have a pH value of 7 so that the Chinese chestnut protein is obtained conveniently. The method can effectively improve a Chinese chestnut protein extraction ratio, has mild extraction conditions so that protein activity is not destroyed, and can prepare the Chinese chestnut protein having good protein activity.
Description
technical field [0001] The invention relates to a method for preparing chestnut protein. Background technique [0002] Chestnut contains 5% to 11% protein, which is higher than rice, and amino acids such as lysine, isoleucine, methionine, cysteine, threonine, valine, phenylalanine, tyrosine, etc. The content exceeds the standard of FAO / WHO, and lysine is the first limiting amino acid of rice, wheat, corn, and soybean, threonine is the second limiting amino acid of rice and wheat, tryptophan and methionine respectively It is the second limiting amino acid in corn and beans. It can be seen that eating chestnuts or directly eating chestnut protein can supplement the deficiency of limited amino acids in cereals and beans, and can make the nutritional components of the diet more complete. [0003] However, the method for extracting and preparing chestnut protein still needs to be improved at this stage. Contents of the invention [0004] The present invention has been accomp...
Claims
the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More Application Information
Patent Timeline
Login to View More Patent Type & Authority Patents(China)
IPC IPC(8): C12P21/06
Inventor 王丰俊吕佼王建中
Owner BEIJING FORESTRY UNIVERSITY