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Use of mycobacterium tuberculosis protein in preparing products for diagnosing latent tuberculosis infection

A tuberculosis, latent technology, applied in the field of biomedicine, can solve the problems that latent tuberculosis infection cannot be diagnosed and the positive detection rate is low

Active Publication Date: 2016-01-27
TB HEALTHCARE BIOTECHNOLOGY (GUANGDONG) CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the antigens currently targeted for serological diagnosis of Mycobacterium tuberculosis, such as antigen 5, 38KD antigen, 30 / 31KD antigen, and antigen 60, all have problems such as low positive detection rate and cross-immunity with other mycobacteria. It has certain value in monitoring, prompting recurrence, judging prognosis and general screening of high-risk groups, but it still cannot be used for the diagnosis of latent tuberculosis infection

Method used

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  • Use of mycobacterium tuberculosis protein in preparing products for diagnosing latent tuberculosis infection
  • Use of mycobacterium tuberculosis protein in preparing products for diagnosing latent tuberculosis infection
  • Use of mycobacterium tuberculosis protein in preparing products for diagnosing latent tuberculosis infection

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0072] Embodiment 1, the preparation of protein chip

[0073] (1) Preparation of Mycobacterium tuberculosis antigenic proteins Rv2693c and Rv1984c

[0074] 1. Express

[0075] The genetically engineered Saccharomyces cerevisiae uses galactose to induce overexpression, and the specific process is as follows:

[0076] Firstly, from Mycobacterium M. tuberculosis H37Rv strain (Beijing strain), the gene coding fragments of protein Rv2693c and Rv1984c were de novo cloned by PCR, and the fragments were connected to the pDONR221 vector (purchased by BPase from Invitrogen Company). From Invitrogen), transformed into Escherichia coli DH5-Alpha and amplified, the extracted vector was then replaced by LR enzyme (Invitrogen) to the modified pEGH-A vector capable of expressing GST tags (the vector is in "JianZhu, HengZhu, et al: It was disclosed in J.Virol.May2009vol.83no.105219-5231", and the public can obtain it from Guangdong Tibikang Biotechnology Co., Ltd.), then transformed into Esc...

Embodiment 2

[0135] Example 2. Application of protein chip for auxiliary diagnosis of latent pulmonary tuberculosis infection

[0136] (1) Preparation of serum samples to be tested

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Abstract

The present invention provides the application of Mycobacterium tuberculosis protein Rv2693c and / or Rv1984c in the development and / or design of products for identification, diagnosis, auxiliary diagnosis, screening and / or auxiliary screening of latent tuberculosis infection. The invention also provides a protein chip prepared by using the two kinds of Mycobacterium tuberculosis proteins. Through the protein chip prepared by the present invention, detect the levels of IgM antibodies corresponding to the two proteins in the serum of patients with latent tuberculosis infection and normal people, and jointly analyze the detection results of the corresponding antibodies to the two proteins to determine whether the subject is latent tuberculosis infection, the test results show that the specificity of the optimal working point of the protein chip assisted diagnosis of latent tuberculosis infection provided by the present invention is 80.3%, and the sensitivity is 75.6%, which are higher than the diagnosis of latent tuberculosis infection in the prior art index of.

Description

technical field [0001] The invention belongs to the field of biomedicine, and specifically relates to the use of mycobacterium tuberculosis protein in preparing products for diagnosing and / or assisting in diagnosing diseases. Background technique [0002] For centuries, tuberculosis has continued to be a global public health problem that cannot be ignored. At present, one-third of the world's population is carrying Mycobacterium tuberculosis. In 2010 alone, there were 8.8 million new tuberculosis cases and 1.45 million deaths. On average, one person died of tuberculosis in less than 22 seconds. Tuberculosis is the highest infectious disease The number one death toll. my country is one of the 22 countries with a high burden of tuberculosis in the world. The number of tuberculosis patients ranks second in the world, and the number of infected people exceeds 500 million. In 2010 alone, there were 900,000 to 1.2 million new cases, accounting for the total number of new cases in...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N33/569C07K14/35
CPCG01N33/5695G01N33/6884G01N33/6893G01N2800/12
Inventor 陶生策张先恩毕利军邓教宇张治平江何伟周盈谷晶郭书娟张鸿泰王绪德王靖方侯剑周杰杨姗姗赵斐
Owner TB HEALTHCARE BIOTECHNOLOGY (GUANGDONG) CO LTD