Unlock instant, AI-driven research and patent intelligence for your innovation.

A kind of preparation method of high-efficiency starter for fermented milk

A starter and fermented milk technology, which is applied in the field of high-efficiency starter preparation, can solve the problems affecting the stability and activity of the secondary structure of lactic acid bacteria DNA and RNA, affecting the efficiency of DNA replication, transcription and translation, and the decrease of cell membrane fluidity, so as to achieve convenient Transportation and storage, low cost, fast curd effect

Inactive Publication Date: 2017-07-14
ANHUI UNIVERSITY
View PDF2 Cites 0 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

It is generally believed that the physiological impact of the freezing process on lactic acid bacteria mainly manifests in three aspects: first, the fluidity of the cell membrane decreases, thereby affecting the metabolic activity of the cells; Affect DNA replication, transcription and translation efficiency; third, the formation of ice crystals causes physical damage to cells, etc.

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0033] A method for preparing a high-efficiency starter for fermented milk, comprising the following steps:

[0034] (1) Culture medium preparation: prepare 1 L of 2.5% and 10% skim milk culture medium with water that meets the national sanitary drinking standard, 115 oC Sterilize for 15 minutes;

[0035] (2) Seed activation and improved semi-continuous culture: add lactic acid bacteria strains (dry powder) to the medium at an inoculum of 0.01-10% (W / V), 35-43 o C culture under static culture until fully curdled milk for activation (2-10 h); the activated seeds are inserted into new medium containing 2.5% skimmed milk at 5-100%, 35-43 o C expand culture to curd (2-6 h), repeat 2 times;

[0036] (3) Addition of protective agent: do not add any protective agent other than skim milk in the sterilizing solution;

[0037] (4) Freeze vacuum drying: -20 o C prefreeze for 8-24 h, turn to -40 o C vacuum freeze-dried to dry powder (24-48 h);

[0038] (5) Sterile vacuum packaging: ...

Embodiment 2

[0041] A method for preparing a high-efficiency starter for fermented milk, comprising the following steps:

[0042] (1) Culture medium preparation: prepare 1 L of 2.5% and 10% skim milk culture medium with water that meets the national sanitary drinking standard, 115 oC Sterilize for 15 minutes;

[0043] (2) Seed activation and improved semi-continuous culture: add lactic acid bacteria strains (dry powder) to the medium at an inoculum of 0.01-10% (W / V), 35-43 o C culture under static culture until fully curdled milk for activation (2-10 h); the activated seeds are inserted into new medium containing 2.5% skimmed milk at 5-100%, 35-43 o C expand culture to curd (2-6 h), repeat 2 times;

[0044] (3) Addition of protective agent: Add protective agent at 0.0001-1% (W / V) and mix well. The ingredients and ratio of the prepared protective agent are 0.0001-0.1% glutathione, 0.01-1% trehalose, 0.1- 10% oligosaccharides, 0.1-10% glycerin;

[0045] (4) Freeze vacuum drying: -20 o C...

Embodiment 3

[0049] A method for preparing a high-efficiency starter for fermented milk, comprising the following steps:

[0050] (1) Culture medium preparation: Prepare 1 L of 5% and 10% whole milk culture medium with water that meets the national sanitary drinking standard, 115 oC Sterilize for 15 minutes;

[0051] (2) Seed activation and improved semi-continuous culture: add lactic acid bacteria strains (dry powder) to the medium at an inoculation amount of 0.01-10% (W / V), 37-41 o C culture until fully curdled milk for activation (2-8 h); the activated seeds were inserted into new medium containing 5% whole milk at 10-50%, 37-41 o C expand culture to curd (2-6 h), repeat 2 times;

[0052] (3) Addition of protective agent: do not add any protective agent other than whole milk in the sterilizing solution;

[0053] (4) Freeze vacuum drying: -20 o C prefreeze for 8-24 h, turn to -40 o C vacuum freeze-dried to dry powder (24-48 h);

[0054] (5) Sterile vacuum packaging: the dried start...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention discloses a preparation method of a leavening agent for fermented milk, which comprises medium preparation, seed activation and improved semi-continuous cultivation, protection agent addition, freeze vacuum drying and aseptic vacuum packaging processes. Through the development of improved semi-continuous culture technology and starter preparation and storage methods, the purpose of increasing the bacterial concentration and cell activity of the starter, reducing the time used for fermentation, simplifying production operations, and reducing production costs is achieved. The prepared dry powder starter contained 3.7×1011 / g viable bacteria, and the curdling time of fermented milk with an inoculation amount of 1 g / L was 1.95 h.

Description

technical field [0001] The invention relates to high-density cultivation of lactic acid bacteria, freeze-vacuum drying, packaging and preservation of a starter, that is, a high-efficiency starter preparation method. Background technique [0002] High Cell Density Cultivation / high cell densityfermentation, that is, high-density fermentation technology, is an important biotechnology that significantly increases biomass and thus production efficiency. Due to the large differences between strains, the current high density standards for different strains are different. Generally, when the cell population density of microorganisms in liquid culture is more than 10 times higher than that of conventional culture, it is called high-density culture. Different strains can use different culture methods and conditions or devices to significantly increase the density of bacteria or the concentration of metabolites in the culture medium compared with traditional culture methods, thereby...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/20A23C9/127C12R1/46C12R1/225
Inventor 童望宇卢云霞罗学才
Owner ANHUI UNIVERSITY