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Microbial complex, preparation method and encapsulation pretreatment method

A compound and microbial technology, applied in biological water/sewage treatment, chemical instruments and methods, water/sludge/sewage treatment, etc., can solve the problem of large volume of external dryer, high construction cost, small particle size of microcapsules, etc. problem, to achieve the effect of improving wastewater treatment efficiency, reducing the amount of input, and high bacterial concentration

Active Publication Date: 2020-07-17
CHONGQING TAIKE ENVIRONMENTAL PROTECTION TECH CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

But the shortcoming of this method is that a, external drier volume is bigger, and construction cost is high; B, the microcapsule of preparation is usually smaller in size, and it is difficult to disperse when drying; c, core material may remain on the surface of microcapsule; d , microorganisms are sensitive to higher temperature, resulting in lower survival rate

Method used

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  • Microbial complex, preparation method and encapsulation pretreatment method
  • Microbial complex, preparation method and encapsulation pretreatment method
  • Microbial complex, preparation method and encapsulation pretreatment method

Examples

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Embodiment 4

[0030] A preparation method of the above-mentioned microbial complex, after sterilizing the microporous starch and activated carbon powder, mix them evenly with the microbial bacterial agent, then add sterile water, and cultivate in a sterile environment, aseptic The temperature of the environment is 30-39° C., and the shaking culture is carried out at a speed of 90-130 rpm for 20-55 minutes. During the shaking culture process, generation of air bubbles should be prevented to obtain the complex.

Embodiment 5

[0032] A pre-treatment method for encapsulation of the above-mentioned microbial complex, comprising mixing the complex with sterilized sodium alginate solution and Tween 80, and shaking for 10-20 minutes to obtain a complex mixed solution; the sodium alginate solution The mass percentage concentration of Tween 80 is 2-3%, and the mass percentage concentration of Tween 80 is 0.5-1.5%.

Embodiment 6

[0034] A kind of encapsulation method of above-mentioned microbial complex, comprises the following steps:

[0035] Step 1, preparing microbial bacterial agent;

[0036] Fermentation and culture of various microorganisms used for wastewater treatment to the middle and late logarithmic phases, gentle centrifugation, concentration, and washing of the bacteria; then according to the quality of the wastewater, the bacteria of various microorganisms are compounded in corresponding proportions to obtain microbial agents (per gram of microorganisms The microorganisms contained in the bacterial agent (wet weight) are 2~5×10 10 indivual).

[0037] The selected microorganism types and proportioning relationship are known technologies, such as Micrococcus, Bacillus, Alcaligenes, Flavobacterium, yeast, etc. Those skilled in the art can select the proportioning according to the actual needs, and will not repeat them here.

[0038] Step 2: After the complex is prepared according to Exampl...

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Abstract

The invention discloses a microbial compound which comprises, by weight, 80-85% of a microbial agent, 10-12% of microporous starch and 3-10% of activated carbon powder. A preparation method of the microbial compound comprises the following steps: carrying out sterilization on microporous starch and the activated carbon powder, uniformly mixing the sterilized microporous starch and the activated carbon powder with the microbial agent, adding sterile water, and culturing in an aseptic environment. An encapsulation pretreatment method of the microbial compound comprises the following steps: mixing the compound with a sterilized sodium alginate solution and tween 80, and oscillating to obtain a compound mixed liquor. By the microbial compound and its preparation method and encapsulation pretreatment method, the prepared microbial capsule has higher bacteria concentration after culture and can provide more microbes during wastewater treatment. Thus, wastewater treatment efficiency is greatly raised. Quantity of microcapsules is minimized, and costs are saved.

Description

technical field [0001] The invention relates to microbial capsules, in particular to a microbial complex, a method for preparing the microbial complex, and a method for pre-encapsulating the microbial complex. Background technique [0002] Microbial agents currently used to treat wastewater are generally added directly. Since the microbial inoculum is very sensitive to the environmental conditions of the wastewater, and the microbial inoculum is not resistant to storage, it is necessary to prolong the storage time of the microbial inoculum. [0003] Microbial encapsulation technology refers to the technology of embedding and sealing microorganisms in a specific area with membrane materials through chemical or physical immobilization methods, and maintaining the biological activity of microorganisms. Capsules consist of a core material and a wall material wrapped around the outside. After the formation of microcapsules, the coated substances are isolated from the outside wo...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N11/14C12N11/10C12N11/04C02F3/34
Inventor 谢治明王可利
Owner CHONGQING TAIKE ENVIRONMENTAL PROTECTION TECH CO LTD