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Simple and rapid separation and purification culture method of antler xylaria

A technology for the separation and purification of Ceruleus staghorn, which is applied in the biological field, can solve the problems of short fruiting body period and limited fruiting body, and achieve the effects of low production cost, easy purification, and easy material collection

Inactive Publication Date: 2014-04-16
YUNNAN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Using this method, a large number of fruiting bodies of Xanthomonas can be cultivated in a short period of time, but the cycle of the factor body is short, and the number of fruiting bodies grown on one ant nest is limited, so it is necessary to conduct pure culture research

Method used

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Examples

Experimental program
Comparison scheme
Effect test

example 1

[0018] In the place where gallinaceous fungus grows in the wild, dig a live ant nest, put a relatively complete ant nest with a large number of termites in a black plastic bag, bring it back to the room, and place it under dark conditions; For a 40*20*25cm cuboid glass fish tank, first add 3 cm of nest soil brought back from the wild to the tank; on the 5th day, a large number of termites in the plastic bag died, and 1 / 6 of them were still alive, and a large number of gray-white bacteria grew on the nest When silking, put it lightly in the tank, cover 6cm of nest soil on it, spray water until the soil surface is drenched; after 2 days, a lot of white, thin rod-shaped, less branched carbon horn bacteria grow on the soil surface, the 6th Days, the number of branches increases, the base becomes thicker and taller, and the fruiting bodies of Xanthomonas mature;

[0019] Gently remove the mature fruiting bodies of Xanthomonas growing on the soil surface with sterilized tweezers, di...

example 2

[0021] In the place where gallinaceous fungus grows in the wild, dig a live ant nest, put a relatively complete ant nest with a large number of termites in a black plastic bag, bring it back to the room, and place it under dark conditions; For a 40*20*25cm cuboid glass fish tank, first add 5 cm of nest soil brought back from the wild to the tank; on the 7th day, a large number of termites in the plastic bag died, and 1 / 4 of them were still alive, and a large number of gray-white bacteria grew on the nest When silking, put it lightly in the tank, cover 8cm of nest soil on it, and spray water until the soil surface is drenched; after 4 days, a lot of white, thin rod-shaped, less branched carbon horn bacteria grow on the soil surface, the 8th Days, the number of branches increases, the base becomes thicker and taller, and the fruiting bodies of Xanthomonas mature;

[0022] Gently remove the mature fruiting body of Xanthomonas growing on the soil surface with sterilized tweezers, ...

example 3

[0024] In the place where gallinaceous fungus grows in the wild, dig a live ant nest, put a relatively complete ant nest with a large number of termites in a black plastic bag, bring it back to the room, and place it under dark conditions; For a 40*20*25cm cuboid glass fish tank, first add 4 cm of nest soil brought back from the wild to the tank; on the 6th day, a large number of termites in the plastic bag died, and 1 / 5 of them were still alive, and a large number of gray-white bacteria grew on the nest When silking, put it lightly in the tank, cover 7cm of nest soil on it, spray water until the soil surface is drenched; after 3 days, a lot of white, thin rod-shaped, less branched carbon horn bacteria grow on the soil surface, the 7th Days, the number of branches increases, the base becomes thicker and taller, and the fruiting bodies of Xanthomonas mature;

[0025]Gently remove the mature fruiting bodies of Xanthomonas growing on the soil surface with sterilized tweezers, dip...

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PUM

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Abstract

The invention relates to a simple and rapid separation and purification culture method of antler xylaria, which belongs to the technical field of special fungal culture. The antler xylaria is less reported and belongs to the xylaria. The xylaria fungus grows on a waste nest of white ants, and since the xylaria fungus contains antibacterial active substances, antioxidant active substances, anti-virus active substances, active substances for enhancing the immunity of the human body, active substances for sedation, and other active substances, the medical value is relatively high. Due to the special living environment, the xylaria resources are rare, the xylaria is difficult to deeply research and develop at present, the fresh and strong sporocarp is difficult to encounter or collect in the field, thereby bringing certain difficulty to the separation and pure culture of the fungus. The method is characterized in that a live ant nest is buried in the indoor earth, so that a great amount of xylaria rapidly grows, and the separation and purification culture is carried out by utilizing the cultured xylaria sporocarp. The method has the characteristics of economical efficiency, simplicity, rapidness, high success rate, rapidness in propagation and the like. By adopting the method, a novel concept and a novel method can be provided for large-scale artificial culture and sustainable development of the xylaria in the future.

Description

technical field [0001] The invention relates to a simple and rapid method for separating, purifying and cultivating C. staghorn, which belongs to the field of biotechnology, and specifically belongs to the category of indoor cultivation of special fungi. Background technique [0002] Antler antlers[ Xylaria hypoxylon (L.) Grev. ] belong to the class Faecalicetes ( Sordariomycetes ), Cycloceria ( Xylariomycetidae ), Ascomycota ( Ascomycotina ), Anthracerales ( Xylariales ), Carbonaceae ( Xylariaceae ), Cyclocerus ( Xylaria ). The subpodia are small or medium-sized, 4.5-10.5cm high, 3-6mm thick, tapering at the top, with many branches, in the shape of antlers, columnar at the base, with fine hairs, dirty white, milky white, and black in the later stage; ascus shell Black, ascus 130-170 μm × 8-10 μm, 8 ascospores, smooth, without septum, 13-17 μm × 6-8 μm. C. staghorn is a new species recorded in mainland China, and there are not many data reports, and there are v...

Claims

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Application Information

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IPC IPC(8): C12N1/14C12R1/645
Inventor 李宗菊赵昱张曦予唐萍程霞冯辽辽左奎曹玉
Owner YUNNAN UNIV
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