Application of 3'-deoxyadenosine to preparation of immunologic adjuvant for synergizing broad-spectrum vaccine

A technology of deoxyadenosine and immune adjuvant, applied in the biological field, can solve the problems of small molecules, difficulty in developing attenuated strains, virus reverse mutation, etc.

Active Publication Date: 2014-04-30
FUDAN UNIV
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  • Abstract
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  • Claims
  • Application Information

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Problems solved by technology

[0002] Vaccines are an effective means to control many severe infectious diseases. Most of the currently used vaccines are attenuated strains of related pathogens. Obvious and there is a risk of virus reverse mutation
Although the newly developed recombinant nucleic acid vaccines and synthetic peptide vaccines have high purity and strong specificity, they are small in size and weak in immunogenicity, making it difficult to induce an effective immune response in the body. Adjuvants are needed to enable them to produce Antibodies or cellular immune response sufficient to fight infection

Method used

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  • Application of 3'-deoxyadenosine to preparation of immunologic adjuvant for synergizing broad-spectrum vaccine
  • Application of 3'-deoxyadenosine to preparation of immunologic adjuvant for synergizing broad-spectrum vaccine
  • Application of 3'-deoxyadenosine to preparation of immunologic adjuvant for synergizing broad-spectrum vaccine

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Experimental program
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Embodiment Construction

[0023] 1. Evaluation of the effect of 3′-deoxyadenosine on the efficacy of rabies virus vaccine.

[0024] 1. ELISA method (enzyme-linked immunosorbent assay) to detect the concentration of anti-rabies virus antibody and related cytokines in mice after immunization

[0025] The experimental animals were divided into experimental group and control group. Taking BALB / c mice as an example, experimental group 1 was intraperitoneally injected with 0.25ml human rabies vaccine (Vero cells), and experimental group 2 was intraperitoneally injected with 50 micrograms of 3′-deoxyadenosine (dissolved in 10 microliters of DMSO) and 0.25ml Rabies vaccine (Vero cells) was used for humans, and the control group was injected with the same dose of normal saline. Mice in each group were immunized once on the 0th day, the seventh day and the fourteenth day, and the injection site and dosage were the same. Blood was collected from the tail vein of mice on days 0 (before the first injection), 7 (b...

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Abstract

The invention belongs to the field of biotechnology, and particularly relates to an immunologic adjuvant and a preparation method thereof. The invention provides a synergistic effect of innate immune synergizing molecular 3'-deoxyadenosine or a mixture of 3'-deoxyadenosine and MHC epitope peptide or covalent conjugates on vaccines and antigens. The immunologic adjuvant can effectively enhance the immune effect of various vaccines (antigens), such as rabies vaccines, Hepatitis B vaccines, tuberculosis vaccines, various antigens and the like, obviously increase the titer of protective antibodies in animal bodies and the number and the functions of immune effector cells, and simultaneously accelerate the formation of the antibodies and cells obviously. The preparation method for the immunologic adjuvant is easy to implement, and can be used for developing high-efficient and quick synergistic vaccines. Therefore, the preparation method has excellent application prospect, and will bring huge social effect and economic benefits.

Description

technical field [0001] The invention belongs to the field of biotechnology, and specifically relates to the application of 3'-deoxyadenosine and its composition in the preparation of immune adjuvants for broad-spectrum vaccine (or antigen) synergism. Background technique [0002] Vaccines are an effective means to control many severe infectious diseases. Most of the currently used vaccines are attenuated strains of related pathogens. There is a clear and present danger of the virus producing back mutations. Although the newly developed recombinant nucleic acid vaccines and synthetic peptide vaccines have high purity and strong specificity, they are small in size and weak in immunogenicity, making it difficult to induce an effective immune response in the body. Adjuvants are needed to enable them to produce Antibodies, or a cellular immune response, sufficient to fight infection. [0003] The innate immune system responds quickly to pathogens, and is usually able to recogni...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K39/39A61P37/04
Inventor 朱乃硕杨艳刘蕊白豆蕫越
Owner FUDAN UNIV
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