Unlock instant, AI-driven research and patent intelligence for your innovation.

Three-dimensional culture system for tumor cell culture

A three-dimensional culture and tumor cell technology, applied in the direction of tumor/cancer cell, cell culture support/coating, tissue cell/virus culture device, etc., can solve the problem of not being able to act on tumor stromal cells at the same time, and destroying the microenvironment of tumor tissue , In vitro cell culture technology can not perfectly simulate the in vivo environment of tumor tissue and other problems

Inactive Publication Date: 2014-04-30
赵海涛
View PDF2 Cites 6 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] In vitro cell culture technology cannot perfectly simulate the in vivo environment of tumor tissue, and destroys the microenvironment of tumor tissue. The chemotherapeutic drugs used only target tumor parenchymal cells, but cannot act on tumor stromal cells at the same time.
In cell culture, cells grow on a two-dimensional plane, while in vivo tumor cells grow in a three-dimensional system. The existing cell culture technology cannot fully simulate the in vivo growth environment and biodynamic changes of tumor tissue

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Three-dimensional culture system for tumor cell culture
  • Three-dimensional culture system for tumor cell culture

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0025] Embodiment 1, establishment of three-dimensional culture system

[0026] First prepare gel for tissue culture: add 1.5 mL of coagulant (Adittive1) containing epidermal growth factor into 12 mL of matrigel for tissue culture, which contains laminin, collagen IV, fibroblast growth factor, Tissue plasminogen activator, add slowly, mix well while adding, the mixture is light yellow and clear. Add 1.5mL indicator containing phenolphthalein (Adittive2) into the mixture, add slowly and mix well. When just added, it can be seen that the local solution is pink, and after mixing, it becomes light yellow and clear. After mixing the components, it is prepared into 15mL tissue culture gel.

[0027] Add the prepared gel into a 48-well culture plate, and add 250uL of gel to each well. Place it in a clean bench and wait for the liquid gel to become translucent.

[0028] Extract surgically resected tumor tissue samples, place the tissue in PBS solution, and separate into 0.5cm 3 siz...

Embodiment 2

[0031] Embodiment 2, detection of chemotherapeutic drug sensitivity in three-dimensional culture system

[0032] Prepare the chemotherapeutic drug to be tested by using culture medium or PBS, and keep the ratio of the volume of the medium to the volume of the chemotherapeutic drug greater than 100 according to the concentration indicated in the drug instruction.

[0033] The tumor tissue pieces were cultured in the culture system for 24h, 48h, 72h, 96h and 120h, and the cell growth was observed every day, and the cell migration distance was measured.

[0034] Collect relevant data. Statistical analysis of the effects of different drugs on tumor cell growth and migration.

[0035] Using the above-mentioned three-dimensional tissue culture technology, we studied the sensitivity of hepatocellular carcinoma tissues to chemotherapy drugs ( figure 2 ), the preliminary results showed that the culture system could reflect the sensitivity of liver cancer tissue to different chemothe...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention relates to a three-dimensional culture system for tumor cell culture. Furthermore, the invention relates to a three-dimensional culture system containing gel serving as a tumor tissue growth scaffold, a culture medium, a coagulant and a tumor tissue.

Description

technical field [0001] The invention relates to a three-dimensional culture system for tumor cell culture. Further, the present invention relates to the use of the three-dimensional culture system in the screening of tumor chemotherapy drugs. Background technique [0002] At present, surgical resection of the primary lesion is the first choice in the treatment of malignant tumors. However, some patients with malignant tumors are already in the advanced stage of the tumor and cannot be treated with surgery. Moreover, after the primary lesion is surgically resected, the recurrence of the tumor will affect the efficacy of tumor treatment and reduce the risk of malignant tumors. Survival of cancer patients. Chemotherapy is one of the important means to improve the effect of tumor treatment, improve the prognosis of patients and prolong the survival period of patients. At present, the chemotherapy treatment of malignant tumors mainly adopts the systemic application of chemother...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Applications(China)
IPC IPC(8): C12M3/00C12N5/09
CPCC12M21/08C12M23/12C12M23/20C12M25/14C12N5/0693C12N2503/02C12N2513/00C12N2533/54
Inventor 赵海涛李光兵
Owner 赵海涛