Copper-loaded attapulgite clay, preparation method and use thereof

A technology of attapulgite and copper-loaded, applied in the field of copper-loaded attapulgite and its preparation, can solve the problems of shortening the course of diarrhea, achieve the effect of protecting intestinal mucosa, simple preparation method, and inhibiting the proliferation of pathogenic bacteria

Inactive Publication Date: 2014-05-07
NANJING AGRICULTURAL UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] At present, although the treatment of bacterial diarrhea with attapulgite alone has a certain effect, it is generally used in combination with other antibacterial drugs or probiotics to enhance the therapeutic effect and shorten the course of diarrhea.

Method used

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  • Copper-loaded attapulgite clay, preparation method and use thereof
  • Copper-loaded attapulgite clay, preparation method and use thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0028] Embodiment 1: Preparation of copper-loaded attapulgite

[0029] Bake the attapulgite in a muffle furnace at 350°C for 2-3 hours. Take 10g of attapulgite and add it to 200mL of copper nitrate solution, shake in a constant temperature oscillator at 200r / min to carry out ion exchange reaction. After the prepared pulp was left to stand and centrifuged, the supernatant was discarded, and washed several times with deionized water until the filtrate was dripped with 1mol / L NaOH solution without blue precipitate, that is, the excess copper ions were completely washed away. Dried in a drying oven at 105°C to constant weight, ground, and passed through a 200-mesh sieve to obtain copper-loaded attapulgite.

Embodiment 2

[0030] Embodiment 2: Determination of copper ion content in copper-loaded attapulgite

[0031] Weigh 0.1 g of the copper-loaded attapulgite prepared in Example 1, add 7 mL of 65%-68% concentrated nitric acid, 3 mL of 36%-38% concentrated hydrochloric acid, and 3 mL of 30% hydrogen peroxide. Microwave digestion in a microwave digestion apparatus for 30 min under certain conditions. All the digested solution after digestion was transferred to a 25mL volumetric flask, and the volume was adjusted to 25mL with ultrapure water.

[0032] Take single-element copper solution standard (concentration 1000 μg / mL) to prepare different concentrations of standard copper elemental solutions 0, 50, 100, 150, 200, 250 mg / L, use inductively coupled plasma emission spectrometer for detection, and draw a standard curve. At the same time, the sample digestion solution was measured, and the copper ion content in the copper-loaded attapulgite was calculated. Orthogonal experimental design was used ...

Embodiment 3

[0035] Example 3: In vitro bactericidal test of copper-loaded attapulgite on pathogenic Salmonella

[0036] Prepare 12 groups of 50mL pathogenic Salmonella bacteria liquid with sterile normal saline (the concentration of the bacteria liquid is about 1.0×10 7 CFU / mL), the copper-loaded attapulgite and the original attapulgite prepared by the method of Example 1 were added to the bacterial solution, and the tests were carried out in groups as shown in Table 1. A total of 12 groups were: adding 26.9mg / g copper-loaded attapulgite Soil component 3 groups, the amount of copper-loaded attapulgite is 5, 10, 20mg respectively; add 44.5mg / g copper-loaded attapulgite component 3 groups, the amount of copper-loaded attapulgite is 5, 10, 20mg respectively; add 51.3mg 3 groups of copper-loaded attapulgite components per g, the copper-loaded attapulgite amounts were 5, 10, and 20 mg respectively; 3 groups of attapulgite components were added, and the attapulgite amounts were 5, 10, and 20 mg...

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Abstract

The invention discloses a copper-loaded attapulgite clay. The copper-loaded attapulgite clay is an attapulgite clay containing exchangeable copper ions. The content of the copper ions in the copper-loaded attapulgite clay is 15-55 mg/g. The invention also discloses a preparation method and use of the copper-loaded attapulgite clay. The preparation method of the copper-loaded attapulgite clay disclosed by the invention is simple; the copper-loaded attapulgite clay has the characteristics of strong adsorption and sterilization effects and safety in use, and bacteria are unlikely to generate drug resistance; as a feed additive, the copper-loaded attapulgite clay is capable of effectively adsorbing pathogenic salmonella, killing salmonella, achieving the sterilizing rate of 50%-100%, effectively treating diarrhea caused by salmonella, reducing the mortality rate caused by diarrhea, achieving the effective rate of 100%, inhibiting proliferation of pathogenic bacteria in animal intestines, protecting intestinal mucosa, reducing the diarrhea rate of animals and enhancing the fertility.

Description

technical field [0001] The invention relates to a copper-loaded attapulgite and its preparation method and application, in particular to a copper-loaded attapulgite capable of adsorbing and killing pathogenic Salmonella, as well as its preparation method and application. Background technique [0002] For the prevention and treatment of bacterial gastrointestinal diseases in animals, antibiotics and other antibacterial drugs are usually used clinically. However, long-term use of antibiotics and antibacterial drugs can cause bacterial resistance, transfer of drug-resistant genes, and drug residues in products. Harmful to human health, so there is an urgent need to develop new antibacterial materials. [0003] Attapulgite is a hydrous magnesium aluminum silicate mineral, the main component is palygorskite, which belongs to the sepiolite group. Attapulgite has good ion exchange, physical adsorption, hydration and thermal stability. Adding an appropriate amount of attapulgite t...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K33/34A61P1/12A61P31/04A23K1/175
CPCY02A50/30
Inventor 姚大伟杨德吉侯羽浓许家荣高新菊丁阳
Owner NANJING AGRICULTURAL UNIVERSITY
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