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A deep sedimental source of the deep -sea deposition of tea sources of tea disease sources B5 B5

A technology of Bacillus subtilis and deep-sea sediments, applied in the directions of bacteria, microorganisms, fungicides, etc., can solve the problems of environmental pollution, quality degradation, tea production reduction, etc., and achieve the effects of rapid growth, strong inhibitory effect, and high spore rate.

Active Publication Date: 2016-05-25
THIRD INST OF OCEANOGRAPHY STATE OCEANIC ADMINISTATION +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The damage caused by pests and diseases not only reduces the yield and quality of tea, but also causes irrational use of drugs in tea gardens and excessive pesticide residues, which will pollute the environment, affect the health of consumers, and seriously hinder the export of tea. The implementation of comprehensive management of pests and diseases is the development of pollution-free tea production and ensures An important part of tea quality, high yield and sustainable development
At present, Chinese tea is a big tea-producing country in the world, but it is not yet a strong tea-producing country. A large part of the reason is that the problem of chemical pesticide residues has affected the export of tea.

Method used

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  • A deep sedimental source of the deep -sea deposition of tea sources of tea disease sources B5 B5
  • A deep sedimental source of the deep -sea deposition of tea sources of tea disease sources B5 B5
  • A deep sedimental source of the deep -sea deposition of tea sources of tea disease sources B5 B5

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0026] A The isolation and identification of bacterial strains involved in the present invention

[0027] The applicant of the present invention has isolated 32 strains of bacteria from the 1584m deep-sea sediment plate in the Southwest Pacific Basin, to isolate the tea pathogenic fungi from tea leaf spots, Phomasp. ), tea anthracnose pathogenic bacteria LH30 (Colletotrichumgloeosporioides), Staphylococcus LH107 (Neofusicoccum sp.), Alternaria LH129 (Alternariasp.), Pseudomonas LH162 (Pestalotiopsissp.) were indicator bacteria, which were detected by plate confrontation method The inhibitory activity of these 32 strains of deep-sea-derived bacteria on tea pathogenic fungi was confirmed, and one strain of Bacillus subtilis B5 showed a better inhibitory effect, and its inhibitory distance to LH13 and L30 reached 15.45mm and 14.62mm, respectively.

[0028] The strain B5 was identified as Bacillus subtilis by taxonomic research and molecular biology research, and was preserved by ...

Embodiment 2

[0071] Example 2 Inhibition of Bacillus subtilis B5 to tea pathogenic fungi

[0072] Bacillus subtilis B5 was inoculated into LB medium at 37°C and cultured at 200r / min for 1 day, then diluted with sterilized water to a bacterial cell concentration of 1×106 cells / ml to prepare a B5 live bacterial suspension. The inhibitory effect of B5 on tea pathogenic fungi was determined by plate confrontation method.

[0073] Plate confrontation method: draw a vertical cross on the bottom of the plate, place a filter paper block at an equidistant distance from the center of the PDA plate, inoculate 20 μl of B5 suspension on the paper block; inoculate a 6 mm diameter indicator pathogenic bacteria cake in the center of the plate, and culture at 28 °C After 5-7 days, measure the width of the antagonistic band when a clear zone of inhibition occurs between the antagonistic bacteria and each indicator bacteria colony. The control was not inoculated with antagonistic bacteria and only inoculate...

Embodiment 3

[0074] The preparation of embodiment 3 bacillus subtilis B5 wettable powder

[0075] 1. Determination of wetting time

[0076] The wettability of the samples was determined in accordance with the national standard GB / T5451-2001 "Determination of Wettability of Pesticide Wettable Powders". The specific method is as follows:

[0077] Take 100mL ± 1mL of standard hard water, pour it into a 250mL beaker, place the beaker in a constant temperature water bath at 25°C ± 1°C, and make the liquid level flush with the water bath level. When the hard water reaches 25 °C ± 1 °C, weigh 5 g ± 0.1 g of the sample (the sample should be a representative uniform powder, and no agglomeration or agglomeration is allowed), place it on a watch glass, and put all the samples The sample is evenly poured on the liquid surface of the beaker from a position flush with the mouth of the beaker at one time, but do not disturb the liquid surface too much. Immediately record the time with a stopwatch when...

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Abstract

The invention relates to bacillus subtilis B5 coming from deep-sea sediment and having resistance to tea leaf pathogenic funguses. The accession number of the bacillus subtilis is CCTCC M 2012384. The viable organism of the bacterial strain has high antagonistic activity to common tea leaf pathogenic funguses, and can adapt various environments. Wettable powder processed from the bacillus subtilis B5 can be used for controlling a plurality of fungus diseases. A novel bacterial strain product for biological control of diseases in a tea garden is provided.

Description

technical field [0001] The invention relates to a deep-sea sediment-sourced Bacillus subtilis B5 with resistance to the pathogenicity of various tea pathogenic fungi, and the bacterium has a strong antagonistic effect on the pathogenicity of common tea pathogenic fungi. Background technique [0002] China is the hometown of tea, and Fujian is the main tea area. There are many kinds of tea tree pests and diseases, and the damage is serious. There are more than 390 kinds of tea tree pests and more than 100 kinds of tea tree diseases. The damage caused by pests and diseases not only reduces the yield and quality of tea, but also causes irrational use of drugs in tea gardens and excessive pesticide residues, which will pollute the environment, affect the health of consumers, and seriously hinder the export of tea. The implementation of comprehensive management of pests and diseases is the development of pollution-free tea production and ensures An important part of tea quality, ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/20A01N63/00A01N25/14A01P3/00C12R1/125
Inventor 汤熙翔邓钦文易志伟
Owner THIRD INST OF OCEANOGRAPHY STATE OCEANIC ADMINISTATION