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Artificial breeding method for saccostrea cucullata

A technology of artificial breeding of mitral oysters, applied in fish farming, application, climate change adaptation, etc., can solve the problems of no artificial seed cultivation, etc., and achieve the effect of saving manpower and low cost

Active Publication Date: 2014-05-21
SOUTH CHINA SEA INST OF OCEANOLOGY - CHINESE ACAD OF SCI
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Problems solved by technology

At present, the resources of this group come entirely from wild harvesting, and there is no artificial seed cultivation

Method used

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  • Artificial breeding method for saccostrea cucullata

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Embodiment 1

[0019] (a) Collection of parents: In early April 2013, 2-3 year old wild monk's hat oyster samples were collected in the intertidal zone of Dongyong, Shenzhen. After dissection, it was found that there was a half circle of pectines on the edge of the right shell of this type of oyster, and there were no spines on the surface of the right shell. Confirmed For monk's cap oysters, according to Lam & Morton (Lam, K., Morton. Morphological and mitochondrial-DNA analysis of the indo-west pacific rock oysters (Ostreidae: Saccostrea speices) [J]. Journal of Molluscan Studies, 2006, 72:235-245) and Xia Jianjun (Xia Jianjun, South China coastal oyster species identification, distribution and Hong Kong giant oyster microsatellite development and population genetics research [D]. Doctoral dissertation of South China Sea Institute of Oceanology, Chinese Academy of Sciences, Guangzhou, 2008) classification Standards were further confirmed as D-type mitral oysters using mitochondrial sequenci...

Embodiment 2

[0024] (a) Collection of parents: 2-3 year old wild mitral oyster samples were collected in the intertidal zone of Naozhou Island, Zhanjiang in early June 2013. Anatomy revealed that there was a circle of pectines on the edge of the right shell of this type of oyster, and there were spines on the surface of the right shell. Preliminary It was confirmed as B-type mitral oyster, and was further confirmed as B-type mitral oyster by using the mitochondrial sequencing method according to the classification standards of Lam & Morton (2006) and Xia Jianjun (2008). Parents.

[0025] (b) Gonadal maturation: Put the parents of mitral oysters in scallop cages, and carry out ecological maturation in the shrimp pond of Xingxing 863 base by hanging culture. Micro-inflation, using chlorella, cloudtail algae, and flat algae as biological bait, and the amount of feeding is based on the slight green in the water to ensure that the parents have enough bait. Check the survival status of the pare...

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Abstract

The invention discloses an artificial breeding method for saccostrea cucullata. According to the artificial breeding method, by means of the technical links that parents are identified by combining the form method with the molecule method, gonads are matured by combining the ecological environment with the outdoor environment, gametes are obtained by combining natural discharge with dissection, slight air inflation is conducted, no water exchange is needed, and the filial generation is bred without changing pools, healthy saccostrea cucullata larvae are successfully bred, and the solid foundation is laid for offspring production and original variety development of saccostrea cucullata and for biodiversity protection of saccostrea cucullata. The artificial breeding method overturns the habits that in an existing shellfish larva breeding process, a large amount of water needs to be replaced, the pools need to the changed and shellfish larvae need to be fed with various types of chlamydomonas at the same time, and therefore manpower, material resources and financial resources are greatly reduced, and the novel shellfish offspring breeding mode which is low in cost, simple and efficient is created.

Description

Technical field: [0001] The invention belongs to the technical field of shellfish seed breeding in marine agriculture, and in particular relates to an artificial breeding method of mitral oysters. Background technique: [0002] Saccostrea cuculata is distributed in the Indian Ocean, the Pacific Ocean and my country's South China coast (Fujian, Guangdong, Guangxi, Hainan, Hong Kong, Macau), and is distributed in the intertidal zone from rocks, beaches to mangroves. It is a subtropical and tropical type. economic species. [0003] As a main group of wild oysters along the coast of South my country, mitral oysters often live together with Kumamoto oyster Crassostrea sikamea and Portuguese oyster C. angulata, collectively called miscellaneous oysters. Due to the delicious taste of wild oysters, they are favored by consumers and have great market development prospects. Among them, almost all Portuguese oyster seed comes from artificial breeding, with an annual output of about 1....

Claims

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Application Information

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IPC IPC(8): A01K61/00
CPCY02A40/81
Inventor 张跃环肖述张扬喻子牛
Owner SOUTH CHINA SEA INST OF OCEANOLOGY - CHINESE ACAD OF SCI
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