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Maryland tobacco polysaccharide, extracting and purifying method and application thereof as antioxidant

A purification method and polysaccharide technology, applied in chemical instruments and methods, compositions for inhibiting chemical changes, etc., achieving considerable economic benefits, simple preparation process, and broad application and development prospects

Inactive Publication Date: 2014-05-21
CHINA TOBACCO HENAN IND
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, there is no research and related reports on polysaccharides from Maryland tobacco leaves

Method used

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  • Maryland tobacco polysaccharide, extracting and purifying method and application thereof as antioxidant
  • Maryland tobacco polysaccharide, extracting and purifying method and application thereof as antioxidant
  • Maryland tobacco polysaccharide, extracting and purifying method and application thereof as antioxidant

Examples

Experimental program
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Effect test

Embodiment 1

[0026] 1) Material: Maryland tobacco leaves, produced in Yichang, Hubei

[0027] 2) Process route for extracting polysaccharides from Maryland tobacco leaves by ultrasonic method (see figure 1 )

[0028] 3) Optimal method for extracting polysaccharides from Maryland tobacco leaves by ultrasonic method

[0029] A method for extracting and purifying polysaccharides from Maryland tobacco leaves, which is obtained by the following steps: adding distilled water to crushed Maryland tobacco leaves at a material-to-liquid ratio of 1:30-40 (mass-volume ratio g:ml), and heating them at 60°C and 400- 600W ultrasonic extraction for 4-8min, and then centrifuged at 4°C, the supernatant was evaporated at 70°C and then ethanolized with absolute ethanol for 48h, centrifuged to obtain the precipitate, protein was removed by Sevage method, decolorized by activated carbon, and then decolorized with anhydrous The ethanol alcohol analysis was allowed to stand for 48 hours, and the precipitate was...

Embodiment 2

[0037] A method for extracting and purifying polysaccharides from Maryland tobacco leaves, which is obtained by the following steps:

[0038] 1) After adding the crushed Maryland tobacco leaves into distilled water at a material-to-liquid ratio of 1:35 (g:ml), ultrasonically extract at 60°C and 500W for 6 minutes, then centrifuge at 4°C, take the supernatant and use it after rotary evaporation at 70°C. Set aside for 48 hours in water and ethanol alcohol analysis, centrifuge to get the precipitate, use Sevage method to remove protein, decolorize with activated carbon, then use absolute ethanol to let stand for 48 hours and centrifuge to get the precipitate, freeze-dry at 0°C to obtain crude polysaccharide;

[0039] 2) The crude polysaccharides were separated and purified by a chromatographic column to obtain refined polysaccharides; the packing of the chromatographic column (2.5×60cm) used was Sepharose CL-6B; the eluent was 0.2 mol / L NaCl. The specific operation is: take 50 mg...

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Abstract

The invention belongs to the technical field of tobacco products, and in particular relates to Maryland tobacco polysaccharide. The invention further discloses an extracting and purifying method of the Maryland tobacco polysaccharide. The method specifically comprises the following steps: after adding crushed Maryland tobacco into distilled water in a feed liquid ratio of 1:(30-40), carrying out an ultrasonic treatment for 5-7 minutes at 50-70 DEG C at the power of 500+ / -2W; then, centrifugalizing at 4+ / -2 DEG C; taking supernate for rotary evaporation, and alcohol-separating and standing; centrifugalizing to take the precipitate; removing proteins by a Sevage method; after decoloring, alcohol-separating and standing again, centrifugalizing to take the precipitate; and freezing and drying to obtain crude polysaccharide; separating and purifying the crude polysaccharide through a chromatographic column to obtain two polysaccharide components Fr-I and Fr-II. Tests verify that the polysaccharide components Fr-I and Fr-II of Maryland tobacco have better scavenging activity to OH.free radicals and DPPH.free radicals, and the polysaccharide is a good antioxidant matter which can be used as an antioxidant.

Description

technical field [0001] The invention belongs to the technical field of tobacco leaf products, and in particular relates to a Maryland tobacco leaf polysaccharide, an extraction and purification method and its application as an antioxidant. Background technique [0002] In recent years, people have successfully extracted polysaccharides from the leaves of nearly fifty kinds of plants and widely used in the development and research of functional food and medicine, such as from mulberry leaves, ginkgo leaves, tea leaves, wolfberry leaves, lotus leaves, seabuckthorn leaves, etc. . Polysaccharides extracted from plant leaves have very important biological activities. Recently, with the development of molecular biology, people have gradually realized that polysaccharides, like proteins and nucleic acids, are biological macromolecules closely related to life activities, and have functions such as lowering blood sugar, anti-tumor, anti-virus, and improving immunity. research hotsp...

Claims

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Application Information

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IPC IPC(8): C08B37/00C09K15/06
Inventor 许春平马宇平苏东赢郑坚强毛多斌杨琛琛李萌姗刘远上
Owner CHINA TOBACCO HENAN IND