Beta-glucanase containing special enzyme for growing pigs and preparation method thereof

A technology of glucanase and growing pigs, which is applied in the field of special enzymes for growing pigs and its preparation, can solve the problems of slow growth of livestock and poultry, lack of effect, high feeding cost, etc., achieve high enzymolysis rate, reduce feed cost, The effect of a wide pH range

Active Publication Date: 2014-05-21
HUNAN HONGYING BIOTECHNOLOGY CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] At present, there are many kinds of compound enzymes for feed on the market, but some have unreasonable ratios and fail to achieve the desired eff

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0040] The preparation method of embodiment 1 Chinese herbal medicine powder

[0041] A preparation method of Chinese herbal medicine powder is as follows:

[0042] Weigh 25 parts of Radix Astragali; 15 parts of Codonopsis; 12 parts of Bupleurum; Keep at ℃ for 3h, then cool down to 50℃, add mixed enzyme preparation for enzymolysis, use lactic acid to adjust the pH value to 6, enzymolysis for 3h, finally add a mixture of ethanol and propanol twice the weight of the mixed material, 11W ultrasonic extraction for 1h, filter ; The filtrate was concentrated in vacuum and then freeze-dried to obtain Chinese herbal medicine powder.

[0043] The added amount of the mixed enzyme is 8% of the total weight of the mixed material.

[0044] The weight part array of described mixed enzyme is: 12 parts of β-glucosidase, 18 parts of xylanase, 18 parts of pentosanase, 25 parts of pullulanase, 18 parts of β-amylase, 12 parts of pectinase 12 parts of acid protease, 8 parts of papain, 8 parts of...

Embodiment 2

[0046] Embodiment 2 A kind of preparation method of β-glucanase

[0047] A preparation method for β-glucanase, comprising the steps of:

[0048] (1) Expanded cultivation of liquid seeds

[0049] ①First-level seed culture: put the activated slant strains into 500 ml shake flasks with 2 rings, the medium capacity is 100 ml, the rotary shaker is 180 rpm, the culture temperature is 40°C, and the culture time is 10 hours;

[0050] ②Cultivation of secondary seeds: Inoculate 10% of primary seeds into 500 ml secondary seed shake flasks, and the cultivation conditions are the same as primary seeds;

[0051] ③Third-level seed cultivation: Inoculate the second-level seeds with 10% inoculum amount into a 5000 ml third-level seed shaker flask, the medium capacity is 1000 ml, the rotary shaker is 100 rpm, the culture temperature is 40°C, and the culture time is 12 hours ;

[0052] ④First-level seed tank cultivation: Put the third-level seeds into the first-level seed tank with a total vo...

Embodiment 3

[0061] Embodiment 3 A kind of preparation method of compound enzyme preparation for feed

[0062] A special enzyme for growing pigs, consisting of the following enzyme preparations in parts by weight:

[0063] 1 part of Chinese herbal medicine powder, 90 parts of xylanase, 30 parts of protease, 15 parts of pectinase, 12 parts of cellulase, 20 parts of β-glucanase, 12 parts of mesophilic α-amylase.

[0064] Get 90 parts of xylanase, 30 parts of protease, 12 parts of cellulase, 12 parts of medium-temperature α-amylase, 15 parts of pectinase from Hunan Hongyingxiang Biological Engineering Co., Ltd., and the obtained 1 part of Chinese herbal medicine powder, 20 parts of β-glucanase described in Example 2 were stirred evenly in a mixer at 25° C., and the stirring time was 5 minutes. Stir evenly and store in a box at room temperature or in a cold room.

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Abstract

The invention relates to a beta-glucanase containing special enzyme for growing pigs and a preparation method thereof, belonging to the technical field of feed enzyme preparations. The special enzyme for growing pigs is prepared from the following enzyme preparations in parts by weight: 0.5-2 parts of Chinese herbal medicine powder, 80-100 parts of xylanase, 20-40 parts of protease, 10-20 parts of pectinase, 10-15 parts of cellulase, 15-25 parts of beta-glucanase and 10-15 parts of medium-temperature alpha-amylase, wherein the beta-glucanase is produced from bacillus licheniformis beta-10-25, has the collection number of CCTCC No: M2013538, and is prepared by a preparation method which comprises the following steps: carrying out inclined-face strain activation and culture, so as to obtain a liquid seed; at a fermentation stage, adopting a low-temperature culture medium supplementing and batched inoculating combined method; filtrating, concentrating, fine-filtrating and drying fermentation liquor, thereby obtaining beta-glucanase with good thermal stability. According to the compound enzyme, the conversion ratio of feed can be increased, and the cost of the feed can be reduced.

Description

Technical field: [0001] The invention belongs to the technical field of enzyme preparations, and in particular relates to a special enzyme for growing pigs and a preparation method thereof. Background technique: [0002] With the development of modern enzyme technology and biotechnology, high-efficiency biologically active substances—enzyme preparations have been able to be industrially produced on a large scale and used in the feed industry. Many experiments and practical application results have shown that feed enzyme preparations are used as A feed additive can effectively improve feed utilization, promote animal growth and prevent animal diseases. Compared with antibiotics and hormones, it has excellent safety and has attracted great attention from the feed industry worldwide. [0003] Feed enzyme preparations are enzymes added to feed to improve animal digestion and utilization of feed or to improve metabolic performance in animals. Enzyme preparations that can be adde...

Claims

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Application Information

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IPC IPC(8): C12N9/42C12N9/88C12N9/26C12N9/50A23K1/165C12R1/10
CPCA23K10/14A23K50/30C12N9/2448C12Y302/01073Y02A40/70
Inventor 李洪兵张锦杰李贵骏朱永明易继云
Owner HUNAN HONGYING BIOTECHNOLOGY CO LTD
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