Regulation and control method for improving diversity of nanocarbon to soil nematodes

A soil nematode and nano-carbon technology, applied in horticultural methods, botanical equipment and methods, horticulture, etc., can solve the problems of no significant changes, long time required for metabolism, etc.

Inactive Publication Date: 2014-05-28
TIANJIN NORMAL UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Tong et al. (2007) used PCR-DGGE for the first time to evaluate the C 60 For the influence of the anaerobic microbial colony structure and function in the soil, the study found that the structure and function of the microbial community did not change significantly after the soil anaerobic microorganisms were exposed to the experiment for 180 days; Metabolism under anaerobic conditions takes a long time, so related research still needs a long-term observation process; on the other hand, C 60 The impact on the structure and function of microbial communities cannot be extrapolated to other nanoparticles and other microbial communities, and the toxicity of different nanoparticles to microorganisms is quite different
[0007] Therefore, although nano-carbon has a certain passivation effect on heavy metals, the ecological and environmental risks brought about by its restoration of heavy metals are accompanied by

Method used

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  • Regulation and control method for improving diversity of nanocarbon to soil nematodes
  • Regulation and control method for improving diversity of nanocarbon to soil nematodes
  • Regulation and control method for improving diversity of nanocarbon to soil nematodes

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0077] A method for regulating the diversity of soil nematodes with modified nanocarbons:

[0078] (1) Handling of materials:

[0079] The collected soil samples were passed through a 2 mm sieve and mixed for use; soil properties were: pH 7.44, organic matter content 4.68, total nitrogen 0.21%, total phosphorus 22.03 mg·kg -1 , the saturated water content is 0.58 mL g -1 , and the soil sample was passed through a 2 mm sieve and mixed for use;

[0080] Tall fescue (tall fescue) Festuca arundinacea L . );

[0081] The particle size of nanocarbon is 20 nm, and the specific surface area is 1.2×10 5 m 2 ·kg -1 , pH 7, modified before application to obtain KMnO 4 , H 2 SO 4 or HNO 3 Modified nanocarbon;

[0082] (2) Experimental method:

[0083] 1) The experiment used plastic flower pots with a height of 25 cm and a diameter of 12 cm. The gauze was washed and dried, and then cut into a circular fixed flower pot with a suitable size. Each container weighed 2 kg of ma...

Embodiment 2

[0087] A method for regulating the diversity of soil nematodes with modified nanocarbons:

[0088] (1) Handling of materials:

[0089] The collected soil samples were passed through a 2 mm sieve and mixed for use; soil properties were: pH 7.44, organic matter content 4.68, total nitrogen 0.21%, total phosphorus 22.03 mg·kg -1 , the saturated water content is 0.58 mL g -1 , and the soil sample was passed through a 2 mm sieve and mixed for use;

[0090] Tall fescue (tall fescue) Festuca arundinacea L . );

[0091] The particle size of nanocarbon is 70 nm and the specific surface area is 1.2×10 5 m 2 ·kg -1 , pH 7, modified before application to obtain KMnO 4 , H 2 SO 4 or HNO 3 Modified nanocarbon;

[0092] (2) Experimental method:

[0093] 1) The experiment used plastic flower pots with a height of 25 cm and a diameter of 12 cm. The gauze was washed and dried, and then cut into a circular fixed flower pot with a suitable size. Each container weighed 2 kg of mat...

Embodiment 3

[0098] A method for regulating the diversity of soil nematodes with modified nanocarbons:

[0099] (1) Handling of materials:

[0100] The collected soil samples were passed through a 2 mm sieve and mixed for use; soil properties were: pH 7.44, organic matter content 4.68, total nitrogen 0.21%, total phosphorus 22.03 mg·kg -1 , the saturated water content is 0.58 mL g -1 , and the soil sample was passed through a 2 mm sieve and mixed for use;

[0101] Tall fescue (tall fescue) Festuca arundinacea L . );

[0102] The particle size of nanocarbon is 50nm, and the specific surface area is 1.2×10 5 m 2 ·kg -1 , pH 7, modified before application to obtain KMnO 4 , H 2 SO 4 or HNO 3 Modified nanocarbon;

[0103] (2) Experimental method:

[0104] 1) The experiment used plastic flower pots with a height of 25 cm and a diameter of 12 cm. The gauze was washed and dried, and then cut into a circular fixed flower pot with a suitable size. Each container weighed 2 kg of mat...

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Abstract

The invention discloses a regulation and control method for improving diversity of nanocarbon to soil nematodes. The method comprises the steps of adopting plastic flowerpots of which the lengths are 25cm and the diameters are 2cm, cleaning and drying gauzes, fixing each gauze which is cut to a round shape with a proper size at the bottom of each flowerpot, and weighing matrix garden soil of 2kg in each container, wherein modified nanocarbon of which the weight is 1-5% that of the matrix garden soil is added into the garden soil respectively; respectively sowing festuca arundinacea seeds of 4.5g respectively; regularly quantitatively watering in the experiment period every day to meet the plant growth demands, culturing the plants in a laboratory, wherein during the cultivation period, the temperature is 25-34 DEG C, the relative humidity is 31-48%, the lighting condition is natural incident light, the experiment time is 120 days, the nematodes in the soil are separated to identify the type and number of the nematodes as the background data of the nematodes, and the type and number of the nematodes are processed after separation and identification after 4 months.

Description

technical field [0001] The invention belongs to the technical field of environmental protection, and relates to urban greening, in particular to a method for regulating the diversity of lawn soil matrix nematodes by modified nano-carbon. Background technique [0002] Carbon black is the product of the gaseous process, which is converted from the volatile components of biomass or fossil raw materials during incomplete combustion or high-temperature pyrolysis. Usually, carbon black is a porous nanomaterial with a diameter of 30-50 nm, with a large specific surface area and high active sites. [0003] In the 1980s, the annual global carbon black production was approximately 50-270 Tg (Tg=10 12 g) or so, of which about 80% is due to combustion. Most of the carbon black directly enters the soil, accumulates gradually, and becomes an important component of soil organic matter, and the other part is lifted into the atmosphere by the wind. Due to the inert nature of carbon black,...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01G1/00A01G7/00
Inventor 多立安赵树兰贺璐
Owner TIANJIN NORMAL UNIVERSITY
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