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Method for producing nuclease P1 through large-scale liquid submerged fermentation

A liquid submerged fermentation and fermentation method technology, applied in the field of microbial fermentation enzyme preparations, can solve the problems of unrealized nuclease P1 fermentation production, etc., achieve good industrial application potential, short fermentation time, and low price

Inactive Publication Date: 2014-05-28
曾化伟
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  • Abstract
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Problems solved by technology

However, domestic enterprises have not realized the fermentative production of nuclease P1, for this reason the present invention provides a method for producing the enzyme by large-scale liquid submerged fermentation

Method used

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Examples

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Effect test

example 1

[0017] This example uses self-preserved Penicillium citrinum strains as the starting strain for fermentation, which specifically includes the following steps: (1) Insert the Penicillium citrinum strains into an eggplant-shaped bottle equipped with PDA solid medium, and place it in a constant temperature incubator at 28°C (2) All 2 Penicillium citrinum solani strains were scraped into 8L primary seed medium, and divided into 4 5L Erlenmeyer flasks, and placed in a constant temperature shaker at 28°C , cultivate 2d under rotating speed 150rpm / min (3) after sterilizing and cooling 300L seed culture medium in 500L agitated fermenter, adjust the pH value to 6.5, insert 8L seeds in the seed fermenter, keep the culture temperature at 28°C, the stirring speed is 100rpm / min, the ventilation rate is 1:0.8V / V.min, the tank pressure is 0.4MPa, and the seeds are cultivated and transplanted after 35h; (4) Put 7 tons of fermentation medium into the fermenter, sterilize and cool Finally, adju...

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PUM

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Abstract

The invention provides a technology for producing nuclease by adopting penicillium citrinum strain to perform large-scale liquid submerged fermentation. The technology comprises the following steps: firstly, performing slant culture of the strain, secondly, performing strain culture on a shaker, thirdly, performing strain enlarging culture in a fermentation tank with the volume of 500 L, and fourthly, performing large-scale production in the fermentation tank. By the adoption of the fermentation technology, the fermentation production time is only 50 to 70h in a 10-ton fermentation tank, and the activity of nuclease in fermentation liquor reaches 1,180 U / ml. The technology is relatively low in cost; compared with the activity (300 U / ml) of malt sprout extracted enzyme stock solution, the enzyme activity is greatly improved.

Description

technical field [0001] The invention relates to the technical field of microbial fermentation enzyme preparations, in particular to a method for producing nucleic acid P1 by large-scale liquid submerged fermentation. technical background [0002] 5'-Nucleotides are widely used in the fields of food, agriculture and medicine. In the field of food, 5'-nucleotides are the basic components of the taste of animal and plant foods, especially 5'-Guanosine Monophosphate (GMP for short) and 5'-Inosine Monophosphate (5'-Inosine Monophosphate). , referred to as IMP), they present a unique taste of their own when they are greater than or equal to their threshold value, and are widely used as seasoning substances. The use of 5′-nucleotides in baby food as an additive can significantly improve the baby’s immunity, promote the maturity of the intestine, promote the synthesis of lipoproteins and polyunsaturated fatty acids, and reduce the occurrence of colds and diarrhea in babies, which i...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N9/22C12R1/80
CPCC12N9/22
Inventor 曾化伟乔洁饶胜其李煜林
Owner 曾化伟
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