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A detection kit for cytochrome p450 CYP3A4 SNP

A cytochrome and kit technology, applied in the determination/inspection of microorganisms, biochemical equipment and methods, etc., can solve the problem of no statistical significance of differences

Active Publication Date: 2016-03-09
北京德能安康生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Although the dosage of sufentanil in the CT / TT group tended to be lower than that in the CC group at 24 and 48 hours after analgesia, the difference between the two groups was not statistically significant

Method used

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  • A detection kit for cytochrome p450 CYP3A4 SNP
  • A detection kit for cytochrome p450 CYP3A4 SNP
  • A detection kit for cytochrome p450 CYP3A4 SNP

Examples

Experimental program
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Effect test

Embodiment 1

[0059] Fluorescence Amplification Kit and Its Application

[0060] The kit contains: fluorescent reaction tube, deionized triple distilled water, MIX reaction solution

[0061] Wherein the fluorescent reaction tube is equipped with the following probes

[0062] Taq-manFAMprobeTCCTTCTCCATGTATCA

[0063] Taq-manVICprobeCTCCTTCTCCATGTACCA

[0064] Primer

[0065] rs2242480FTGGTGAGGAGGCATTTTTGC

[0066] rs2242480R TGCAGGAGGAAATTGATGCA

[0067] and paraffin oil

[0068] Mixed packaging, the above kits are best stored at -20°C.

[0069] The preparation method of each reagent is as follows:

[0070] Probe preparation: 25μM, deionized triple distilled water as solvent.

[0071] Primer preparation: 20 μM, deionized triple distilled water as solvent.

[0072] The ratio of each reagent is as follows:

[0073] 0.1 microliter per probe

[0074] 0.8 µl per primer

[0075] Deionized triple distilled water in a 1.5ml vial

[0076] The MIX reaction solution is contained in a 1.5...

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Abstract

The invention relates to a kit for detecting SNP (Single Nucleotide Polymorphisms) of cytochrome P450 CYP3A4. The kit comprises a probe, a primer and a MIX reaction liquid, wherein the probe has the following sequences: TCCTTCTCCATGTATCA (sequence 3) and CTCCTTCTCCATGTACCA (sequence 4), and the primer has the following sequences: TGGTGAGGAGGCATTTTTGC (sequence 5) and TGCAGGAGGAAATTGATGCA (sequence 6).

Description

technical field [0001] The invention relates to the determination of gene phenotype, in particular to a detection kit for cytochrome P450CYP3A4SNP, a detection method and an application thereof. Background technique [0002] The same drug works very well for some people and not for others because of differences in their genomes. Many of these differences manifest as variations in a single base in the human genome, that is, single nucleotide polymorphisms (SNPs). Single nucleotide polymorphisms, the full name SingleNucleotidePolymorphisms, refer to the variation of a single nucleotide on the genome to form genetic markers, which are numerous in number and rich in polymorphisms. Therefore, SNP has become the third generation of genetic markers, and many phenotypic differences in the human body, susceptibility to drugs or diseases, etc. may be related to SNP. [0003] Cytochrome P450 (CYP450) is a group of enzyme proteins containing heme, which is the main drug metabolism pha...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12Q1/68
CPCC12Q1/6883C12Q2600/106C12Q2600/156
Inventor 池里群张晓静鄂文邵宏刘叔平卢新
Owner 北京德能安康生物科技有限公司