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A kind of greenhouse planting method of clematis tissue culture seedling

A tissue culture and planting method technology, applied in the fields of botanical equipment and methods, horticulture, application, etc., can solve the problems of being susceptible to diseases and insect pests, low survival rate, etc., and achieve the effect of rapid planting

Active Publication Date: 2015-09-02
厦门乐莲乐生物科技有限公司
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0004] In the prior art, it is very common to artificially plant clematis seedlings in jars, but because clematis has strict requirements on the planting environment, it is easily infected by diseases and insect pests, and the survival rate is not high, so pesticides are used on a large scale

Method used

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  • A kind of greenhouse planting method of clematis tissue culture seedling
  • A kind of greenhouse planting method of clematis tissue culture seedling

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Embodiment

[0016] The invention discloses a greenhouse planting method for clematis tissue culture seedlings, comprising the following steps

[0017] 1. Tissue culture bottle seedlings

[0018] Clematis tissue culture bottle seedlings were moved from the cultivation room to the seedling shed, and the seedlings were hardened in a greenhouse with controllable light and temperature for 20 days. The purpose of hardening is to improve the degree of lignification of seedlings, to fully develop roots and to grow strong stems, while the purpose of opening the cover is to make the seedlings better adapt to the planting environment. The light of the seedling shed is controlled below 3500Lux, the daily light time is 10 h, and the temperature is below 30 ℃.

[0019] 2. Substrate disinfection

[0020] The substrates were mixed in proportion by volume, put into the substrate sterilization tank, and covered with plastic film. Insert a spot thermometer in the center area of ​​the sterilization pool m...

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Abstract

The invention discloses a greenhouse planting method of anoectochilus roxburghii lindl tissue culturing seedlings. The greenhouse planting method comprises the following steps of matrix disinfecting: slightly-acidic matrixes which are formed by mixing peat soils, pine needle soils and river sands in a 8:1:1 volume proportion are adopted by planting matrixes, and a steam sterilizing method is adopted for carrying out disinfecting treatment before planting; planting: the anoectochilus roxburghii lindl tissue culturing seedlings are planted on the disinfected matrixes, single-plant deep planting is carried out, and the depth is subject to a situation that first stem nodes above roots are embedded in the matrixes, and second stem nodes get close to the surface layers of the matrixes; environment controlling: the planted seedlings are put in a greenhouse of which the light illumination, the temperature and the humidity can be controlled for being cultured, the light illumination is controlled under 3000 Lux, the temperature is controlled under 30 DEG C, and the humidity is maintained to be greater than 80%. According to the greenhouse planting method of the anoectochilus roxburghii lindl tissue culturing seedlings, which is disclosed by the invention, no pesticide is needed to be used by the anoectochilus roxburghii lindl planting, no pest and disease damage is generated, the plants are robust, and the survival rate is high.

Description

technical field [0001] The invention relates to the field of biotechnology, in particular to a greenhouse planting method for clematis tissue culture seedlings. Background technique [0002] Clematis (Anoectochilus roxburghii (Wall.) Lindl) is a perennial herb of the Orchidaceae family, also known as Anoectochilus roxburghii, Anoectochilus, Anoectochilus roxburghii, Black ginseng, etc. It is a traditional precious medicinal material. It has the functions of clearing away heat and detoxifying, dispelling wind and dampness, nourishing yin and moistening the lungs, and lowering blood pressure. At the same time, the clematis is small and beautiful, with beautiful leaves and golden veins, which are arranged in a net shape, which has a very high ornamental value. [0003] The natural germination rate and reproduction rate of clematis seeds are low, the growth is slow, coupled with a large number of artificial excavation, the destruction of the ecological environment, the wild cle...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01G1/00
Inventor 石斗开陈秀环陆利春赖鹭挺陈瑞凤赖鹭捷
Owner 厦门乐莲乐生物科技有限公司
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