Tissue culture method of plumeria rubra L.cv.Acutifolia

Abstract

The invention provides a tissue culture method of plumeria rubra L.cv.Acutifolia. The method comprises the following steps: (1) culturing sterilized plumeria rubra seeds to obtain plantlets with buds; (2) cutting stems with buds from the plantlets with buds to obtain explants, and performing induction culture on the explants to grow cluster bud seedlings; (3) cutting the cluster bud seedlings, and performing seedling strengthening and rooting culture until the cluster bud seedlings grow into rooting seedlings; and (4) hardening and transplanting the rooting seedlings. According to the method, a sterile rapid propagation system of propagating buds through buds of plumeria rubra L.cv.Acutifolia is established, the stems with buds of the plumeria rubra L.cv.Acutifolia are taken as the explants for performing rapid propagation, a callus stage is avoided, the offspring variation is small, and the inheritable characters are stable.

Description

technical field [0001] The invention relates to a tissue culture method, in particular to a tissue culture method of frangipani. Background technique [0002] Frangipani (Plumeria rubra L.cv.Acutifolia), also known as Burmese gardenia, egg yolk, etc., is a deciduous shrub or small tree of the family Apocynaceae, Plumeria, native to tropical regions of America such as Mexico, Venezuela and the West Indies , is now widely planted in tropical and subtropical regions of Asia. In Xishuangbanna of my country and some countries in Southeast Asia, frangipani is an important landscape tree species in areas where Buddhist temple culture is popular. It is widely planted as one of the "five trees and six flowers", so it is also called " Temple Tree" or "Tower Tree". At present, the common varieties of frangipani include yellow frangipani, red frangipani, white frangipani, pink frangipani and so on. [0003] Frangipani has a very high ornamental value. The whole tree is well-proportioned...

AI Technical Summary

Problems solved by technology

However, it uses leaves as explants, and the explants are mostly composed of differentiated cells. The regenerated plants usually need to go through the dedifferentiation process from the differentiated state to the meristem state, and the dedifferentiated cells are in the meristem state. Under certain conditions, when the callus stage is differentiated into buds or embryoids to form plants, the offspring are prone to variation