Production process for preparing astragalus polysaccharide lipidosome nano preparation

A technology of astragalus polysaccharide and production process is applied in the field of production technology for preparing astragalus polysaccharide liposome nano-preparation, and can solve the problems of difficulty in meeting market demands, low drug efficacy, low production efficiency and the like

Active Publication Date: 2014-06-18
GUANGXI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the current production process for preparing astragalus polysaccharide liposome nano-preparation has the problem of low encapsulation efficiency, so that the drug effect is low, the production efficiency is low, and it is difficult to meet the market demand

Method used

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  • Production process for preparing astragalus polysaccharide lipidosome nano preparation

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Experimental program
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Effect test

Embodiment 1

[0014] Accurately weigh analytically pure soybean lecithin (300mg) and cholesterol (75mg) according to the mass ratio of 4:1, and dissolve them in absolute ethanol (4ml) to make a lipid solution with a mass concentration of 7.5%;

[0015] Dissolve astragalus polysaccharide (100mg) with pH 7.0 phosphate buffer (25ml) and stir (55°C constant temperature, 1800r / min) to make astragalus polysaccharide solution with a mass concentration of 0.4%;

[0016] While stirring the astragalus polysaccharide solution obtained in step , slowly inject the lipid solution obtained in step at a volume ratio of 3:1, using a No. 5 syringe needle and keeping the needle below the liquid level, continue Stir for 1 hour, remove ethanol by volatilization to obtain astragalus polysaccharide liposome suspension;

[0017] Pass the astragalus polysaccharide liposome suspension obtained in step sequentially through 0.45 μm and 0.22 μm filter membranes to obtain a transparent milky liquid, which is store...

Embodiment 2

[0019] Accurately weigh analytically pure soybean lecithin (300mg) and cholesterol (150mg) according to the mass ratio of 2:1, and dissolve them in absolute ethanol (4ml) to make a lipid solution with a mass concentration of 15%;

[0020] Dissolve astragalus polysaccharide (100mg) with pH 7.5 phosphate buffer (25ml) and stir (65°C constant temperature, 1500r / min) to make astragalus polysaccharide solution with a mass concentration of 1.0%;

[0021] While stirring the astragalus polysaccharide solution obtained in step , slowly inject the lipid solution obtained in step at a volume ratio of 4:1, using a No. 5 syringe needle and keeping the needle below the liquid level, continue Stir for 1 hour, remove ethanol by volatilization to obtain astragalus polysaccharide liposome suspension;

[0022] Pass the astragalus polysaccharide liposome suspension obtained in step sequentially through 0.45 μm and 0.22 μm filter membranes to obtain a transparent milky liquid, which is store...

Embodiment 3

[0024] Accurately weigh analytically pure soybean lecithin (300mg) and cholesterol (50mg) according to the mass ratio of 6:1, and dissolve them in absolute ethanol (4ml) to make a lipid solution with a mass concentration of 10%;

[0025] Dissolve astragalus polysaccharide (100mg) with pH 7.5 phosphate buffer (25ml) and stir (45°C constant temperature, 1600r / min) to make astragalus polysaccharide solution with a mass concentration of 0.3%;

[0026] While stirring the astragalus polysaccharide solution obtained in step , slowly inject the lipid solution obtained in step at a volume ratio of 5:1, using a No. 5 syringe needle and keeping the needle below the liquid level, continue Stir for 1 hour, remove ethanol by volatilization to obtain astragalus polysaccharide liposome suspension;

[0027] Pass the astragalus polysaccharide liposome suspension obtained in step sequentially through 0.45 μm and 0.22 μm filter membranes to obtain a transparent milky liquid, which is stored...

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Abstract

The invention discloses a production process for preparing an astragalus polysaccharide lipidosome nano preparation. An ethanol injection method is adopted, and process conditions are as follows: a mass ratio of soybean lecithin to cholesterol is (2:1)-(6:1), the final concentration of an ethanol dissolving lipoid solution is 6-15%, pH value of a phosphate buffer is 6.0-7.5 and the concentration of an astragalus polysaccharide solution is 0.3-1%. The production process disclosed by the invention can remarkably improve an entrapment efficiency of the astragalus polysaccharide lipidosome nano preparation to 36.64%, a mean grain size is 243.4nm, and the grain size of the majority is less than 1000nm; the lipidosome product is relatively ideal; the production process provides a good process route for large-scale production in the future, and can effectively enhance the medicine effect of the preparation and guarantee market supply of the natural medicine high-tech preparation replacing antibiotics and chemical drug feed additives.

Description

technical field [0001] The invention belongs to the technical field of astragalus polysaccharide preparations, in particular to a production process for preparing astragalus polysaccharide liposome nano preparations. Background technique [0002] Astragalus polysaccharide liposome nano-preparation, as a high-tech natural drug preparation, can replace antibiotics and chemical drug feed additives. It can avoid the pollution of animal products caused by drug resistance and drug residues, and reduce harmful excreta, protein consumption and nitrogen pollution of livestock and poultry. However, the current production process for preparing astragalus polysaccharide liposome nano-preparation has the problem of low encapsulation efficiency, so that the drug effect is low, the production efficiency is low, and it is difficult to meet the market demand. Contents of the invention [0003] The technical problem to be solved by the present invention is to provide a production process fo...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K9/127A61K31/715A23K1/16A61P31/04
Inventor 胡庭俊彭婷韦英益韦现色钟舒红
Owner GUANGXI UNIV
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