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Application of interstitial vascular cell and mesenchymal progenitor cell in prevention or treatment of rheumatoid arthritis

A rheumatoid, vascular layer technology, applied in the field of stem cells and biomedicine, can solve the problems of unable to control disease progression or completely cure rheumatoid arthritis, lack of prevention or treatment of rheumatoid arthritis and other problems

Pending Publication Date: 2014-06-18
CELLULAR BIOMEDICINE GRP SHANGHAI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] Therefore, traditional treatment options are still far from controlling the progression of the disease or completely curing rheumatoid arthritis, thus forcing people to seek new treatments
[0007] Therefore, there is still a lack of methods for preventing or treating rheumatoid arthritis in this field, and there is an urgent need to explore novel treatment and repair strategies.

Method used

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  • Application of interstitial vascular cell and mesenchymal progenitor cell in prevention or treatment of rheumatoid arthritis
  • Application of interstitial vascular cell and mesenchymal progenitor cell in prevention or treatment of rheumatoid arthritis
  • Application of interstitial vascular cell and mesenchymal progenitor cell in prevention or treatment of rheumatoid arthritis

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0157] SVF and haMPCs in the treatment of rheumatoid arthritis (RA)

[0158] The patient is female, 45 years old, due to repeated swelling and pain in the joints of both hands for 7 years, the test results of RF 320RU / ml and anti-CCP569.5RU / ml were diagnosed as rheumatoid arthritis.

[0159] Oral non-steroidal anti-inflammatory drugs, total glucosides of paeony, and leflunomide and other drugs, the symptoms were relieved, but liver enzymes and bilirubin increased. Switched to immunosuppressant treatment, the patient could not tolerate it. SVF and haMPCs were performed after the consent of the patients.

[0160] About 35ml of fat was extracted from the lower abdomen of RA patients, and the vascular matrix part was separated from 10ml of freshly obtained adipose tissue, and the SVF was obtained after collagenase digestion, filtration, and centrifugation to remove mature adipocytes, and the surface antigen identification of the SVF was carried out. For identification results, s...

Embodiment 2

[0164] Identification of SVF and haMPCs

[0165] flow detection

[0166] Cells were collected into centrifuge tubes by enzymatic digestion, and the cell suspension was adjusted to a density of 1×10 5 / mL, 800r / min (120g), centrifuge for 5min, discard the supernatant, wash the resuspended cells with cold D-Hanks at 4°C, centrifuge the cell suspension again at 800r / min for 5min, and then discard the supernatant. Then the cells were resuspended to 1 mL with D-Hanks, 5-10 μL of antibody was added, protected from light, and placed on ice for 30 min. Rinse with D-Hanks, centrifuge, discard the supernatant, repeat the washing process 2-3 times to ensure that unbound antibodies are removed, and finally, add about 200 to 300 μL of D-Hanks to make a suspension, and use a flow cytometer to detect ( figure 2 ).

[0167] The flow cytometric detection results of SVF are shown in Table 1.

[0168] Table 1

[0169]

[0170] The expression of cell surface antigen markers on SVF was ...

Embodiment 3

[0180] haMPCs stimulation test

[0181] (1) Fresh and frozen haMPCs were cultured in complete medium and 5% FBS medium respectively, and the VEGF secreted by haMPCs was detected.

[0182] The results show( image 3 A), in the fresh haMPCs group cultured in complete medium, the concentration of VEGF decreased with the increase of LPS concentration; in the fresh haMPCs group cultured in 5% FBS medium at 200ng / ml, the concentration of VEGF was basically the same as that of the control group, 100ng / ml and 300ng / ml decreased respectively, and the concentration of VEGF in the cryopreserved haMPCs group cultured in complete medium basically changed little with the increase of LPS concentration. On the whole, the VEGF of serum culture is higher than that of complete medium.

[0183] (2) Detected the secretion of VEGF of haMPCs under hypoxia stimulation, and found that the secretion of VEGF under hypoxia stimulation was 2-3 times that of normal culture, and the secretion of 48 hours ...

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Abstract

The invention relates to an application of an interstitial vascular cell and a mesenchymal progenitor cell in the prevention or treatment of rheumatoid arthritis. A medicinal composition containing the interstitial vascular cell and the mesenchymal progenitor cell from autologous fat is applied to a necessary object in order to substantially prevent or treat rheumatoid arthritis. The mesenchymal progenitor cell has a very high cytokine secretion ability, and can restore in-vivo body damages; and the interstitial vascular cell and the mesenchymal progenitor cell have chondroblasting and ossification ability. The invention also provides the medicinal composition containing the interstitial vascular cell and the mesenchymal progenitor cell, and a method for preventing and treating the rheumatoid arthritis.

Description

technical field [0001] The invention belongs to the field of stem cells and biomedicine. Specifically, the present invention relates to the application of interstitial vascular layer cells and mesenchymal progenitor cells in preventing or treating rheumatoid arthritis. Background technique [0002] Rheumatoid arthritis (Rheumatoid Arthritis, RA) is a common chronic inflammation in the world caused by autoimmune disorders, the immune system attacks the joints, and its pathological features are chronic inflammatory hyperplasia of the joint synovium, pannus formation , cartilage and subchondral bone destruction, eventually leading to joint deformity and ankylosis. Its prevalence accounts for about 1% of the world's total adult population, and it is about 0.32% to 0.36% in my country. The average life expectancy of patients is shortened by 5 to 10 years, and at least 50% of patients lose their working ability 10 years after the onset of disease. The clinical manifestations ar...

Claims

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Application Information

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IPC IPC(8): A61K35/12A61P19/02A61P29/00A61K35/28A61K35/35
CPCA61K35/35A61K35/12A61K35/28A61P19/02A61P29/00A61P37/00
Inventor 曹卫张丽周玉洁曾晓聆赵光宇
Owner CELLULAR BIOMEDICINE GRP SHANGHAI
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