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Preparation method for hydroxypropyl low-melting-point agarose

A technology of agarose and low melting point, which is applied in the field of preparation of hydroxypropyl low melting point agarose, can solve the problems of high price, disadvantage, increased cost of biotechnological products, etc., and achieves the effects of excellent modification effect and simple reaction steps.

Inactive Publication Date: 2014-06-18
FISHERIES RES INST OF FUJIAN
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Because the quality of domestically produced agarose products is not as good as that of similar foreign products, the agarose used in domestic scientific research mainly relies on imported products from abroad, and the imported agarose products are expensive, so my country needs to invest a considerable amount of money in imports every year. This will increase the cost of my country's biotechnology products and place it at a disadvantage in international competition

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment Construction

[0009] (1) Preparation of agarose glue: In a stainless steel interlayer stirring tank, add 225 L of deionized water, heat to boiling, pour 6 kg of agarose, heat and stir to dissolve, and adjust the temperature to 80°C for later use.

[0010] (2) Derivatization reaction: Add 350g of sodium borohydride into the reaction kettle and stir at a constant speed for 15 minutes. Add 100 L of 3.75 mol / L sodium hydroxide solution and stir for 10 min. Pass cooling water into the interlayer, cool the solution to 25°C, slowly add 7.51 L of 1,2-propylene oxide, and stir thoroughly for 60 min.

[0011] (3) Product preparation: neutralize with acetic acid to pH 7.0, add 1000L of 85% isopropanol, and stir for 30 min. The solution was pumped and filtered through a plate frame to obtain a white precipitate. The white precipitate was suspended and washed with 20 L of 60% isopropanol four times. Place the white precipitate in an oven at 50°C for drying, after drying, crush and package to obtain t...

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PUM

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Abstract

The invention relates to a preparation method for hydroxypropyl low-melting-point agarose. The preparation method comprises the following operation steps: dissolving common agarose, then adding sodium borohydride, sodium hydroxide and 1,2-epoxypropane respectively to carry out derivatization reaction, and precipitating by isopropanol to obtain the hydroxypropyl low-melting-point agarose.

Description

technical field [0001] The invention relates to a preparation method of hydroxypropyl low melting point agarose. Background technique [0002] Agarose is a widely used polysaccharide substance. It has been developed since the first separation of agarose from agar in 1937. With its nearly ideal gel properties, agarose has become a biopolymer in the modern biotechnology industry. The diffusion and point kinetics move integral to the gel matrix. With the advancement of scientific research and technology, the demand for agarose is also increasing, and it has a broad market prospect. The research on agarose in foreign countries is much earlier than that in China, and a series of products have been formed, and they have occupied the main agarose market. . Because the quality of domestically produced agarose products is not as good as that of similar foreign products, the agarose used in domestic scientific research mainly relies on imported products from abroad, and the imported...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C08B37/12
Inventor 苏永昌吴成业刘淑集王茵吴钢
Owner FISHERIES RES INST OF FUJIAN