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Method for cultivating lignicolous lucid ganoderma with walnut shells as culture medium

A technology for cultivating substrates and walnut shells, applied in botany equipment and methods, cultivation, soilless cultivation, etc., can solve the problems of unfavorable growth of Ganoderma lucidum mycelium, loss of nutrients, etc., achieve less loss of nutrients, hydrolyzed tannin Reduced effect

Inactive Publication Date: 2014-06-25
福州东星生物技术有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

The problems of the method described in this patent are: 1. Because tannin is a kind of complex polyphenolic compound in plants, it can be divided into hydrolyzed tannin and condensed tannin according to its chemical structure, and they all have similar biological properties. Both have inhibitory effects on microorganisms, including inhibition on the growth of mycelium of edible fungi such as Ganoderma lucidum
2. Use the method of soaking walnut shells at room temperature for a long time (24-72 hours), the nutrients (such as polysaccharides, proteins, etc.) in the walnut shells will gradually dissolve in water, resulting in the loss of nutrients, which is not conducive to the consumption of Ganoderma lucidum, etc. growth of fungal mycelium

Method used

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  • Method for cultivating lignicolous lucid ganoderma with walnut shells as culture medium
  • Method for cultivating lignicolous lucid ganoderma with walnut shells as culture medium

Examples

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no. 1 example

[0023] A method for carrying and cultivating Ganoderma lucidum with walnut shells as a culture substrate, comprising the steps of:

[0024] Step 1: Raw material pretreatment:

[0025] First walnut shells are crushed into particles with a diameter of 2mm;

[0026] Step 2: Preliminary hydrolysis of tannins:

[0027] Soak walnut shells in warm water at 35°C for 8 hours, the mass ratio of walnut shells to water is 1:3, remove the soaking liquid after soaking;

[0028] Step 3: Prepare soybean protein liquid:

[0029] Soak soybeans with water together, the ratio of soybeans to water is 1:4, soak continuously for 11 hours at about 20°C, and crush to make soybean milk;

[0030] Step 4: Semi-solid low-temperature fermentation:

[0031] Soybean milk and walnut shells soaked in water are fully stirred and combined to make them evenly mixed, and placed in an airtight environment at about 10°C for 93 hours, wherein the mass ratio of soybean milk to walnut shells is 1:4;

[0032] Step ...

no. 2 example

[0037] A method for carrying and cultivating Ganoderma lucidum with walnut shells as a culture substrate, comprising the steps of:

[0038] Step 1: Raw material pretreatment:

[0039] First walnut shells are crushed into particles with a diameter of 3mm;

[0040] Step 2: Preliminary hydrolysis of tannins:

[0041] Soak walnut shells in warm water at 40°C for 9 hours, the mass ratio of walnut shells to water is 1:5, remove the soaking liquid after soaking;

[0042] Step 3: Prepare soybean protein liquid:

[0043] Soak soybeans with water together, the ratio of soybeans to water is 1:3, soak continuously for 10 hours at about 25°C, and crush to make soybean milk;

[0044] Step 4: Semi-solid low-temperature fermentation:

[0045] Soybean milk and walnut shells soaked in water are fully stirred and combined to make them evenly mixed, and placed in an airtight environment at about 15°C for 96 hours, wherein the mass ratio of soybean milk to walnut shells is 1:3;

[0046] Step ...

no. 3 example

[0051] A method for carrying and cultivating Ganoderma lucidum with walnut shells as a culture substrate, comprising the steps of:

[0052] Step 1: Raw material pretreatment:

[0053] First walnut shells are crushed into particles with a diameter of 5mm;

[0054] Step 2: Preliminary hydrolysis of tannins:

[0055] Soak walnut shells in warm water at 45°C for 10 hours, the mass ratio of walnut shells to water is 1:7, remove the soaking liquid after soaking;

[0056] Step 3: Prepare soybean protein liquid:

[0057] Soak soybeans with water together, the ratio of soybeans to water is 1:3, soak continuously for 10 hours at about 25°C, and crush to make soybean milk;

[0058] Step 4: Semi-solid low-temperature fermentation:

[0059] Soybean milk and walnut shells soaked in water are fully stirred and combined to make them evenly mixed, and placed in an airtight environment at about 13°C for 96 hours, wherein the mass ratio of soybean milk to walnut shells is 1:3;

[0060] Step...

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Abstract

Provided is a method for cultivating lignicolous lucid ganoderma with walnut shells as a culture medium. The walnut shells are smashed, the walnut shells are placed in warm water for soaking, after soaking, soaking liquid is removed, and hydrolysable tannin is removed preliminarily; soybeans and water are subjected to soaking together, continuous soaking is carried out at the temperature of 20 DEG C-28 DEG C for 10 h-12 h, smashing is carried out, and soybean milk is formed; and after being soaked in the soybean milk and the water, the walnut shells are subjected to full stirring and combining, even mixing is carried out, sealing is carried out at the temperature of 10 DEG C-15 DEG C for 90 h-98 h, the processed walnut shells are placed at an oven, drying is carried out, the walnut shells, cotton seed hulls, rice bran, glucose and calcium phosphate after processing are subjected to rewatering, full stirring and even bagging, after bagging, sterilization is carried out, the walnut shells are cooled to room temperature, ganoderma lucidum strains are inoculated, breeding is carried out in an incubator of 23 DEG C-28 DEG C, and the mycelial growth conditions are observed at regular intervals until lucid ganoderma buds are formed. A semisolid low-temperature fermentation technology is used for damaging the bacteriostasis of tannin, growth of lignicolous lucid ganoderma is promoted, and hydrolysable tannin is effectively lowered.

Description

【Technical field】 [0001] The invention relates to a method for carrying and cultivating ganoderma lucidum by using walnut shells as a culture substrate. 【Background technique】 [0002] The artificial cultivation of Ganoderma lucidum in my country has been developed for many years. As a culture substrate, there are bag material cultivation and segment wood cultivation. Among them, the tree-grown Ganoderma lucidum cultivated in segment wood is the closest to the natural growth conditions and is considered to be the best quality. In recent years, due to the reduction of forestry resources, There is an urgent need for alternative methods for segmented tree cultivation of ganoderma lucidum. [0003] Yunnan and other places have an ideal environment for the cultivation of woody Ganoderma lucidum, and are also rich in walnuts. The discarded walnut shells after peeling off the walnut kernels contain a large amount of cellulose, hemicellulose, and lignin. The cultivation substrate of...

Claims

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Application Information

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IPC IPC(8): A01G31/00
Inventor 林建英陈亮
Owner 福州东星生物技术有限公司
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