Cyclic immersion type bioreactor device for strawberries and use method of device

Abstract

The invention discloses a cyclic immersion type bioreactor device for tissue culture seedlings of strawberries and a use method of the device. The device comprises a gas supply device, a liquid storage device with a liquid culture medium, a first conveying device for conveying gas of the gas supply device to the liquid storage device during the immersion period of the tissue culture seedlings of strawberries, a material storage device with the tissue culture seedlings of strawberries, a second conveying device for conveying the gas of the gas supply device to the material storage device during the non immersion period of the tissue culture seedlings of strawberries, and a communicating pipeline for communicating the material storage device with the liquid storage device. According to the device, a good growth environment is provided for tissue culture of the strawberries, and a materialization environment in a container is effectively improved. The proliferation quantity, the proliferation rate and the plant height of the tissue culture seedlings of strawberries cultured by the device are all remarkably higher than those of tissue culture seedlings of strawberries cultured by a conventional culture mode.

Description

technical field [0001] The invention belongs to the field of biotechnology, and relates to a periodic submerged bioreactor device for strawberries. Background technique [0002] Strawberry is a perennial herb and one of the most important berries in the world. Because of its short size, suitable for protected cultivation, short cycle and convenient management, it has developed rapidly in my country's fruit industry. According to relevant statistics, by 2011, my country's strawberry production and planting area has reached 2 million mu, with a total output of 2 million tons and an annual export of about 100,000 tons of strawberries. It has become the world's largest strawberry production and processing exporter. [0003] At present, there are two main culture methods of strawberry: solid culture and liquid culture. Solid culture mainly reproduces by inducing callus to regenerate adventitious buds. A large number of containers need to be processed, which consumes a lot of la...

AI Technical Summary

Problems solved by technology

[0003] At present, there are two main culture methods of strawberry: solid culture and liquid culture. Solid culture mainly reproduces by inducing callus to regenerate adventitious buds. A large number of containers need to be processed, which consumes a lot of labor and leads to high production costs

Although liquid culture does not need to change the container, it has the advantages of easy renewal, convenient cleaning, convenient application of large containers, and reduced time for seedling transfer, but its disadvantages are also obvious, such as easy hypoxia, long-term immersion in liquid, and vitrification of various organs of strawberry seedlings, etc.

The above breeding methods are difficult to meet the urgent needs of my country's strawberry industry for large-scale, high-quality seedlings

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