Preparation method for romidepsin

A technology for romidepsin and a compound, which is applied in the field of preparation of romidepsin, can solve problems such as affecting the production efficiency of romidepsin, is not simple enough, and has complicated steps, and achieves low cost, reduced synthesis steps, and simple operation. Effect

Inactive Publication Date: 2014-07-02
HYBIO PHARMA
View PDF0 Cites 10 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, this method has cumbersome steps and is not easy enough. More importantly, its total liquid phase yield...

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Preparation method for romidepsin
  • Preparation method for romidepsin
  • Preparation method for romidepsin

Examples

Experimental program
Comparison scheme
Effect test

preparation example Construction

[0050] The invention discloses a preparation method of romidepsin, and those skilled in the art can refer to the content of this article and appropriately improve the process parameters to realize it. In particular, it should be pointed out that all similar replacements and modifications are obvious to those skilled in the art, and they are all considered to be included in the present invention. The method of the present invention has been described through preferred embodiments, and the relevant personnel can obviously make changes or appropriate changes and combinations to the compounds and preparation methods described herein without departing from the content, spirit and scope of the present invention to achieve and Apply the technology of the present invention.

[0051] In the specific embodiment of the present invention, all amino acids coupled with protecting groups can be obtained commercially. The protected amino acids in the present invention are purchased from Jill ...

Embodiment 1

[0055] Embodiment 1: the preparation of formula 1 compound

[0056] Weigh 2g of CTC Resin with a substitution degree of 0.5mmol / g (synthesis scale: 1mmol), add it to a solid-phase reaction column, wash it twice with DMF, and swell the resin with DMF for 30 minutes, then weigh 1.26g of 3-hydroxyl-7-( Trityl)mercapto-4-heptenoic acid was dissolved in DMF, activated by adding 0.6mL DIPEA in an ice-water bath, then added to the above-mentioned reaction column equipped with resin, reacted for 2 hours, and the reaction was completed, washed with DMF for 6 times to obtain formula 1 compound.

Embodiment 2

[0057] Embodiment 2: the preparation of formula 2 compound

[0058] Dissolve 1.01g of Fmoc-Val-OH, 0.38g of HOBt, and 0.03g of DMAP in a mixed solution of DMF and NMP with a volume ratio of 1:1, add 0.3mL of DIC to activate it under an ice-water bath, and then add it to the solid-phase reaction column in Example 1 React with the compound of formula 1, and react at room temperature for 2 hours (the end point of the reaction is determined by the ninhydrin method. If the resin is colorless and transparent, the reaction is complete, and the resin develops color, indicating that the reaction is incomplete, and another coupling reaction is required for 1 hour). The Fmoc protecting group was then removed with DBLK and washed 6 times with DMF.

[0059] Then repeat the above steps of adding coupling agent and DMAP, adding amino acid and removing Fmoc protecting group, and complete Fmoc-L-Thr-OH, Fmoc-D-Cys(Trt)-OH, Fmoc-D-Val-OH one by one Coupling of the polypeptide chain extension t...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

PUM

No PUM Login to view more

Abstract

Disclosed is a method for preparing Romidepsin. The present invention relates to the pharmaceutical synthesis. The present invention is based on a method of solid phase synthesis. First, coupling is performed between resin and carboxyl groups on 3-hydroxy-7-mercapto-4-heptenoic acid; then,coupling is performed in sequence between four pieces of amino acid on Romidepsin; then, hydroxyl groups are removed, and disulfide bonds and amido bonds are obtained by means of cyclization, so as to form Romidepsin. The purity the finally finished product is greater than 99%, the total yield is higher than 30%, and method features simple preparation, a short synthesis cycle and low cost, and helps to produce Romidepsin in a large scale.

Description

technical field [0001] The invention relates to the field of pharmaceutical synthesis, in particular to a preparation method of romidepsin. Background technique [0002] Romidepsin, the English name is Romidepsin, its chemical name is (1S,4S,7Z,10S,16E,21R)-7-ethylidene-4,21-diisopropyl-2-oxa-12, 13-dithio-5,8,20,23-tetraazabicyclo[8,7,6]triacos-16-ene-3,6,9,19,22-pentone, the molecular formula is C 24 h 36 N 4 o 6 S 2 , is a bicyclic tetrapeptide with a stable hydrophobic structure, and the unique disulfide bond in its structure is the key group for its activity. In 2009, romidepsin was approved by the US Food and Drug Administration (FDA) for the treatment of cutaneous T-cell lymphoma (CTCL). Its chemical structure is as follows: [0003] [0004] Romidepsin [0005] Romidepsin is an inhibitor of histone deacetylases (HDACs), which enters the cytoplasm through the tumor cell membrane, and the disulfide bond in the cell is reduced to a sulfhydryl group by glutath...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

Application Information

Patent Timeline
no application Login to view more
IPC IPC(8): C07K5/10C07K1/20C07K1/06C07K1/04
CPCC07K5/0808C07K5/12C07K1/06
Inventor 肖庆潘俊锋马亚平袁建成
Owner HYBIO PHARMA
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Try Eureka
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap