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Application of Signal Regulatory Protein α (shsp-1) Gene in Myocardial Infarction

A signal regulation protein, myocardial infarction technology, applied in the field of gene function and application

Active Publication Date: 2015-10-28
武汉惠康基因科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

So far, there is no international literature report on the application of SHPS-1 gene in coronary atherosclerotic heart disease

Method used

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  • Application of Signal Regulatory Protein α (shsp-1) Gene in Myocardial Infarction
  • Application of Signal Regulatory Protein α (shsp-1) Gene in Myocardial Infarction
  • Application of Signal Regulatory Protein α (shsp-1) Gene in Myocardial Infarction

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0041] [Example 1] Myocardial infarction (MI) model acquisition

[0042] 1. Grouping of experimental animals: male C57BL / 6 background WT mice, SHPS-1 knockout mice (SHPS-1-KO), heart-specific SHPS-1 transgenic mice (SHPS-1-TG) and non-transgenic mice Mice (NTG), the myocardial infarction (MI) model was induced by ligation of the left anterior descending coronary artery (LAD) of the mouse heart. They were randomly divided into 8 groups, each group: C57BL / 6 background WT mouse sham operation group (WT Sham) and MI operation group (WT MI), SHPS-1 gene knockout mouse sham operation group (SHPS-1-KO Sham ) and MI operation group (SHPS-1-KO MI), non-transgenic mouse sham operation group (NTG Sham) and MI operation group (NTG MI), heart-specific SHPS-1 transgenic mouse sham operation group (SHPS-1 -TG Sham) and MI surgery group (SHPS-1-TG MI).

[0043] 2. The MI model uses blocking the left anterior descending coronary artery (LAD) of the mouse heart to cause myocardial infarction....

Embodiment 2

[0052] [Example 2] Mouse myocardial infarction (MI) model myocardial infarction ratio, myocardial hypertrophy and fibrosis detection

[0053] 1. Collect materials

[0054] (1) Preliminary work: prepare a urine cup filled with 20mL of 10% formaldehyde in advance, and label it (mouse number, group, type of operation and date of collection). Place the petri dish filled with 10% KCl solution at the sampling site. Turn on the analytical balance, adjust to zero, and then weigh and kill the mice.

[0055] (2) Material collection: Ophthalmic curved forceps clamped the vascular pedicle below the atrial appendage, cut off the heart, and quickly placed it in 10% KCl solution. After the heart stops beating in the diastolic phase, place it on sterilized gauze, gently squeeze the fluid in the heart cavity, dip the surface fluid dry, weigh and record, put the heart into the corresponding urine cup, fix it for 48 hours and use it for pathological testing.

[0056] (3) Relevant measurement...

Embodiment 3

[0073] [Example 3] Cardiac function detection in myocardial infarction (MI) model mice

[0074] 1 Ultrasound detection of cardiac function

[0075] 1.1 Preliminary preparation

[0076] (1) Anesthesia machine preparation: first connect the oxygen cylinder and the air inlet port on the anesthesia machine, then unscrew the sealing cap of the dosing port on the anesthesia machine, quickly add isoflurane to the safety scale, and then tighten the sealing cap. Unscrew the main valve on the oxygen cylinder, adjust the knob of the flow control valve, and maintain the outlet pressure at 0.2-0.3mPa.

[0077] (2) Preparation of the mice to be tested: After the mice to be tested were quickly anesthetized with isoflurane, the hair on the left chest area was shaved, and the head of the treated mice was inserted into the anesthetic catheter sleeve, and treated with 1.5-2.0% isoflurane Alkane maintains a stable anesthesia in mice.

[0078] 1.2 Cardiac function test

[0079] The mice were p...

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Abstract

The invention discloses an application of signal regulatory protein alpha (SIPR alpha, SHPS-1) genes in coronary atherosclerotic heart diseases, belonging to the field of function and application of genes. Mice of which the SHPS-1 genes are knocked out and cardiac-specific SHPS-1 transgenic mice are adopted as experimental subjects; MI (myocardial infarction) models are formed by blocking LAD (left anterior descending coronary artery) of the hearts of the mice, and the result shows that compared with WT (wild type) control mice, the cardiac infarction proportion, cardiac hypertrophy and degree of fibrosis of the mice of which the SHPS-1 genes are knocked out, subjected to an MI operation, are obviously inhibited, and the cardiac functions of the mice are obviously improved; the cardiac infarction proportion, cardiac hypertrophy and degree of fibrosis of the cardiac-specific SHPS-1 transgenic mice are obviously increased, and the cardiac functions of the mice are obviously worsened, so that the result shows that the SHPS-1 genes can promote and worsen the occurrence and development of the coronary atherosclerotic heart diseases; SHPS-1 can be used as a medicament target for screening medicaments which are used for treating the coronary atherosclerotic heart diseases; an inhibitor of SHPS-1 can be used for preparing the medicaments for treating the coronary atherosclerotic heart diseases.

Description

technical field [0001] The invention belongs to the field of gene function and application, in particular to the function and application of a signal regulatory protein (SIRPα, SHPS-1) in coronary atherosclerotic heart disease. Background technique [0002] Cardiovascular disease is the disease that causes the highest death rate in the world and is the number one killer of human health. Ischemic heart disease is the leading cause of cardiovascular death. Coronary atherosclerotic heart disease is a disease in which a large amount of lipid deposits on the inner wall of the coronary arteries supplying the myocardium, forming plaques and thrombi that narrow or block the coronary arteries, causing myocardial ischemia, hypoxia or necrosis. Myocardial infarction (MI) is a common type of ischemic heart disease. The coronary artery is occluded and the blood flow is interrupted, resulting in partial necrosis of some myocardium due to severe and persistent ischemia. Within a few minu...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12Q1/02A61K49/00A61K45/00A61K48/00A61K39/395A61P9/10
Inventor 李红良蒋丁胜王丕晓刘小熊
Owner 武汉惠康基因科技有限公司
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