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Superparamagnetic iron oxide nano-particle-collagen-chitosan magnetic degradable nerve conduit and preparation method thereof

A technology of iron oxide nanometers and nerve conduits, which is applied in the fields of medical science and prostheses, can solve problems such as poor functional recovery of the innervation area, poor repair effect of nerve stents, and uncertain growth direction of regenerative nerves, etc., so as to improve the repair effect, Promotes the regeneration of damaged nerves and promotes secretion

Inactive Publication Date: 2014-07-09
FOURTH MILITARY MEDICAL UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0008] In order to solve the problem that the growth direction of the regenerated nerve is uncertain, the repair effect of the existing nerve support is not good, the nerve growth rate is slowed down, and the function of the innervation area is poorly restored, the present invention provides superparamagnetic iron oxide nanoparticles-collagen-chitosan Magnetic degradable nerve guide and its preparation method

Method used

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  • Superparamagnetic iron oxide nano-particle-collagen-chitosan magnetic degradable nerve conduit and preparation method thereof
  • Superparamagnetic iron oxide nano-particle-collagen-chitosan magnetic degradable nerve conduit and preparation method thereof
  • Superparamagnetic iron oxide nano-particle-collagen-chitosan magnetic degradable nerve conduit and preparation method thereof

Examples

Experimental program
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Effect test

Embodiment 1

[0030] (1) Prepare 0.1mol / L Fe respectively 3+ and Fe 2+ solution, under nitrogen, according to Fe 3+ :Fe 2+ Mix at a molar ratio of 2:1, and mechanically stir for 3 hours to make it fully mixed. Under the condition of continuous nitrogen flow, 1 mol / L NaOH solution was added dropwise to the mixed solution until the pH of the mixed solution was 9, and a black precipitate was obtained. Then the water bath was heated for 2h, and then after standing for 5h, the supernatant was removed, the generated particles were filtered under reduced pressure, washed repeatedly with distilled water and absolute ethanol, and the impurity ions on the surface of the particles were washed away, and the obtained particles were obtained at 50 vacuum-dried at ℃, ground and sieved to obtain SPIONs.

[0031] (2) Weigh type I collagen and chitosan respectively, the masses of type I collagen and chitosan are 50 mg and 50 mg respectively, and weigh 5 mg of SPIONs prepared in (1).

[0032] (3) Add 1ml...

Embodiment 2

[0037] (1) Prepare 0.1mol / L Fe respectively 3+ and Fe 2+ solution, under nitrogen, according to Fe 3+ :Fe 2+ Mix at a molar ratio of 2:1, and stir mechanically for 3.5 hours to make it fully mixed. Under the condition of continuous nitrogen flow, 1 mol / L NaOH solution was added dropwise to the mixed solution until the pH of the mixed solution was 9.5, and a black precipitate was obtained. Then the water bath was heated for 2h, and then after standing for 5h, the supernatant was removed, the generated particles were filtered under reduced pressure, washed repeatedly with distilled water and absolute ethanol, and the impurity ions on the surface of the particles were washed away, and the obtained particles were obtained at 50 vacuum-dried at ℃, ground and sieved to obtain SPIONs.

[0038] (2) Weigh type I collagen and chitosan respectively. The masses of type I collagen and chitosan are 80 mg and 20 mg respectively, and weigh 10 mg of SPIONs prepared in (1).

[0039] (3) Ad...

Embodiment 3

[0044] (1) Prepare 0.1mol / L Fe respectively 3+ and Fe 2+ solution, under nitrogen, according to Fe 3+ :Fe 2+ Mix at a molar ratio of 2:1, and mechanically stir for 4 hours to make it fully mixed. Under the condition of continuous nitrogen flow, 1 mol / L NaOH solution was added dropwise to the mixed solution until the pH of the mixed solution was 10, and a black precipitate was obtained. Then the water bath was heated for 2h, and then after standing for 5h, the supernatant was removed, the generated particles were filtered under reduced pressure, washed repeatedly with distilled water and absolute ethanol, and the impurity ions on the surface of the particles were washed away, and the obtained particles were obtained at 50 vacuum-dried at ℃, ground and sieved to obtain SPIONs.

[0045] (2) Weigh type I collagen and chitosan respectively. The masses of type I collagen and chitosan are 20 mg and 80 mg respectively, and weigh 20 mg of SPIONs prepared in (1).

[0046] (3) Add 1...

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Abstract

The invention discloses a superparamagnetic iron oxide nano-particle-collagen-chitosan magnetic degradable nerve conduit and a preparation method thereof. The preparation method comprises the steps of (1) preparing SPIONs (Superparamagnetic Iron Oxide Nano-particles); (2) preparing an SPIONs-collagen-chitosan magnetic degradable nerve conduit; and (3) carrying out disinfection treatment. With the adoption of the preparation method, in the process of preparing the superparamagnetic iron oxide nano-particle-collagen-chitosan magnetic degradable nerve conduit, as superparamagnetic iron oxide nano-particles with a certain concentration are adopted to form a magnetizable magnetic system, regenerated nerves can be guided to carry out orientated growth, the secretion of nerve nutrient substances can also be promoted, and damaged nerves can be more effectively promoted to regenerate. With the adoption of the prepared nerve conduit, the regenerated nerves can carry out orientated growth and accelerated repair to compound a myelin sheath by virtue of an internal magnetic field of the conduit and can be used for further improving the repair effect in nerve defect.

Description

technical field [0001] The invention relates to the technical field of long-segment peripheral nerve injury repair, in particular to a superparamagnetic iron oxide nanoparticle-collagen-chitosan magnetically degradable nerve guide and a preparation method thereof. Background technique [0002] Peripheral nerve defect is one of the most common clinical injuries. This type of nerve injury will cause complete loss of both sensory and motor functions in the distal limbs innervated by the affected nerves, resulting in severe disability, which will have a huge impact on the patient's work and quality of life. [0003] At present, clinically, for short-distance peripheral nerve defects, on the basis of the principle of tension-free suture, the method of direct suture of nerve stumps is often used for repair; for long-distance peripheral nerve defects, autologous nerve transplantation is often used. method to repair. However, studies have shown that: autologous nerve transplantati...

Claims

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Application Information

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IPC IPC(8): A61L27/24A61L27/20A61L27/50
Inventor 罗卓荆黄景辉刘鐘阳刘靓
Owner FOURTH MILITARY MEDICAL UNIVERSITY
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