A kind of mycorrhized dendrobium seedling tea tree imitation wild cultivation method

A technology imitating wild cultivation and cultivation methods, applied in the field of mycorrhizal Dendrobium seedlings and tea trees imitating wild cultivation, can solve the problems of insufficient medicinal value, etc.

Active Publication Date: 2016-07-06
贵州涵龙生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] The technical problem to be solved by the present invention is: aiming at the shortage of medicinal value of pure artificially planted dendrobium, a method of imitating wild planting is provided to improve the medicinal value of dendrobium and improve economic benefits

Method used

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  • A kind of mycorrhized dendrobium seedling tea tree imitation wild cultivation method

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0047] Step 1: Fermentation of mycorrhizal fungi

[0048] A. Select the fresh vegetative roots of wild dendrobium, wash off the humus and sundries on the root surface with running water; immerse the root segment in 1% calcium hypochlorite solution for 3 minutes on the ultra-clean workbench to sterilize the surface, and then rinse with sterile water Rinse 3-5 times; cut the root section into 0.5-3mm long pieces, place it on the separation medium, and cultivate it in the dark at 27°C. After culturing and purifying on the substrate, transfer it to solid fermentation medium, culture at a constant temperature of 5°C for 40 days, and punch holes at the edge of the colony to form bacterial flakes, which can be used directly;

[0049] The separation medium is: glucose 20g / L, peptone 8g / L, streptomycin 0.5g / L, ground moss dentata 25g / L, casein hydrolyzate 3g / L, agar 19g / L, pH 4;

[0050] The fermentation medium is: 120g / L potato, 180g / L glucose, 100g / L moss moss grinding, 8g / L pepton...

Embodiment 2

[0071] Step 1: Fermentation of mycorrhizal fungi

[0072] A. Select the fresh vegetative root of wild dendrobium, wash off the humus and sundries on the root surface with running water; immerse the root section in 1% calcium hypochlorite solution on the ultra-clean workbench for 7 minutes to sterilize the surface, and then wash it with sterile water Rinse 3-5 times; cut the root section into 0.5-3mm long pieces, place it on the separation medium, and cultivate it in the dark at 26°C. After culture and purification on the substrate, transfer to solid fermentation medium, and after 40 days of constant temperature cultivation at 8°C, punch holes on the edge of the colony to form bacterial flakes, which are further inserted into liquid fermentation medium as mycorrhizal fungus seed strains for amplified cultivation;

[0073] The separation medium is as follows: glucose 15g / L, peptone 13g / L, streptomycin 0.3g / L, ground moss dentata 10g / L, casein hydrolyzate 3g / L, agar 23g / L, pH 4....

Embodiment 3

[0095] Step 1: Fermentation of mycorrhizal fungi

[0096] A. Select the fresh vegetative root of wild dendrobium, wash off the humus and sundries on the root surface with running water; immerse the root segment in 1% calcium hypochlorite solution for 1-10min on the ultra-clean workbench to sterilize the surface, and then use sterile Rinse with bacterial water 3-5 times; cut the root section into 0.5-3mm long pieces, place them on the separation medium, and cultivate them in the dark at 20-30°C. After the mycelium grows into bacteria from the root section, pick the bacteria After the silk is cultured and purified on the separation medium, it is transferred to the solid fermentation medium, and after 30 days of constant temperature cultivation at 23°C, holes are punched at the edge of the colony to form bacterial flakes, which are further inserted into the liquid as mycorrhizal fungus seed strains;

[0097] The separation medium is: glucose 8g / L, peptone 4g / L, streptomycin 0.02g...

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Abstract

The invention belongs to the technical field of plantation, and concretely relates to an imitation wild cultivation method of mycorrhizated dendrobium plant tea tree. The cultivation method comprises the steps of fermentation culturing of mycorrhizal fungi, culturing of dendrobium tissue culture plants, symbiotic cultivation of dendrobium plants and mycorrhizal fungi, hardening seedling of the mycorrhizated dendrobium plants, and epiphytic cultivation of the dendrobium tissue culture plants and tea tree. The method improves the medical values and he economic benefit of dendrobium.

Description

technical field [0001] The invention belongs to the technical field of planting, and in particular relates to a method for imitating wild cultivation of mycorrhized dendrobium seedling tea trees. Background technique [0002] Dendrobium is a traditional precious Chinese herbal medicine in my country, which is recorded in all previous editions of Chinese Pharmacopoeia. The original plant of the traditional Chinese medicine Dendrobium is a perennial herbaceous plant of the genus Dendrobium Sw. in the family Orchidaceae. For many years, commercial Dendrobium has mainly relied on wild medicinal Dendrobium resources. With the continuous deepening of the development and utilization of medicinal Dendrobium and the increasing demand at home and abroad, the wild medicinal Dendrobium resources in my country have been severely damaged, and some areas are even facing depletion. In recent years, people have gradually begun to practice domestic cultivation and high-yield cultivation tech...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01H4/00A01G31/00
Inventor 郭景龙潘启仁吴小生刘焱晶陈春彭中胜王泽雨
Owner 贵州涵龙生物科技有限公司
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