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Application of Trichosanthes kirilowii, safflower and hemlock parsley for relieving oxidative stress injury to drosophila intestinal tract

A technology of oxidative damage and Drosophila, applied in the field of Drosophila innate immunity, can solve problems such as cancerous organisms, organ-system failure, and aging

Inactive Publication Date: 2014-07-16
NORTHEAST FORESTRY UNIVERSITY +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, when the number of stem cells changes or the function declines, it will lead to organ-system failure, induce cancer and body aging
However, there are no relevant reports on the use of the extracts of Trichosanthes quinquefolius, safflower and Chuanxiong for alleviating intestinal oxidative damage

Method used

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  • Application of Trichosanthes kirilowii, safflower and hemlock parsley for relieving oxidative stress injury to drosophila intestinal tract
  • Application of Trichosanthes kirilowii, safflower and hemlock parsley for relieving oxidative stress injury to drosophila intestinal tract
  • Application of Trichosanthes kirilowii, safflower and hemlock parsley for relieving oxidative stress injury to drosophila intestinal tract

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0018] The preparation of embodiment 1 trichosanthes quince, safflower and chuanxiong extract

[0019] Weigh 20g of trichosanthes, safflower and Chuanxiong each, add 400mL of deionized water to soak for 12h, boil at medium heat for 3h, filter, add 200mL of deionized water to the filter residue and extract once with the same method, combine the filtrate twice and concentrate to 100mL , that is, the Chinese herbal medicine aqueous extract with a mass concentration of 0.2 g / mL (20%) was obtained, and stored at -40° C. for future use.

Embodiment 2

[0020] Example 2 Effects of Trichosanthes L., Safflower and Ligusticum Chuanxiong Extracts on the Number of Intestinal Stem Cells and Enteroblasts of Fruit Flies Treated with Paraquat

[0021] Drosophila cultured in ordinary culture medium was used as the control group, and fruit flies cultured in the culture medium of Trichosanthes chinensis, safflower and Chuanxiong were used as the experimental group. Drosophila flies that had hatched for 3 to 5 days in the control group and the experimental group were randomly collected, with 15 male and female flies in each group. Each group of Drosophila was starved for 2 hours and transferred to a Drosophila tube with 5 layers of filter paper, and 6mM paraquat or 1% H was added to the filter paper 2 o 2 solution (containing 5% sucrose) to fully wet the filter paper, replace the filter paper every 24 hours and record the number of dead flies.

[0022] The results showed that after paraquat treated Drosophila for 6 days, the survival ra...

Embodiment 3

[0023] Example 3 The effects of the extracts of Trichosanthes quinquefolius, safflower and Chuanxiong on the results of intestinal cell immunostaining and the number of intestinal stem cells and enteroblasts in fruit flies treated with Paraquat

[0024] Randomly collect the female fruit flies of the control group and the experimental group that have been eclosion for 4-5 days and feed them with a paraquat (10mM) solution containing 5% sucrose for 16 hours, extract 10-12 fruit fly intestines in paraformaldehyde under a microscope, fix at room temperature for 30 minutes, and DAPI After staining for 10 min, the sections were mounted in glycerol and observed under a fluorescent microscope and photographed.

[0025] The results showed that the number of GFP-positive cells (precursor cells, including intestinal stem cells and enterocytes) in the experimental group fed with Trichosanthes, Safflower and Chuanxiong was significantly lower than that of the control group, which decreased ...

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Abstract

The invention relates to application of Trichosanthes kirilowii, safflower and hemlock parsley extracts for relieving oxidative stress injury to drosophila intestinal tract. The Trichosanthes kirilowii, safflower and hemlock parsley extracts are obtained by a water extraction method, a repairing experiment of the Trichosanthes kirilowii, safflower and hemlock parsley extracts on an oxidative injury model of the drosophila intestinal tract is performed, and the result finds that the Trichosanthes kirilowii, safflower and hemlock parsley extracts have excellent activity on the aspects of resisting oxidation, scavenging free radicals and the like, and can be used to prepare antioxidative medicaments, in particular medicaments resisting oxidative injury to the intestinal tract.

Description

technical field [0001] The invention relates to the application of the extracts of trichosanthes quince, safflower and chuanxiong in relieving intestinal oxidative damage of fruit flies, and relates to the technical field of natural immunity of fruit flies. Background technique [0002] The intestinal tract not only has the function of digestion and absorption, but also is the largest immune organ in the human body. When pathogenic microorganisms invade the intestinal tract, the intestinal innate immune system assumes a local defense role. The intestinal innate immune system mainly includes the intestinal mucosal epithelial barrier, innate immune cells, and innate immune molecules. Among them, the intestinal mucosal barrier function is an important feature of the intestinal tract. When the external environment, stress, infection and other factors stimulate the intestinal tract, the balance between the normal flora in the intestinal mucosal barrier and the host will be broke...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K36/428A61P1/00A61P39/06
Inventor 金丽华周洋
Owner NORTHEAST FORESTRY UNIVERSITY
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