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Cultivation method of halophytic grass seedlings

A cultivation method, salt-growing grass technology, applied in botany equipment and methods, cultivation, soilless cultivation, etc., can solve the problems of low seed germination rate, limited salt-growing grass, thin seedlings, etc., to prevent the loss of seeds, The effect of improving the survival rate and ensuring the survival rate

Inactive Publication Date: 2015-07-29
GANSU AGRI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Because halophytes are limited by their growth period and ecological environment, the research on the salt-tolerant mechanism of halophytes and the cloning of related salt-tolerant genes are greatly restricted. After the seedlings are thin and weak, the stress resistance is poor, and the seedling rate is low
Current research on halophytes is very limited, limited to the field of seed germination

Method used

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  • Cultivation method of halophytic grass seedlings
  • Cultivation method of halophytic grass seedlings
  • Cultivation method of halophytic grass seedlings

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0034] Embodiment 1: a kind of halophytic grass seedling cultivation method, its main feature is to comprise the following steps:

[0035] A. Seed preparation:

[0036] Sterilize the surface of Saltwort seeds with 75% alcohol for 30 seconds, remove the alcohol, let the seeds dry naturally at room temperature, mix the dried seeds with sterilized fine sand in a volume ratio of 1:10 and prepare for planting;

[0037] B. Culture substrate preparation:

[0038] Mix the sterilized fine sand and vermiculite at a volume ratio of 1:0.8 and use it as a culture substrate, put it into a culture pot, then move the culture pot into a tray, and fill the tray with distilled water to soak the culture substrate;

[0039] C. Germination of seeds covered with film:

[0040] Spread halophytic grass seeds evenly mixed with fine sand on the surface of the culture substrate, spray distilled water with a watering can to soak; then cover the culture pot with a film, and place it in a light intensity ...

Embodiment 2

[0053] Embodiment 2: a kind of halophytic grass seedling cultivation method, its main feature is to comprise the following steps:

[0054] A. Seed preparation:

[0055] Surface sterilize the seeds of saline grass with 75% alcohol for 60 seconds, remove the alcohol, dry the seeds naturally at room temperature, mix the dried seeds with sterilized fine sand in a volume ratio of 1:20 and prepare for planting;

[0056] B. Culture substrate preparation:

[0057] Mix the sterilized fine sand and vermiculite in a volume ratio of 1:1.2 and use it as a culture substrate, put it into a culture pot, then move the culture pot into a tray, and fill the tray with distilled water to soak the culture substrate;

[0058] C. Germination of seeds covered with film:

[0059] Spread halophytic grass seeds evenly mixed with fine sand on the surface of the culture substrate, spray distilled water with a watering can to soak; then cover the culture pot with a film, and place it in a light intensity ...

Embodiment 3

[0073] Salt grass seeds are surface sterilized with 75% alcohol for 60 seconds, mixed with fine sand at a ratio of 1:1 and spread on the surface of the culture substrate, sprayed with distilled water to infiltrate, covered with plastic film on the upper edge of the culture pot, and placed in a culture tray filled with distilled water . Moved into light intensity about 300ummol / m 2 , germinate in a greenhouse with a light time of 16 hours, a temperature of 22°C, and a humidity of 60%. When most of the seedlings are 1.0 cm long, the covering film is removed. The thickness of the sand covering the roots of the seedlings is about 0.4cm. Spray 1 / 2 Hoagland nutrient solution on the surface of the seedlings every day for 12 days, and a few seedlings have 4-6 leaves. The volume ratio of seedling culture substrate is fine sand: vermiculite = 1:0.8.

[0074] Under the above culture conditions, the germination rate of halophytic grass seedlings was 100%, the survival rate was 70.0%, a...

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Abstract

The invention belongs to the field of plant seedling culturing, and particularly relates to the technical field of halogeton seedling culturing, in particular to a method for culturing halogeton seedlings. The method includes the five steps of seed preparing, culturing medium preparing, seed sprouting after film covering, thin sand root covering after sprouting and thinning and nutrition supplying in the seedling stage, and manual culturing of the halogeton seedlings is achieved in the laboratory environment. According to comparison of different tests, thin sand and vermiculite are mixed at the volume ratio 1:1 to be used as halogeton seedling culturing media, the survival rate of the halogeton seedlings is higher than 70%, normal growth and normal development of halogeton are guaranteed, blossoming and seed having are achieved, single plant harvesting is achieved, and seeds for culturing next-generation seedlings are obtained. A new way is provided for the development and the use of the halogeton.

Description

technical field [0001] The invention belongs to the field of plant seedling cultivation, and specifically relates to the technical field of halophytic grass seedling cultivation. Background technique [0002] Soil salinization has become the main factor restricting agricultural production and development in my country. In addition, due to secondary salinization of soil caused by improper irrigation and other factors, the area of ​​saline soil in my country is still expanding. Most of the crops currently planted cannot survive in salinized soil, and very few crops that can survive in mildly salinized soil have poor yield levels due to the impact of saline-alkali stress. Halophytes can grow normally in salinized environment, and they are a kind of valuable genetic resources for adapting to salinized habitats. Studying the mechanism of salt tolerance of halophytes and excavating salt tolerance-related genes from halophytes have become one of the main ways to improve the salt ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01G31/00C05G1/00
Inventor 王化俊汪军成李葆春孟亚雄马小乐杨柯赖勇司二静任盼荣徐先良
Owner GANSU AGRI UNIV