Preparation method for sea cucumber polypeptide for promoting postoperation wound healing

A technology of wound healing and sea cucumber peptide, which is applied in the field of sea cucumber peptide preparation, can solve the problems of no oral administration products, achieve the effect of promoting the formation of capillaries, significantly promoting wound healing, and highlighting substantive characteristics

Active Publication Date: 2014-07-23
肽牛(北京)生物科技有限公司
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] The purpose of the present invention is to overcome the deficiencies of the above-mentioned prior art, and provide a preparation method of sea cucumber peptide that promotes postoperative wound healing; mainly to solve the problem that the existing products for promoting postoperative wound healing can only be applied externally without internal administration

Method used

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  • Preparation method for sea cucumber polypeptide for promoting postoperation wound healing
  • Preparation method for sea cucumber polypeptide for promoting postoperation wound healing

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0020] Example 1, select 100 kg of fresh Icelandic wild sea cucumber as raw material, cut open and remove internal organs such as intestines to obtain 51.2 kg of fresh ginseng body; then add 100 kg of pure water, heat to boil, and keep boiling for 8 minutes to moderately denature the protein; cool After reaching room temperature, break the ginseng body into small pieces, and reheat to 45°C to facilitate enzymolysis. After reaching the temperature, add neutral protease, the amount of enzyme added is 3000U / kg fresh ginseng body, the enzymolysis conditions are: temperature 55°C, enzymolysis time 8-12h, when the time is up, heat and boil to kill the enzyme; after killing the enzyme, cool to room temperature, and use 40-mesh silk for coarse filtration to remove large particles of impurities, add 95% (V / V) Alcohol, the alcohol content in the system reaches 60% (V / V), let it stand for 8 hours, filter with a 100-mesh filter cloth, separate the precipitate, and collect the filtrate; the...

Embodiment 2

[0072] Example 2, wild fresh sea cucumbers from Iceland were selected as raw materials, cut open and removed internal organs to obtain fresh ginseng bodies; fresh ginseng bodies were added with purified water, heated to boil, and kept boiling for 5 minutes to moderately denature the protein; after cooling to room temperature, Break the ginseng body into small pieces, and reheat to 40°C. After reaching the temperature, add neutral protease, the amount of neutral protease added is 1000U / kg fresh ginseng body, the temperature is 40°C, the enzymolysis time is 12h, and then heated to boil Kill enzymes; after killing enzymes, cool to room temperature, use 30-mesh silk for coarse filtration, add 95% (V / V) alcohol to make the alcohol content in the system reach 50% (V / V), let it stand for 5 hours, and use Filtrate with 100-mesh filter cloth, separate the precipitate, and collect the filtrate; the obtained precipitate is used for subsequent extraction of sea cucumber polysaccharides; th...

Embodiment 3

[0073] In Example 3, wild fresh sea cucumbers from Iceland were selected as raw materials, cut open and removed to obtain fresh ginseng bodies; fresh ginseng bodies were added with purified water, heated to boil, and kept boiling for 10 minutes to moderately denature the protein; after cooling to room temperature, Break the ginseng body into small pieces, and reheat to 50°C. After reaching the temperature, add neutral protease, the amount of neutral protease added is 5000U / kg fresh ginseng body, the temperature is 70°C, the enzymolysis time is 8h, and then heated and boiled Kill enzymes; after killing enzymes, cool to room temperature, use 50-mesh silk for coarse filtration, add 95% (V / V) alcohol to make the alcohol content in the system reach 70% (V / V), let it stand for 10 hours, and use Filtrate with 100-mesh filter cloth, separate the precipitate, and collect the filtrate; the obtained precipitate is used for subsequent extraction of sea cucumber polysaccharides; the obtaine...

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Abstract

A disclosed preparation method for sea cucumber polypeptide for promoting postoperation wound healing is characterized by comprising technological steps of pretreatment, heating denaturation, cooling fragmentation, heating enzyme-killing, coarse filtration and alcohol precipitation, vacuum condensation, standing precipitation, filtering and freeze drying. The prepared sea cucumber has good immunoloregulation effect on body, also has extremely substantial wound healing promotion effect, and is capable of promoting wound to heal 4-5 days early, and the healed wound is flat and no scar is left.

Description

Technical field: [0001] The present invention relates to the technical field of wound healing, in particular to a method for preparing sea cucumber peptides that promote postoperative wound healing. Background technique: [0002] In real life, some patients, such as diabetic patients, have very slow wound healing after surgery, which not only brings a lot of pain to the patient, but also greatly increases the chance of infection due to the slow healing. Therefore, how to take measures to promote surgery? It is an important topic in surgical medicine to heal the postoperative wound as soon as possible. [0003] Postoperative wound healing refers to the recovery process after the skin and other tissues at the wound site of the body are severed or damaged after surgery. It is a complex combination of various tissue regeneration, granulation tissue hyperplasia, and scar tissue formation, showing various processes. synergy. The degree of injury and the regeneration ability of t...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12P21/06
Inventor 刘凯徐莲高莹孙姿姿魏玉良
Owner 肽牛(北京)生物科技有限公司
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