Application of SAMSN1 protein in preparation of glioblastoma prognosis evaluation reagent or kit

A glioblastoma and prognosis assessment technology, applied in the biological field, can solve the problems such as no literature report, and achieve the effect of short survival period and easy death

Inactive Publication Date: 2014-07-30
SECOND MILITARY MEDICAL UNIV OF THE PEOPLES LIBERATION ARMY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] At present, there is no literature reporting that SAMSN1 is related to the pathological mechanism or prognosis of glioblastoma

Method used

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  • Application of SAMSN1 protein in preparation of glioblastoma prognosis evaluation reagent or kit
  • Application of SAMSN1 protein in preparation of glioblastoma prognosis evaluation reagent or kit
  • Application of SAMSN1 protein in preparation of glioblastoma prognosis evaluation reagent or kit

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0047] Paraffin sections of postoperative tumor tissues of 113 patients with glioblastoma and 16 cases of normal brain tissues that needed to be resected due to surgery were randomly selected (the tumor tissue sections were all from Shanghai Changzheng Hospital, and were diagnosed as glioblastoma by 2 pathologists. tumor; the normal brain tissue comes from the brain tissue of traumatic brain injury patients who need internal decompression and resection due to their illness, and the acquisition of the specimens has passed the ethical approval of the Second Military Medical University). and the expression in normal brain tissue and scored, the specific steps are:

[0048] (1) Paraffin sections of glioblastoma tissue were prepared, and the oven was set at 60° C. overnight;

[0049] (2) slices are dewaxed to water;

[0050] (Xylene ①10min→Xylene②10min→Xylene③10min→100% ethanol 5min→95% ethanol 5min→85% ethanol 5min→75% ethanol 5min→double distilled water 5min)

[0051] (3) 3%H ...

Embodiment 2

[0066] The surgically resected tumor specimens of patients with glioblastoma were randomly selected, and tissue sections of tumors of patients with glioblastoma were prepared, and the relative expression of SAMSN1 protein in glioblastoma tissues was detected by the above-mentioned immunohistochemical method test steps , to calculate the immunohistochemical score. A total of 113 glioblastoma tissues were detected, and it was found that the expression score of SAMSN1 in 77 cases (68.14%) of glioblastoma tissues was higher than 5 points. According to the expression level of SAMSN1 (when the immunohistochemical score is higher than 5, it is high expression, and if it is lower than 5, it is low expression), and the glioblastoma patients are divided into groups, and the overall survival curve is drawn ( Figure 5 A) and progression-free survival curves ( Figure 5 B). The results showed that the overall survival time and progression-free survival time of patients with high express...

Embodiment 3

[0068] Sample: a tissue section of a tumor from a patient with glioblastoma, and the relative expression of SAMSN1 protein in the glioblastoma tissue was detected by the above immunohistochemical method. Micrographs of glioblastoma tissue such as image 3 shown. The calculated immunohistochemical score of this tissue was 9.

[0069] According to postoperative follow-up, the patient's tumor recurred and died 6 months after the operation, and the survival period was only 6 months.

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Abstract

The invention relates to the field of biotechnology, and provides a new application of SAMSN1 protein, especially an application of SAMSN1 protein in preparation of a glioblastoma prognosis evaluation reagent or kit. According to the invention, an immunohistochemical method is adopted to detect the relative expression level of SAMSN1 protein in glioblastoma tissue, so as to determine the survival prognosis of glioblastoma patients. Based on the correlation of the relative expression level of SAMSN1 protein with glioblastoma, the protein can be used as a molecular marker, and detection of the expression level of the protein can guide the prognosis evaluation of glioblastoma.

Description

technical field [0001] The invention belongs to the field of biotechnology and relates to a new application of SAMSN1 protein, in particular to the application of SAMSN1 protein in the preparation of glioblastoma prognosis assessment reagents or kits. Background technique [0002] Glioma is the most common primary tumor of the central nervous system, and glioblastoma (Gliobalstoma multiforme, GBM) is the most common and most malignant type. At present, the classic pathomorphological method is still the main method for the diagnosis and classification of glioblastoma, which has many shortcomings. For example, in the case of the same diagnosis of GBM, although most patients survived for about 1 year, some patients survived for more than 3 years. Under the premise of receiving roughly the same treatment regimen, the difference in prognosis of glioblastoma patients suggests that there may be several subtypes of the tumor in terms of genetic background and pathological mechanism...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N33/68
CPCG01N33/6803G01N2800/52
Inventor 严勇陈菊祥卢亦成徐涛王洪祥秦荣
Owner SECOND MILITARY MEDICAL UNIV OF THE PEOPLES LIBERATION ARMY
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