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Application of hydroxytyrosol in anti-adriamycin cardiotoxicity and pharmaceutical composition containing hydroxytyrosol as main active ingredient

A hydroxytyrosol, anti-doxorubicin technology, applied in the directions of drug combination, active ingredients of hydroxy compounds, material excitation analysis, etc., can solve the problems of congestive heart failure, dose dependence, etc., and achieve the effect of strong protective effect

Active Publication Date: 2016-01-06
ZHEJIANG UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, doxorubicin will produce cumulative, dose-dependent myocardial toxicity, and further develop into irreversible myocardial damage, eventually leading to congestive heart failure, thus seriously inhibiting its clinical application

Method used

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  • Application of hydroxytyrosol in anti-adriamycin cardiotoxicity and pharmaceutical composition containing hydroxytyrosol as main active ingredient
  • Application of hydroxytyrosol in anti-adriamycin cardiotoxicity and pharmaceutical composition containing hydroxytyrosol as main active ingredient
  • Application of hydroxytyrosol in anti-adriamycin cardiotoxicity and pharmaceutical composition containing hydroxytyrosol as main active ingredient

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Embodiment Construction

[0054] (1) FDA and Hoechst marker cell density linear inspection

[0055] H9c2 cells in the logarithmic growth phase were digested with a digestive solution containing 0.25% trypsin and 0.02% EDTA, and were treated with different cell densities (0, 10, 100, 500, 1000, 2000, 4000, 6000, 8000, 10000 per well) were planted in the middle 60 wells of a 96-well plate, and the periphery was replaced with PBS to avoid edge effects, and placed in a cell culture incubator for 24h, 48h, and 72h respectively; the culture medium in each well was discarded, protected from light, and respectively Single-color fluorescent labeling of cells with different culture time was performed with FDA at a concentration of 10 μg / mL and Hoechst at a concentration of 10 μg / mL, incubated in a cell culture incubator for 10 min, discarded the culture medium, washed once with PBS, blotted dry, and placed under a fluorescent inverted microscope Fluorescent images are read on the platform.

[0056] The fluoresc...

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Abstract

The invention discloses an application of hydroxytyrosol in DOX (doxorubicin) cardiotoxicity resistance as well as pharmaceutical composition taking hydroxytyrosol as a main active ingredient. According to the invention, a cell membrane integrity fluorescent probe and a cell nucleus fluorescent probe are adopted to label myocardial cells simultaneously, the myocardial cell protection effect of a to-be-screened substance is surveyed from different action mechanisms, a novel application of hydroxytyrosol in DOX cardiotoxicity resistance is discovered, hydroxytyrosol has a higher protective effect on both myocardial cell membranes and cell nucleuses, and the myocardial cell protection rates of 100 mu M-hydroxytyrosol are 18.7% and 24.8% respectively under FDA (fluorescein diacetate) and hoechst33342 fluorescence labeling.

Description

technical field [0001] The invention belongs to the field of drug screening and evaluation methods, and in particular relates to the application of hydroxytyrosol in anti-adriamycin cardiotoxicity and a pharmaceutical composition with hydroxytyrosol as the main active ingredient. Background technique [0002] Hydroxytyrosol, the active ingredient in olives, features heavily in the Mediterranean diet. In early studies, it was believed that oleuropein in olive oil played an important role. After continuous in-depth research, it was found that hydroxytyrosol, the hydrolyzed product of oleuropein, has stronger physiological activity. It has a simple structure, small molecular weight, good water solubility and fat solubility, can prevent damage to the retina caused by radiation, oxygen free radicals and other factors, has good therapeutic and health functions, and has attracted extensive attention from researchers at home and abroad. [0003] Studies have shown that hydroxytyros...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61K31/05A61P9/00A61P9/10G01N21/64
Inventor 程翼宇赵筱萍王毅
Owner ZHEJIANG UNIV