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Application of neonuezhenide in resisting cardiotoxicity of doxorubicin as well as pharmaceutical composition taking neonuezhenide as main active ingredient

A new technology of privetin and anti-doxorubicin, which is applied in the direction of drug combination, anti-drugs, active ingredients of hydroxyl compounds, etc., can solve the problems of congestive heart failure, dose dependence, etc., and achieve a strong protective effect

Active Publication Date: 2014-08-06
ZHEJIANG UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, doxorubicin will produce cumulative, dose-dependent myocardial toxicity, and further develop into irreversible myocardial damage, eventually leading to congestive heart failure, thus seriously inhibiting its clinical application

Method used

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  • Application of neonuezhenide in resisting cardiotoxicity of doxorubicin as well as pharmaceutical composition taking neonuezhenide as main active ingredient
  • Application of neonuezhenide in resisting cardiotoxicity of doxorubicin as well as pharmaceutical composition taking neonuezhenide as main active ingredient
  • Application of neonuezhenide in resisting cardiotoxicity of doxorubicin as well as pharmaceutical composition taking neonuezhenide as main active ingredient

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Experimental program
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Embodiment Construction

[0054] (1) FDA and Hoechst marker cell density linear inspection

[0055] H9c2 cells in the logarithmic growth phase were digested with a digestive solution containing 0.25% trypsin and 0.02% EDTA, and were treated with different cell densities (0, 10, 100, 500, 1000, 2000, 4000, 6000, 8000, 10000 per well) were planted in the middle 60 wells of a 96-well plate, and the periphery was replaced with PBS to avoid edge effects, and placed in a cell culture incubator for 24h, 48h, and 72h respectively; the culture medium in each well was discarded, protected from light, and respectively Single-color fluorescent labeling of cells with different culture time was performed with FDA at a concentration of 10 μg / mL and Hoechst at a concentration of 10 μg / mL, incubated in a cell culture incubator for 10 min, discarded the culture medium, washed once with PBS, blotted dry, and placed under a fluorescent inverted microscope Fluorescent images are read on the platform.

[0056] The fluoresc...

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Abstract

The invention discloses an application of neonuezhenide in resisting cardiotoxicity of doxorubicin as well as pharmaceutical composition taking the neonuezhenide as a main active ingredient. A cell membrane integrity fluorescence probe and a cell nucleus fluorescence probe are simultaneously used for labelling myocardial cells, the protecting effect of a to-be-screened matter on the myocardial cell is investigated from different action mechanisms, a new application of the neonuezhenide in resisting cardiotoxicity of doxorubicin is found and is as follows: the neonuezhenide has higher protection effects on a cell membrane and a cell nucleus of the myocardial cell, and the protective percent of neonuezhenide with the concentration of 100 mu M on the myocardial cell are 29.5% and 20.4% respectively under the fluorescence labeling of FDA ( fluorescein diacetate) and hoechst33342 respectively.

Description

technical field [0001] The invention belongs to the field of drug screening and evaluation methods, and in particular relates to the application of neonaginin in anti-adriamycin cardiotoxicity and a pharmaceutical composition with neonaginin as the main active ingredient. Background technique [0002] Neoviridin, the active ingredient in olives, features heavily in the Mediterranean diet. In early studies, it was believed that oleuropein in olive oil played an important role. After continuous and in-depth research, it was found that the hydrolyzed product of oleuropein, neongorin, has stronger physiological activity. It has a simple structure, small molecular weight, good water solubility and fat solubility, can prevent damage to the retina caused by radiation, oxygen free radicals and other factors, has good therapeutic and health functions, and has attracted extensive attention from researchers at home and abroad. [0003] Studies have shown that neonrugoside can induce a...

Claims

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Application Information

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IPC IPC(8): A61K31/05A61P9/00A61P39/02
Inventor 程翼宇赵筱萍王毅
Owner ZHEJIANG UNIV