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Protein chip sample point quality control and tracing method

A protein chip and sample point technology, which can be used in material analysis by observing the influence of chemical indicators, analysis by chemical reaction of materials, optical testing of flaws/defects, etc., which can solve the problem that the quality of protein chips cannot be effectively controlled, etc. problems, to achieve significant results of technological progress

Inactive Publication Date: 2014-08-13
UNIV OF SHANGHAI FOR SCI & TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0007] In view of the defects in the above-mentioned existing protein chip preparation technology, the technical problem to be solved by the present invention is to provide a protein chip sample point quality control and tracing method, and the protein chip sample point quality control and trace method described above To solve the technical problem that the quality of protein chips in the prior art cannot be effectively controlled

Method used

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  • Protein chip sample point quality control and tracing method
  • Protein chip sample point quality control and tracing method
  • Protein chip sample point quality control and tracing method

Examples

Experimental program
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Effect test

Embodiment 1

[0025] Example 1 Quality control of a food-borne pathogenic bacteria single detection protein chip

[0026] Such as figure 1 As shown, a physical picture of a food-borne pathogen single-detection protein chip before use after spotting, including 3 parallel detection sample points, 3 parallel negative control points, and 3 parallel positive control points. Imaging processing is performed on the protein chip after the spotting is completed, and the coefficient of variation of the colored sample point of the protein chip is analyzed by image software or the roundness and uniformity of the sample point are observed and analyzed with the naked eye, so as to ensure that the coefficient of variation, roundness and uniformity are within Within an acceptable range, it will not have a large impact on subsequent quantitative or qualitative detection. figure 2 This is the actual picture of the protein chip after detection of color development.

[0027] The specific method is:

[0028]...

Embodiment 2

[0034] Example 2 Quality control of a multi-test protein chip for food-borne pathogens

[0035] Such as image 3 As shown, the physical picture of a food-borne pathogen multi-detection protein chip before use, including 3 groups of 3 parallel detection samples, 3 parallel negative control points, and 3 parallel positive controls point. Imaging processing is performed on the protein chip after the spotting is completed, and the coefficient of variation of the colored sample point of the protein chip is analyzed by image software or the roundness and uniformity of the sample point are observed and analyzed with the naked eye, so as to ensure that the coefficient of variation, roundness and uniformity are within Within an acceptable range, it will not have a large impact on subsequent quantitative or qualitative detection. Figure 4 This is the actual picture of the protein chip after detection of color development.

[0036] The specific method is:

[0037] (1) Preparation of...

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Abstract

The invention discloses a protein chip sample point quality control and tracing method which comprises the following steps: preparing a sample application buffer solution, wherein the sample application buffer solution consists of ponceaux, glycerin, Na2CO3, NaHCO3 and deionized water; manufacturing a protein chip, namely diluting a substance to be subjected to sample application to a working concentration and performing the sample application on a solid-phase substrate by adopting the sample application buffer solution, so as to obtain the protein chip; and performing image analysis, namely observing the roundness and uniformity of the sample point subjected to the sample application by adopting image software or naked eyes. A ponceaux dyeing agent is added into the sample application buffer solution, so that the buffer solution presents red, the substance to be subjected to the sample application is diluted by using the buffer solution, and the sample application is performed on the solid-phase substrate, so that a sample point on the protein chip can be observed and analyzed through the image software or naked eyes before being used. The method can serve as a quality control method during protein chip batch production.

Description

Technical field: [0001] The invention belongs to the field of biotechnology, and in particular relates to a protein chip, in particular to a protein chip sample point quality control and tracing method. Background technique: [0002] Biochip technology is a high-throughput, large-scale parallel analysis and detection technology developed in the early 1990s. It has been rated as one of the top ten scientific and technological breakthroughs of the year by Science magazine twice. It utilizes specific interactions between molecules The principle is an analysis and detection technology that integrates multiple technologies (such as biochemistry, microprocessing, microelectronics, computers, etc.). Different from traditional analysis and detection methods, the microarray formed by it makes the biochemical analysis reaction process highly integrated on a certain carrier, and high-throughput analysis and detection of hundreds of DNA, RNA, polypeptide, protein and other molecules. C...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N21/78G01N21/88
Inventor 邱实刘箐
Owner UNIV OF SHANGHAI FOR SCI & TECH
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