Precision mouse spinal cord clamping injury clamp and clamping injury force adjusting method

Abstract

The invention relates to the field of machines, in particular to a precision mouse spinal cord clamping injury clamp and clamping injury force adjusting method. The distance between the abutting side of the clamp and the clamping penetrating side of the clamp is adjusted by screwing in or screwing out an abutting screw so as to adjust the clamping force. The method comprises the following steps of selecting a female mouse with the weight ranging from 18 g to 21 g, precisely positioning the eleventh vertebral body of the mouse, conducting an osteotomy on the back vertebral plate of the vertebral body, and clamping the segment of the spinal cord to be injured in the left-right direction with a special improved vascular clamp, so that molding is successful. Compared with the prior art, the method for building the spinal cord injury model is simple and easy to implement, precise, easy to repeat, high in success rate and the like. After an injury, behavior indicators, spinal cord injury pathological indicators and glial scar indicators are observed, the method can well simulate the real process formed after the human spinal cord injury, the simulation degree is quite high, the real process is highly reproduced, and the method has the advantages beyond comparison with existing methods.

Description

technical field [0001] The invention relates to the field of machinery, in particular to a precise mouse spinal cord clamp and a method for adjusting the clamping force. Background technique [0002] Spinal cord injury (SCI) will bring irreversible neurological damage to patients. With the development of modern transportation and construction industry, the number of paraplegic patients caused by SCI is increasing. With the advancement of medical methods, the mortality rate of SCI patients has decreased, but it has brought more paraplegic patients with severely impaired quality of life. At the same time, patients and the society also need to pay huge medical expenses for life care and functional rehabilitation after SCI. Therefore, elucidating the mechanism of SCI and finding ways to reverse it is a hot spot in the field of neuroscience research. At present, the therapeutic effect on SCI is still poor, because the pathophysiological mechanism after SCI is very complex, and...

AI Technical Summary

Problems solved by technology

The body is too small, and the model of the spine and spinal cord is too detailed to construct or operate, and the error is easy to increase

③Selection of the injured segment: Because the blood vessels under the trapezius muscle are prone to massive bleeding, the mice will die of blood loss, and the vertebral body above the lumbar spine is thinner and flexes to the ventral side, which makes the operation more difficult and easily increases the error caused by the operation; If the pinched position is too low, it is difficult to obtain materials due to too many nerve fibers

④ Segment positioning: Because the thickness of each segment of the mouse spinal cord from the head to the tail is different, the same pinch thickness causes different damage degrees in different segments. In order to ensure the stability of the model, each group small The entrapment segment of the mouse should be as consistent as possible, and the traditional method of judging the segment by observing the shape of the spine is inaccurate

[0007] Moreover, the universal pinching strength cannot be adjusted, so the standards are not uniform and the evaluation is confusing

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