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Virus integration site capture sequencing analysis method

A technology for integrating sites and sequencing analysis, applied in the fields of biological information and genetic engineering technology, it can solve problems such as high price applications and achieve high sensitivity.

Active Publication Date: 2014-08-20
BGI TECH SOLUTIONS
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  • Abstract
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Problems solved by technology

Although the resolution of WGS sequencing reaches the single-base level and detects all integration events at one time, the high price at this stage still limits its application

Method used

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  • Virus integration site capture sequencing analysis method
  • Virus integration site capture sequencing analysis method
  • Virus integration site capture sequencing analysis method

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Embodiment Construction

[0030] In order to make the description of the present invention easier to understand, the technical terms used in the specific embodiments are firstly explained below.

[0031]

[0032] In order to make the object, technical solution and advantages of the present invention clearer, the present invention will be further described in detail below in conjunction with the accompanying drawings and embodiments. It should be understood that the specific embodiments described here are only used to explain the present invention, not to limit the present invention.

[0033] The present invention is applied to electronic devices with display screens and input devices (such as various medical testing devices). The display screen may be a display device such as a touch screen, and the input device may be an input device such as a keyboard. The electronic device also includes a memory and a processor connected by a data line or a signal line, and other necessary electronic components ...

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Abstract

A virus integration site capture sequencing analysis method includes the steps of: merging a human reference sequence and a virus reference sequence to construct a mixed reference sequence; reading sequencing data, and filtering the unqualified parts to obtain filtered sequencing data; aligning processed sequencing data to the mixed reference sequence by using a piece of alignment software to obtain an alignment result, and then processing the alignment result to obtain an alignment result for the detection of virus integration; according to the alignment result for detection of virus integration, executing the corresponding operations to obtain the virus integration related sequences; integrating alignment information of the above related sequences to obtain the coordinates of a virus integration site in the reference sequence; and integrating coordinate information of the integration sites and outputting the obtained results virus integration result. The method provided by the invention can obtain virus site integration information with high accuracy.

Description

technical field [0001] The invention belongs to the fields of genetic engineering technology and biological information technology, and in particular relates to a method for capturing, sequencing and analyzing virus (HBV) integration sites. Background technique [0002] Tumor viruses are mainly divided into DNA viruses and RNA viruses. The mechanism of DNA virus-induced carcinogenesis is that after the virus infects cells, the transformation protein encoded by the early gene binds or acts on the tumor suppressor protein P53 or Rb of the cell, thereby causing the inactivation of P53 or Rb, resulting in unlimited cell proliferation and uncontrolled growth. Ultimately induces cellular transformation and tumor formation. The RNA virus genome carries a viral oncogene, which causes host cell transformation and carcinogenesis through the protein produced by the transcription and translation of the viral oncogene. Certain RNA viruses that neither contain viral oncogenes nor are pr...

Claims

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Application Information

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IPC IPC(8): C12Q1/68
CPCC12Q1/6869C12Q2537/165
Inventor 丘坤龙何铭辉
Owner BGI TECH SOLUTIONS
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