Virus integration site capture sequencing analysis method

A technology for integrating sites and sequencing analysis, applied in the fields of biological information and genetic engineering technology, it can solve problems such as high price applications and achieve high sensitivity.
CN103993069AActive Publication Date: 2014-08-20BGI TECH SOLUTIONS
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Patent Information

Authority / Receiving Office
CN Β· China
Current Assignee / Owner
BGI TECH SOLUTIONS
Publication Date
2014-08-20

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Abstract

A virus integration site capture sequencing analysis method includes the steps of: merging a human reference sequence and a virus reference sequence to construct a mixed reference sequence; reading sequencing data, and filtering the unqualified parts to obtain filtered sequencing data; aligning processed sequencing data to the mixed reference sequence by using a piece of alignment software to obtain an alignment result, and then processing the alignment result to obtain an alignment result for the detection of virus integration; according to the alignment result for detection of virus integration, executing the corresponding operations to obtain the virus integration related sequences; integrating alignment information of the above related sequences to obtain the coordinates of a virus integration site in the reference sequence; and integrating coordinate information of the integration sites and outputting the obtained results virus integration result. The method provided by the invention can obtain virus site integration information with high accuracy.
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Description

technical field

[0001] The invention belongs to the fields of genetic engineering technology and biological information technology, and in particular relates to a method for capturing, sequencing and analyzing virus (HBV) integration sites. Background technique

[0002] Tumor viruses are mainly divided into DNA viruses and RNA viruses. The mechanism of DNA virus-induced carcinogenesis is that after the virus infects cells, the transformation protein encoded by the early gene binds or acts on the tumor suppressor protein P53 or Rb of the cell, thereby causing the inactivation of P53 or Rb, resulting in unlimited cell proliferation and uncontrolled growth. Ultimately induces cellular transformation and tumor formation. The RNA virus genome carries a viral oncogene, which causes host cell transformation and carcinogenesis through the protein produced by the transcription and translation of the viral oncogene. Certain RNA viruses that neither contain viral oncogenes nor are pr...

Claims

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