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HPLC (High Performance Liquid Chromatograph) quantitative detection method for raphanin of raphanin extract

A quantitative detection method, sulforaphane technology, applied in the direction of measuring devices, instruments, scientific instruments, etc., to achieve the effects of pressure reduction, sensitive detection, and good baseline

Inactive Publication Date: 2014-08-20
CHINA JILIANG UNIV
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  • Abstract
  • Description
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  • Application Information

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Problems solved by technology

[0003] Existing methods for detecting sulforaphane are also mainly high-performance liquid chromatography. HPLC is an effective method for detecting sulforaphane content. It has the characteristics of high accuracy and precision of the test results. , detection accuracy and precision still need to be improved

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  • HPLC (High Performance Liquid Chromatograph) quantitative detection method for raphanin of raphanin extract
  • HPLC (High Performance Liquid Chromatograph) quantitative detection method for raphanin of raphanin extract
  • HPLC (High Performance Liquid Chromatograph) quantitative detection method for raphanin of raphanin extract

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Embodiment 1

[0047] Embodiment 1, a HPLC quantitative detection method of sulforaphane in the crude extract of sulforaphane, the following steps are carried out in sequence:

[0048] 1), Accurately weigh the sulforaphane standard substance and dissolve it in acetonitrile-water solution (volume ratio 3:7), as a stock solution, use acetonitrile-water solution (volume ratio 3:7) to dilute the stock solution to the concentration of sulforaphane respectively A series of standard solution of 50.0, 25.0, 12.5, 6.25, 3.10, 1.50μg / mL;

[0049] 2), accurately weighed 5 mg of sulforaphane crude extract as the sample to be tested and dissolved in 10 mL of acetonitrile-water solution (volume ratio 3:7) to prepare a test solution (i.e., sample solution);

[0050] A total of 3 samples to be tested were set up, namely A, B, and C as described in Table 1.

[0051] 3), the standard solution obtained in step 1) and the sample solution obtained in step 2) were subjected to HPLC analysis respectively, and the...

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Abstract

The invention discloses an HPLC (High Performance Liquid Chromatograph) quantitative detection method for raphanin of a raphanin extract. The HPLC quantitative detection method comprises the following steps: 1, diluting a raphanin standard substance to form standard solutions with a series of concentration by an acetonitrile-water solution; 2, weighing a raphanin extract to be tested and dissolving the raphanin extract to be tested in the acetonitrile-water solution to form a sample solution; 3, respectively performing HPLC on the standard substance solution and the sample solution so as to obtain the area of absorption peaks of raphanin in the standard substance solution and the sample solution; 4, establishing a raphanin standard curve; and 5, substituting the peak area of raphanin of the sample solution in a curve equation so as to obtain the content of raphanin in the sample solution and finally obtain the content of raphanin in the raphanin extract to be tested.

Description

technical field [0001] The invention relates to a method for quantitatively detecting sulforaphane from industrialized extracts. Background technique [0002] Sulforaphane, also known as "sulforaphane", is rich in broccoli and cruciferous plants. It is a common antioxidant and the most effective and effective active ingredient among all natural anti-cancer substances. It has a unique anticancer effect. A large number of experiments have proved that sulforaphane has a good preventive effect on esophageal cancer, lung cancer, colon cancer, breast cancer, liver cancer and colorectal cancer. It can cause apoptosis and cell arrest of cancer cells, induce phase II detoxification enzymes in the human body, inhibit the production of type I enzymes, and finally discharge harmful components such as carcinogens and free radicals through a variety of enzyme systems. , this ingredient will not remain in the human body and has no side effects on the body. It is a new type of anti-cancer...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N30/02
Inventor 邓同乐葛建肖桂凤万旺军许健林芳
Owner CHINA JILIANG UNIV
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