HPLC (High Performance Liquid Chromatograph) quantitative detection method for raphanin of raphanin extract

A quantitative detection method, sulforaphane technology, applied in the direction of measuring devices, instruments, scientific instruments, etc., to achieve the effects of pressure reduction, sensitive detection, and good baseline
CN103995064AInactive Publication Date: 2014-08-20CHINA JILIANG UNIV
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Patent Information

Authority / Receiving Office
CN · China
Patent Type
Applications(China)
Current Assignee / Owner
CHINA JILIANG UNIV
Publication Date
2014-08-20
Estimated Expiration
Not applicable · inactive patent

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Abstract

The invention discloses an HPLC (High Performance Liquid Chromatograph) quantitative detection method for raphanin of a raphanin extract. The HPLC quantitative detection method comprises the following steps: 1, diluting a raphanin standard substance to form standard solutions with a series of concentration by an acetonitrile-water solution; 2, weighing a raphanin extract to be tested and dissolving the raphanin extract to be tested in the acetonitrile-water solution to form a sample solution; 3, respectively performing HPLC on the standard substance solution and the sample solution so as to obtain the area of absorption peaks of raphanin in the standard substance solution and the sample solution; 4, establishing a raphanin standard curve; and 5, substituting the peak area of raphanin of the sample solution in a curve equation so as to obtain the content of raphanin in the sample solution and finally obtain the content of raphanin in the raphanin extract to be tested.
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Description

technical field

[0001] The invention relates to a method for quantitatively detecting sulforaphane from industrialized extracts. Background technique

[0002] Sulforaphane, also known as "sulforaphane", is rich in broccoli and cruciferous plants. It is a common antioxidant and the most effective and effective active ingredient among all natural anti-cancer substances. It has a unique anticancer effect. A large number of experiments have proved that sulforaphane has a good preventive effect on esophageal cancer, lung cancer, colon cancer, breast cancer, liver cancer and colorectal cancer. It can cause apoptosis and cell arrest of cancer cells, induce phase II detoxification enzymes in the human body, inhibit the production of type I enzymes, and finally discharge harmful components such as carcinogens and free radicals through a variety of enzyme systems. , this ingredient will not remain in the human body and has no side effects on the body. It is a new type of anti-cancer...

Claims

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