Lactobacillus as well as composition and application thereof in treating autoimmune disease and complications

A technology for autoimmune diseases and complications, applied in drug combinations, allergic diseases, cardiovascular system diseases, etc., can solve problems such as the regulatory mechanism of Lactobacillus strains that have not been proposed

Active Publication Date: 2014-09-03
GENMONT BIOTECH
View PDF8 Cites 11 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0017] However, the above studies seldom explore whether Lactobacillus can be used to treat symptoms of autoimmune diseases (such as lupus erythematosus) and its complications (such as heart and liver lesions), and have not proposed the regulatory mechanism that Lactobacillus strains may be involved in.

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Lactobacillus as well as composition and application thereof in treating autoimmune disease and complications
  • Lactobacillus as well as composition and application thereof in treating autoimmune disease and complications
  • Lactobacillus as well as composition and application thereof in treating autoimmune disease and complications

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0043] Example 1: Establishment of Animal Evaluation Model

[0044] 1. Preparation of Lactobacillus strains

[0045] This embodiment utilizes Lactobacillus paracasei GMNL-32 (preservation number CCTCC M204012), Lactobacillus reuteri GMNL-89 (preservation number CCTCC M207154) or Lactobacillus reuteri GMNL-263 (preservation number CCTCC M209263) to carry out SLE Animal experiments are conducted to evaluate the effect of the above lactobacillus strains on heart and liver lesions caused by lupus erythematosus through oral administration.

[0046] The daily total bacterial count of the above three strains of Lactobacillus can be about 1×10 per gram respectively. 9 CFU / g. The above three Lactobacillus strains may be in a freeze-dried form and may contain other ingredients such as glucose, maltodextrin, infant milk powder, fructooligosaccharides, magnesium stearate, yogurt spices, other difficult-to-separate ingredients, or the above-mentioned any combination of .

[0047] 2. Es...

Embodiment 2

[0064] Example 2: Evaluation of the efficacy of Lactobacillus strains for the treatment of liver lesions caused by SLE

[0065] 1. Assess the effect of Lactobacillus strains on the liver weight of SLE animals

[0066] The SLE mouse of embodiment one is through feeding deionized water (control group), Lactobacillus paracasei GMNL-32, Lactobacillus reuteri GMNL-89 Lactobacillus reuteri GMNL-263, after 12 weeks, its liver weighs the same as The results for body weight are shown in Table 1, while the results for the weight ratio of liver weight to body weight are shown in Table 1 and figure 1 shown.

[0067] Table 1

[0068]

[0069] Refer to Table 1 and figure 1 , wherein Table 1 shows the results of the weight ratio of liver weight (g), body weight (g) and liver weight to body weight of SLE mice according to an embodiment of the present invention, and figure 1 Then draw a bar graph of the weight ratio of liver weight and body weight of SLE mice according to multiple embo...

Embodiment 3

[0077] Example three: Evaluate the effect of Lactobacillus strain on CD4 + / CD25 + Efficacy of spleen regulatory T (Treg) cells

[0078] The spleens of each group of SLE mice in Example 1 were cut into small pieces, immersed in HBSS aqueous solution (1 g / 10 mL) and DNase I (Life Technologies, Inc., USA), and reacted at 37° C. for 15 minutes. The above-mentioned HBSS aqueous solution contains collagenase type I (0.05mg / mL), collagenase type IV (0.05mg / ml), hyaluronidase (hyaluronidase, 0.025mg / ml) and soybean trypsin inhibitor (1mg / ml; Life Technologies, Inc., USA). Next, the cells were collected by centrifugation, resuspended in HBSS digestion solution, and reacted at 37° C. for 15 minutes. Then, undecomposed tissue pieces were removed by filtration, and the remaining spleen cells were washed with RPMI1640 culture medium. Afterwards, the washed spleen cells were separated using a Ficoll-Paque gradient to remove dead cells.

[0079] The resulting cells were subjected to cy...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

PUM

PropertyMeasurementUnit
pore sizeaaaaaaaaaa
Login to view more

Abstract

The invention relates to a lactobacillus as well as a composition and an application thereof in treating an autoimmune disease and complications of the autoimmune disease. The composition contains separation strains of at least one strain of the following strains in effective quantity: lactobacillus paracasei GMNL-32, lactobacillus reuteri GMNL-89 or lactobacillus reuteri GMNL-263 and a pharmaceutically acceptable carrier agent. The lactobacillus and/or the composition thereof can be used for treating the autoimmune disease and the complications of the autoimmune disease.

Description

technical field [0001] The invention relates to a lactobacillus strain and its application, in particular to a lactobacillus strain and its composition for treating autoimmune disease symptoms and complications thereof. Background technique [0002] Autoimmune disease is a disease in which the human body's own immune system attacks its own normal cells. It is a problem in which the normal immune ability is reduced, but the abnormal immune ability is highlighted. The so-called abnormal immunity is to regard friends as enemies, and attack substances in one's body that are not viruses or bacteria as viruses or bacteria, hoping to drive them out of the body. The antibody of the immune system in the human body is a physiological mechanism to protect the body. It was originally aimed at attacking and eliminating foreign antigens or abnormal cells (such as tumor cells) in the body. However, in some cases, the immune system may produce antibodies against normal cells in the body (o...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

Application Information

Patent Timeline
no application Login to view more
Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20A61K35/74A61P37/02A61P1/16A61P9/00C12R1/225
Inventor 黄志扬郭薇雯曾博修陈奕兴
Owner GENMONT BIOTECH
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Try Eureka
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap