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Culture method of transgenic soybean with OsPT6 phosphorus being efficiently utilized

A technology for transgenic soybeans and transgenic plants is applied in the field of cultivation of OsPT6 phosphorus efficient utilization of transgenic soybeans, which can solve the problems that have not yet been discovered, and achieve the effects of good growth conditions, improved expression levels, and high yields

Inactive Publication Date: 2014-09-03
NANJING AGRICULTURAL UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

But so far, there is no report on the expression of this gene in soybean, and there are few reports on the use of bioengineering to improve the efficient utilization of soybean phosphorus.

Method used

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  • Culture method of transgenic soybean with OsPT6 phosphorus being efficiently utilized
  • Culture method of transgenic soybean with OsPT6 phosphorus being efficiently utilized
  • Culture method of transgenic soybean with OsPT6 phosphorus being efficiently utilized

Examples

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Embodiment Construction

[0070] The methods used in the following examples are conventional technical means in the art unless otherwise specified, and the reagents and materials used can be obtained through commercial channels unless otherwise specified.

[0071] 1. Construction of plant expression vector pCAMBIA3301-OsPT6

[0072] The plant expression vector constructed by the present invention is based on the pCAMBIA3301 carrier, and the specific implementation methods are as follows: 1. the cloning of the rice phosphorus transporter gene OsPT6 gene:

[0073] Phosphorus-deficient rice leaves were selected as materials, and total RNA was extracted and reverse-transcribed into cDNA according to the instructions of TaKaRa’s total RNA extraction reagent (catalogue number: D9108A), and the cDNA product was digested with RNase to remove RNA residues. Referring to the sequence information of the rice phosphorus transporter gene OsPT6 (hereinafter referred to as OsPT6) (NCBI accession number: AF536966.1), d...

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Abstract

The invention belongs to the field of the molecular biology and biotechnology, relates to phosphorus translocator gene OsPT6 and an application of the gene in transgenic crops with phosphorus being efficiently utilized, provides a culture method of the transgenic soybean with phosphorus being efficiently utilized by using the gene, an expression carrier and construction containing the gene, as well as host bacteria used in the culture process as well as a measurement method and a kit for measuring relative expression quantity of the transgenic soybean OsPT6 gene. The blank that the phosphorus translocator OsPT6 gene in the soybean breeding in the prior art is solved, a plant expression carrier containing the OsPT6 gene is constructed, a CaMV35S promoter and an NOS (nitric oxide synthase) terminator are introduced into the OsPT6 gene to increase the expression regulation of the OsPT6 gene in foreign genes; the carrier further contains a herbicide resistance gene bar gene, the resistance of the transgenic plant to herbicides can be conveniently detected and is increased, the transgenic soybean produced by using the culture method provided by the invention is capable of efficiently utilizing phosphorus.

Description

technical field [0001] The invention belongs to the field of molecular biology and biotechnology, and relates to a phosphorus transporter gene OsPT6 and the application of the gene in high-efficiency phosphorus utilization transgenic crops. The expression vector and construction containing the gene, the host bacterium, and the method and kit for determining the relative expression level of the transgenic soybean OsPT6 gene used in the study. technical background [0002] Soybean is rich in protein and vegetable oil, and its high nutritional value is deeply loved by consumers. With the increasing market demand year by year, it has become an important economic crop (Wang XR, Yan XL, Liao H. Genetic improvement for phosphorus efficiency in soybean: a radical approach. Annals of Botany, 2010, 106:215-222.). At present, my country is a major soybean consumer and importer in the world, among which 58.38 million tons of soybeans were imported in 2012. Due to the impact of imported...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N15/84C12N15/66C12N1/21C12Q1/68A01H5/00C12R1/01
Inventor 朱月林盖钧镒闫闻杨立飞
Owner NANJING AGRICULTURAL UNIVERSITY